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:: Volume 14, Issue 1 (Spring 2012) ::
J Gorgan Univ Med Sci 2012, 14(1): 136-142 Back to browse issues page
Antibacterial effect of Methanolic extract of Camellia Sinensis L. on Pseudomonas aeruginosa strains producing β-lactamases
Hashemi A (MSc) * 1, Shams S (MSc) 2, Kalantar D (MSc) 3, Taherpour A (MSc) 4, Barati M (MSc) 5
1- PhD candidate in Medical Bacteriology, Shahid-beheshti University of Medical Sciences, Tehran, Iran , hashemi1388@yahoo.com
2- Microbiologist, Department of Microbiology, Qom University of Medical Sciences, Qom, Iran
3- PhD Candidate in Medical Bacteriology, Department of Microbiology, Tehran University of Medical Sciences, Tehran, Iran
4- PhD candidate in Medical Bacteriology, Shahid-beheshti University of Medical Sciences, Tehran, Iran
5- PhD Candidate in Medical Parasitology, Department of Parasitology, Tehran University of Medical Sciences, Tehran, Iran
Abstract:   (20535 Views)

Background and Objective: Pseudomonas aeruginosa is one of the important causes of nosocomial infections. Extended spectrum-beta Lactamases (ESBLs) and Metallo-beta Lactamase (MBL) producing strains have become resistant against a wide range of antibiotics. The aim of this study was to determine the effect of Methanol extract of Camellia Sinensis on Pseudomonas aeruginosa producing ESBL isolated from burnt wounds of patients.

Materials and Methods: This descriptive study was done on burnt wounds of 245 hospitalaized patients in Shafa hospital, Kerman, Iran during 2006-07. ESBLs producing strains were detected by phenotypic confirmatory test and also E-test strips were used for MBL detection. P.aeruginosa MIC was determined for Cefotaxime, Ceftazidime, Azteronam, Imipenem, Meropenem and methanol extracts of plant Camellia Sinensis prepared by Maceration method.

Results: 120 of burnt wound infected with P.aeruginosa, out of them 41 isolates contained ESBL while lacked MBL. 60% of isolates were resistant to Cefotaxime, 66% to Ceftazidime, 42% to Azteronam, 3% to Imipenem and 5% to Meropenem. Among the extracts, green Tea had the highest antibacterial effect on standard strains and P.aeruginosa producing ESBLs in 1.25mg/ml concentration.

Conclusion: This study showed that methanolic extract of green tea has higher antibacterial effect aginst β-lactamase P.aeruginosa strains than Cefotaxime and Ceftazidime.

Keywords: Pseudomonas aeruginosa, ESBL, Antibiotic Resistance, Camellia Sinensis
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Type of Study: Brief Report | Subject: Microbiology
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Hashemi A (MSc), Shams S (MSc), Kalantar D (MSc), Taherpour A (MSc), Barati M (MSc). Antibacterial effect of Methanolic extract of Camellia Sinensis L. on Pseudomonas aeruginosa strains producing β-lactamases. J Gorgan Univ Med Sci. 2012; 14 (1) :136-142
URL: http://goums.ac.ir/journal/article-1-1254-en.html


Volume 14, Issue 1 (Spring 2012) Back to browse issues page
مجله علمی دانشگاه علوم پزشکی گرگان Journal of Gorgan University of Medical Sciences
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