چکیده: (127 مشاهده)
Background: Adaptation to hypoxia can improve cardiac function and reduce cardiac complications of diabetes. This study aimed to determine the effect of HIIT on the expression of VEGF-A and HIF-1α in the hearts of diabetic rats.
Methods: Male Wistar rats (weighing 200–250 g and eight weeks old) were used in this study. Rats in the training group warmed up for 5 minutes, then performed a HIIT swimming protocol (14 repetitions of 20 seconds with 10 seconds of rest; eight weeks, three sessions per week). An external load equivalent to 7% of body weight was attached to the base of the tail in the first week and gradually increased by 1% in the following weeks (eighth week: load equivalent to 14% of body weight). Twenty-two rats were made diabetic by subcutaneous injection of streptozotocin. Seven days after injection, rats with blood sugar levels above 300 mg/dL were selected as diabetic samples. Eleven rats were placed in the healthy control group. After eight weeks, the rats were anesthetized and their hearts were removed for sampling. Gene expression was examined using real-time PCR. Data are presented as mean ± standard deviation; one-way ANOVA and Tukey's post hoc test were used.
Results: VEGF-A mRNA expression in the HIIT group increased by 60% compared to the diabetic group (p < 0.06). In the diabetic group, VEGF-A mRNA expression showed a 47% decrease compared to the control group (p < 0.001). HIF-1α mRNA expression in the HIIT group increased by about 27% compared to the diabetic group (p < 0.001). HIF-1α mRNA expression in the diabetic group decreased by about 25% compared to the control group (p < 0.001).
Conclusion: Diabetes impairs the expression of HIF-1α and VEGF-A, and HIIT increases the expression of these genes in heart tissue.
Methods: Male Wistar rats (weighing 200–250 g and eight weeks old) were used in this study. Rats in the training group warmed up for 5 minutes, then performed a HIIT swimming protocol (14 repetitions of 20 seconds with 10 seconds of rest; eight weeks, three sessions per week). An external load equivalent to 7% of body weight was attached to the base of the tail in the first week and gradually increased by 1% in the following weeks (eighth week: load equivalent to 14% of body weight). Twenty-two rats were made diabetic by subcutaneous injection of streptozotocin. Seven days after injection, rats with blood sugar levels above 300 mg/dL were selected as diabetic samples. Eleven rats were placed in the healthy control group. After eight weeks, the rats were anesthetized and their hearts were removed for sampling. Gene expression was examined using real-time PCR. Data are presented as mean ± standard deviation; one-way ANOVA and Tukey's post hoc test were used.
Results: VEGF-A mRNA expression in the HIIT group increased by 60% compared to the diabetic group (p < 0.06). In the diabetic group, VEGF-A mRNA expression showed a 47% decrease compared to the control group (p < 0.001). HIF-1α mRNA expression in the HIIT group increased by about 27% compared to the diabetic group (p < 0.001). HIF-1α mRNA expression in the diabetic group decreased by about 25% compared to the control group (p < 0.001).
Conclusion: Diabetes impairs the expression of HIF-1α and VEGF-A, and HIIT increases the expression of these genes in heart tissue.
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