Fariba Rasannezhad, Asieh Abbassi Daloii, Javad Ziaolhagh, Phd Ahmad Abdi,
Volume 0, Issue 0 (7-2024)
Abstract
Background and objectives: The purpose of the present study was to investigate the effect of aerobic exercise and psilocybin after methamphetamine induction on the gene expression of some cerebral cortex semaphorins in female Wistar rats.
Methods: In this experimental study, 40 female rats were placed in five groups; control (C), amphetamine (A), amphetamine-aerobic (AA), amphetamine-psilocybin (AP), amphetamine-psilocybin-aerobic (AAP). Methamphetamine was injected at a dose of 15 mg/kg for 5 days in the morning. Psilocybin was injected at a dose of 1mg/kg. The aerobic training program included running on a treadmill at 20-25 m/min, three days a week for eight weeks. After eight weeks, the genes expression was measured by Real Time PCR method. The data were analyzed by one-way analysis of variance and Tukey's post hoc test at a significance level of P<0.05.
Results: The results showed that the average gene expression of semaphorin 3A, semaphorin 4A and semaphorin 7A in the cerebral cortex of the A group had a significant increase compared to the C group(P=0.001). AA, AP and AAP groups showed a significant decrease in the average expression of semaphorin 3A and semaphorin 4A genes compared to the A group(P=0.001). AAP group had a significant decrease in the average expression of semaphorin 3A gene compared to AA and AP groups(P=0.001). Also, the AAP group showed a significant decrease in semaphorin 7A compared to the AP group(P=0.007).
Conclusion: According to the results, aerobic training and psilocybin supplementation can help reduce semaphorins in the cerebral cortex in rats induced with methamphetamine.
