B Rahimi-Esboei, A Pagheh, M Fakhar, S Pagheh, Y Dadimoghadam,
Volume 8, Issue 3 (8-2014)
Abstract
Abstract Background and Objective: The Outbreak of human parasitic diseases associated with the consumption of raw vegetables often occurs in both developing and developed countries. This study aimed to evaluate parasitic contamination of edible vegetables in Golestan Province. Material and Methods: This cross-sectional study was carried out in the cities of Golestan Province for six months, 2012. The samples (N = 100) were randomly chosen among different vegetables (parsley, lettuce, radish, and cress), and examined for the presence of helminthic and protozoan parasitic contaminations following washing, centrifuging and sedimentation. Results: Thirty-seven (37%) were found to have parasitic contamination, and of these 30 (81.1%) and 7 (18.9%) were helminths and protozoa, respectively. The highest rate of contamination was detected in parsley (37.9%), and the lowest in radish (12.0%). Moreover, free living larva with 58.6% and Hymenolepis nana ova with 5.1% were the highest and lowest contaminated rates, respectively. Conclusion: Based on our results, consumed vegetables in Golestan Province is considered as a potential risk for some human parasitic infections. Keywords: Vegetable, Parasite, Parasitic Infections, Golestan Province
Farid Soltani , Saman Mahdavi ,
Volume 12, Issue 6 (11-2018)
Abstract
ABSTRACT
Background and objectives: Bacillus licheniformis is a potential cause of spoilage in pasteurized products. The aim of this study was to identify and isolate B. licheniformis from commercial pasteurized fruit juices distributed in the West Azarbaijan Province, Iran.
Methods: Sixteen fruit juice samples including four apple juice and 12 orange juice samples were collected from five fruit juice manufacturing companies in Iran. The samples were tested for the presence of B. licheniformis by culture in specific media and biochemical testing. Suspected samples were also investigated for the presence of the bacterium by polymerase chain reaction using specific primer for the gyrB gene.
Results: Three samples (18.75%) from the 16 tested fruit juice samples were found as positive. In other words, one apple juice sample (25%) and two orange juice samples (16.66%) were contaminated with B. licheniformis.
Conclusion: Isolation of this bacterium indicates the unsuitable manufacturing conditions and ineffective bacterial decontamination, which might also be favorable for the growth of other fruit juice spoilage bacteria.
KEYWORDS: Bacillus licheniformis, Fruit and Vegetable Juices, Polymerase Chain Reaction.