Showing 5 results for Aghaei
Zahra Moshtagh Eshgh, Ali Akbar Aghaeinezhad, Akram Peyman, Aref Amirkhani, Fakhredin Taghinejad, Ali Akbar Sheikhi,
Volume 2, Issue 2 (10-2014)
Abstract
Background & Objective: Job burnout is one of the inevitable consequences of occupational stress. Professional staff of social services are the first candidates of burnout, therefore this study was aimed to determine the relationship between occupational stresses with job burnout in pre-hospital emergency staff.
Method: In this cross-sectional study, 206 employees, working in 44 pre-hospital emergency bases in Golestan province were enrolled in years 2011-12. Information was obtained by occupational stress questionnaire and Maslach Burnout and then using descriptive statistics, Pearson correlation coefficient test, ANOVA, T-Test and Tukey tests the data were analyzed in SPSS software.
Results: 117 individuals (75.5 percent) had moderate to high stress. Organizational factors (21.62 ± 6.05) with a mean score from 100 (65.51) had the highest score in causing stress and physical factors (10.44 ± 3.43), occupational (37.12 ± 1.12) and group factors (10.54 ± 1.12) were second and third respectively. Among all the subjects, 76.6% had experienced moderate to high burnout. There was a significant statistical difference between job stress and age (P=0.02) , type of employment (P=0.048) and between burnout and education (P=0.028) in the overall level of job stress and burnout significant correlation was observed (r=0.335, P<0.001).
Conclusion: Emergency staffs work in a stressful environment, which leads to burnout, thus identifying stressors and ways to overcome these factors, especially in the corporate (management) dimension can help reduce burnout.
Faezeh Ajam, Mehrdad Aghaei, Saeed Mohammadi, Mohsen Saeedi, Nasser Behnampour, Ali Memarian,
Volume 7, Issue 2 (7-2019)
Abstract
Study objectives: Rheumatoid arthritis (RA) is a common chronic and systemic autoimmune disease, characterized by inflammation and the destruction of the joints. It is well known that CD4+ T cells play a major role in the pathogenesis of RA. Expanded subpopulations of CD4+ T cells have been reported in RA patients. Here, we investigated the expression of PD-1 on subsets of CD4+ T cells (CD4+CD28- and CD4+CD28+ T cells) in the peripheral blood (PB) and synovial fluid (SF) of patients with RA.
Methods: A total of 42 RA patients, including 10 newly diagnosed (ND) and 32 relapsed (RL) cases and also 20 healthy controls were enrolled. Phenotypic characterization subsets of CD4+ T cells were evaluated by flow cytometry, using fluorescence conjugated specific human monoclonal antibodies.
Results: The frequency of CD4+CD28+ T cells was significantly increased in SF versus PB in ND and RL patients. In contrast, the percentage of CD4+CD28- T cells was elevated in PB of ND and RL patients comparison to SF. Expression of PD-1 on CD4+CD28+ and CD4+CD28- T cells in PB of ND and RL patients was significantly higher than the healthy controls. Furthermore, PD-1 expression on CD4+CD28+ and CD4+CD28- T cells in SF versus PB of RL patients were significant increased.
Conclusion: These data suggest that CD4+ T cells subsets in RA patients were resistance to PD-1 mediated effects and PD-1 has insufficient ability to suppression of CD4+T cells.
Marie Saghaeian Jazi,
Volume 7, Issue 3 (9-2019)
Abstract
Summary:
SOX2 overlapping transcript (SOX2OT) is a long non-coding RNA associated with cancer pathogenesis. It contributes to a variety of cellular functions and recent evidence propounds its association with autophagy process. It has been showed that SOX2OT can regulate the expression of different autophagy associated factors in human cells with different mechanisms, however more remains to be investigated.
Mahmoud Mohammadi, Leila Kohan, Mohsen Saeidi, Marie Saghaeian-Jazi, Saeed Mohammadi,
Volume 10, Issue 2 (5-2022)
Abstract
Fibrosis is a common and mostly progressive pathological outcome in various chronic inflammatory disorders. Dermal (skin) fibrosis, which is associated with intense skin lesions, is a result of an uncontrolled healing process in the dermis, particularly disproportionate fibroblast proliferation and extracellular matrix (ECM) production. Animal models are substantial tools in biomedical investigations and have been considerably employed to evaluate miscellaneous features of diseases that cannot be demonstrated otherwise in humans. To date, various skin fibrosis models have been generated, including the transgene and/or genetic models and chemical and drug-induced models. However, genetic models are sophisticated and need access to convoluted methods. Accordingly, the introduction of affordable and easy to generate fibrosis models in the skin is crucial. Here, we aimed to introduce the chemical/drug-induced skin fibrosis animal models to provide an updated list of available approaches.
Zeinab Mohammadi, Marie Saghaeian Jazi , Seyyed Mehdi Jafari , Seyed Mostafa Mir , Massoud Amanlou , Jahanbakhsh Asadi ,
Volume 12, Issue 4 (4-2024)
Abstract
Background: Bone remodeling involves a balance between osteoblast-driven formation and osteoclast-mediated resorption, with disruptions leading to diseases like osteoporosis. Midazolam (MDZ), known for its sedative properties, has shown effects on cellular differentiation and hydroxyapatite formation in dental cells. However, its role in promoting osteogenic differentiation in human bone marrow mesenchymal stem cells (hBMSCs) remains unexplored, motivating this study to investigate its potential in bone regenerative therapies.
Methods: We purchased hBMSCs from Royan Institute and cultured them in complete media with α-MEM, 10% FBS, and 1% pen/strep. Cell viability was determined with MTT assay in several concentrations of MDZ (0.125 to 1 µM) for 72 hours. Osteogenic differentiation was induced over 21 days using the selected doses of MDZ with osteogenic medium. The Alizarin Red S (ARS) staining was performed to determine the calcium deposit for osteoblast cells. Data were analyzed using repeated measure ANOVA, and a p-value <0.05 was considered statistically significant.
Results: The MTT results for several concentrations of MDZ (0.125 to 1 µM) showed no cytotoxic effects on hBMSCs after 72 hours. Furthermore, ARS staining revealed increased calcium deposits in 0.5 µM MDZ compared to untreated and DMSO groups (p =0.0001). These findings suggest that MDZ promotes mineralization at lower concentrations, highlighting its potential in osteogenic applications, while higher concentrations may lack differentiating effects.
Conclusion: Midazolam promotes osteogenic differentiation of hBMSCs, particularly at 0.5 µM concentration, without cytotoxic effects. These findings demonstrate that MDZ may be a potential compound for osteoblastogenesis; however, these findings require further in vivo studies to confirm the idea.
