Volume 6, Issue 3 (9-2018)
Jorjani Biomed J 2018, 6(3): 40-47 |
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Ghovanjzadeh S, Bazzazi H, Yazdani Y. Lack of Association between PTPN22 (+1858 C>T) rs2476601 polymorphism and susceptibility to rheumatoid arthritis (RA) in Northeast of Iran. Jorjani Biomed J 2018; 6 (3) :40-47
URL: http://goums.ac.ir/jorjanijournal/article-1-622-en.html
URL: http://goums.ac.ir/jorjanijournal/article-1-622-en.html
1- Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran
2- Department of Medical Laboratory Sciences, Gorgan Branch, Islamic Azad University, Gorgan, Iran ,hadi.bazzazi@gmail.com
3- Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
2- Department of Medical Laboratory Sciences, Gorgan Branch, Islamic Azad University, Gorgan, Iran ,
3- Infectious Diseases Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Abstract: (7002 Views)
Background and objectives: Rheumatoid arthritis (RA) is an autoimmune disease with a complex genetic background. The protein tyrosine phosphatase non-receptor type 22 (PTPN22) is a lymphoid specific protein tyrosine phosphatase which is involved in negative regulation of T cell response. Several studies have assessed the association between PTPN22 single nucleotide polymorphisms (SNPs) with RA susceptibility. Here, we aimed to assess the association of PTPN22 (1858 C>T) variant with the susceptibility to RA in northeast of Iran.
Methods: A total of 127 RA patients and 119 age- and sex- matched healthy donors were enrolled. The polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) technique (PCR-RFLP) was applied to detect PTPN22 (1858 C>T) SNP. SPSS 22.0 software was used to analyze data using relevant statistical tests.
Results: Comparison of allele and genotype frequencies of PTPN22 (1858 C>T) SNP in RA patients and healthy donors revealed no significant association with RA susceptibility.
Conclusion: The present study suggests that the PTPN22 (1858 C>T) genetic variants are not associated with RA susceptibility and disease activity. While this is the first report from northeast of Iran, further studies are needed to confirm these findings
Methods: A total of 127 RA patients and 119 age- and sex- matched healthy donors were enrolled. The polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) technique (PCR-RFLP) was applied to detect PTPN22 (1858 C>T) SNP. SPSS 22.0 software was used to analyze data using relevant statistical tests.
Results: Comparison of allele and genotype frequencies of PTPN22 (1858 C>T) SNP in RA patients and healthy donors revealed no significant association with RA susceptibility.
Conclusion: The present study suggests that the PTPN22 (1858 C>T) genetic variants are not associated with RA susceptibility and disease activity. While this is the first report from northeast of Iran, further studies are needed to confirm these findings
Keywords: Protein tyrosine phosphatase non-receptor type 22 (PTPN22), Rheumatoid arthritis, rs2476601
Type of Article: Original article |
Subject:
Molecular Sciences
Received: 2018/08/15 | Accepted: 2018/10/6 | Published: 2019/01/2
Received: 2018/08/15 | Accepted: 2018/10/6 | Published: 2019/01/2
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