Serotonin (5-hydroxytryptamine, 5-HT) is a pivotal neurotransmitter involved in a myriad of physiological processes, including the regulation of mood, appetite, and sleep cycles. Accurate quantification of 5-HT levels in human serum is fundamental to elucidating its pathophysiology in various clinical conditions and disorders. Over the recent years, electrochemical sensing techniques have emerged as highly reliable and sensitive modalities for 5-HT detection. This article details a protocol study designed to determine 5-HT concentrations in human serum using a validated electrochemical sensing platform. The protocol encompasses a comprehensive overview of electrochemical sensing techniques, methodology validation, sample collection and processing, experimental procedures for 5-HT determination, and subsequent data analysis. The outcomes of this study hold significant potential for advancing the current understanding of 5-HT-associated disorders and may facilitate the development of novel, targeted therapeutic interventions.
