Azar Mohammadi, Abdolhossein Taheri Kalani, Mahnaz Omidi,
Volume 0, Issue 0 (10-2024)
Abstract
Background: When metabolic demands increase due to an obesity-induced high-fat diet (HFD), mitochondrial function is impaired, production can increase, and oxidative stress occurs. This type of stress has been shown to play a key role in various pathological conditions such as heart disease, hypertension, diabetes, chronic kidney disease, and cancers. This study aims to evaluate the impact of HFD and resistance training (RT) on oxidative stress biomarkers and cardiac health in rats.
Methods: In this experimental study, 21 male Wistar rats (weighing 200-300 g) were randomly and equally assigned into the following groups: control (CTRL), HFD, and HFD+ RT. Animals in the HFD groups received a high-fat diet for 23 weeks. During the treatments, rats in the HFD+ RT group, besides receiving a high-fat diet, performed the progressive RT protocol three times per week with 30- 100% of their body mass in the last eight weeks. At the end of the treatments, superoxide dismutase (SOD), glutathione peroxidase (GPX), total antioxidant capacity (TAC), and malondialdehyde (MDA) levels in cardiac tissue were measured by colorimetric method. The data were analyzed by one-way analysis of variance (ANOVA) and Tukey’s post hoc test at a significant level of P<0.05.
Results: HFD did not alter levels of SOD, GPX, TAC, or MDA in cardiac tissue. Cardiac SOD (P=0.021), GPX (P=0.024), and TAC (P=0.041) levels in the HFD+ RT increased significantly compared to the HFD group, but there was no significant difference in cardiac MDA levels between the three groups (P=0.438).
Conclusion: RT seems to improve cardiac tissue oxidative stress adaptations in an animal model fed with an HFD.
Rahil Shahriari, Homa Sheikhani Shahin, Mehrzad Moghaddasi, Alireza Jowhari,
Volume 11, Issue 4 (12-2023)
Abstract
Background: Non-alcoholic steatohepatitis (NASH) is one of the prevalent metabolic diseases, and knowing its treatment methods is very important. This study investigates the effect of eight weeks of combined high-intensity interval training on intrahepatic FNDC5 protein and irisin in male rats with non-alcoholic steatohepatitis.
Methods: In this study, 40 rats aged 6 to 8 weeks were divided into two groups: healthy (n=20) and high-fat diet (HFD) (n=20). After eight weeks and assurance of disease induction, the HFD group was randomly divided into control-patient (n=9) and training-patient (n=9). Also, the healthy group was divided into control-healthy (n=9) and training-healthy (n=9). The training group rats performed HIIT in aquatic and land environments (Saturdays and Wednesdays in aquatic environments and Mondays on a treadmill). Western blot method was used to measure FNDC5 and irisin proteins, and the spectrophotometric method was used to measure liver enzymes (ALT and AST). One-way ANOVA and Bonferroni's post hoc test (P<0.05) were used to determine the difference between groups.
Results: After eight weeks of combined high-intensity interval training, there was no significant difference in intrahepatic FNDC5 protein levels between the groups (P=0.125). Intrahepatic irisin protein levels significantly increased in the training-healthy group compared to the control-healthy group (P=0.046). Additionally, there was a significant increase in the training-patient group compared to the control-patient group (P=0.036) and a significant increase in the training-healthy group compared to the control-patient group (P=0.011).
Conclusion: In general, combined high-intensity interval training (aquatic + land) can increase intrahepatic irisin. Thus, this type of training can be considered one of the potential non-pharmacological options for treating NAS. However, more research is needed to reach definitive results.
