Showing 13 results for Urinary Tract Infection
A Nazemi, M Naderi, M Jafarpour, M Mirinargesi, Sh Sharifi,
Volume 4, Issue 2 (10-2010)
Abstract
Abstract Bachground and objectives: The ability of adherence to the surface of host cell is very critical in the colonization of microbial pathogens. It has been revealed that E. coli strains that infect urinary tracts have different fimbrea such as I, S, FIC, Dr, and fimbrial adhesions. Material and Methods: In this study, 363 urine samples were obtained from patients with urinary tract infections reffered to clinical laboratories in Western areas of Tehran ,2008-2010 by using biochemical tests,200 samples were confirmed to be E.coli.First, DNA was extracted by boiling method and then the presence of fimbria fim, sfa, pap, foc, and afa genes tested by PCR. Results: In 200 samples, the frequency of fimbria fim, sfa, pap, foc, and afa genes are188 (%94 ), 34 (%17), 20 (%10), 61 (%31) and 71 (%35.5), respectively. Conclusion: The resultes show that FIM ans SFA are the most fimbrial genes of E. coli isolated from urine samples .This information can be valuable in etiology of urinary tract infection (UTI), UTI administration, and making of new vaccines. Key words: Urinary tract infection, fimbria, Uropathogenic E. coli (UPEC)
Gh Goudarzi, P Msc of Microbiology, Department of Microbiology, School of Medicine, Lorestan University of Medical, M Lashkarara,
Volume 8, Issue 3 (8-2014)
Abstract
Abstract Background and Objective: Escherichia coli, one of the most common causative agents of urinary tract infections (UTIs) acquired from community and hospital, has developed multiple resistances to various antibiotics such as aminoglycosides. The main resistance mechanism to aminoglycosides is inactivation of these drugs by a variety of acetyltransferase, nucleotidyltransferase, and phosphotransferase enzymes. this study aimed to assess the prevalence of resistance to some important aminoglycosides as well as the distribution of aph(3)-Ia, aac(3)-IIa and ant(2)-Ia genes among uropathogenic Escherichia coli isolates obtained from patients suffering UTIs. Material and Methods: Using the disk diffusion method, the antimicrobial susceptibility of 200 uropathogenic E. coli isolates collected from outpatients and inpatients was investigated to nine antibiotics. Then, the distribution of aac (3)-IIa, aph (3)-IA and ant (2)-IA genes was determined by PCR method. Results: Thirty-nine percent of E.coli isolates obtained from inpatients (n=100) and 19% of those from outpatient (n=100) demonstrated resistance to at least one of the tested aminoglycosides (i.e. 58 isolates). Among the isolates examined (n=200), 19.5%, 13%, 7.5% and 4.5% were resistant to gentamicin, kanamycin, neomycin and amikacin, respectively. The most prevalent gene among the strains resistance to at least one of the aminoglycosides (n=58) was aac (3)-IIa (65.5%), followed by aph (3)-IA (25.8%). Also, the ant (2)-IA gene was not seen in any isolates. Conclusion: The presence of aac (3)-IIa gene is significantly associated with gentamicin resistance (100%, p<0.05). Because of relatively high distribution of the aac (3)-IIa gene among uropathogenic E.coli, the use of aminoglycosides such as amikacin to treat UTI in clinical setting is recommended. Keywords: Escherichia Coli, Urinary Tract Infections, Aminoglycoside-Modifying Enzymes (AMEs)
Sedighi, I, Alikhani, My, Nakhaee, S, Karami, P,
Volume 8, Issue 4 (1-2015)
Abstract
Abstract Background and Objective: Escherichia coli is the most common cause of urinary tract infections in children and the leading cause of intra-abdominal infections (peritonitis and abscess) followed intestinal injuries. Urinary tract infection, including cystitis and pyelonephritis, is a common childhood infection. E. coli causes more than 90 percent of the community acquired and 50% of hospital acquired urinary tract infections therefore, the determination of E. coli antibiotic susceptibility is a paramount importance to clinical and epidemiological purposes. Material and Methods: In this cross-sectional study, 50 E. coli strains isolated from urine samples of children less than 7 years of age with urinary tract infections. They were compared for drug susceptibility testing by disc diffusion method with 50 strains of Escherichia coli isolated from stool samples of healthy children with the same age and sex pattern. Results: The actual amount of drug sensitivity of uropathogenic and intestinal Escherichia coli strains to amikacin was 94 and 100%, nitrofurantoin 90 and 88%, gentamicin 66 and 94%, cefixime 56 and 60%, nalidixic acid 38 and 44% and to cotrimoxazole 28 and 32%, respectively. Conclusion: the rate of resistance to gentamicin, Cefixime and nalidixic acid in urinary tract infection isolates were more than intestinal strains. The highest rate of drug resistance in urinary Escherichia coli isolates was associated with cotrimoxazole and the lowest one with amikacin. Keywords: Escherichia Coli, Intra-Abdominal Infection, Drug Resistance, Urinary Tract Infection, Children
Shahraki, Sh, Bokaeian, M, Rigi, Sh,
Volume 8, Issue 4 (1-2015)
Abstract
Abstract Background and Objective: Klebsiella pneumoniae is an opportunistic nosocomial pathogen causing a variety of infections including urinary tract infections, pneumonia, septicemia, wound infections and infections in the intensive care units. Since the ESBL producing Klebsiella pneumoniae strains are increasingly causing urinary tract infections, we aim to assess antibiotic resistance pattern and evaluate the prevalence of ESBL in Klebsiella pneumoniae isolated from urinary tract infections. Material and Methods: this cross-sectional study was conducted on 122 Klebsiella pneumoniae strains collected from Zahedan hospitals. After final identification of isolates, antibiotic susceptibility tests were carried out by using disk diffusion in agar method for 16 antibiotics and ESBL production was determined by the combined disk method. Results: The Klebsiella pneumoniae strains showed susceptibility to imipenem and amikacin ( 94.3%) ,chloramphenicol (88.5%) , gentamicin (81.1%) , ciprofloxacin (80.3%) , cefepime (73%) ,streptomycin (72.1%), nalidixic acid (68%) , tetracycline (65.6%), and cefotaxime, ceftazidime, cefpodoxime (62.3%) . The resistance of strains was seen to nitrofurantoin (53.3%), cotrimoxazole (39.3%), Cefpodoxime (37.7%), cefotaxime (36.9%), ceftriaxone (36.1%), aztreonam (34.4%), ceftazidime (32.8%). Thirty-eight isolates (31.1%) were shown to produce ESBLs. Conclusion: A high rate of resistance was observed to most of the antibiotics among ESBL producing strains therefore, it is important to be careful about the use of antibiotics and identification of ESBL using phenotypic methods. Keywords: Antibiotic Resistance, Extended Spectrum Beta-Lactamases,KlebsiellaPneumoniae, Urinary Tract Infection, Isolate
H Bagheri, F Najafi, N Behnampour, Ea Ghaemi,
Volume 8, Issue 4 (1-2015)
Abstract
Abstract
Background and objective: The periodic evaluation of antimicrobial activity of different antibiotic is essential because antibiotic sensitivity pattern may also changed during short courses. The aim of this study was to assess the frequency of Multi-drug Resistance (MDR) in Gram negative uropathogens.
Material and Methods: This study was conducted on 111 gram negative uropathogens using standard microbiology methods in Gorgan, 2011-2012. Antibiotic susceptibility was investigated by Kirby-Bauer disk diffusion methods (DDM).
Results: the most common isolates were klebsiella ( 40.5%) , Enterobacter (26.1%) , pseudomonas (13.5%) , proteus( 6.3%) , acinetobacter (1.8% ) and other gram negative bacteria ( 18.3%) .The highest antibiotic resistance was seen to clindamycin (99.1%), and the most sensitivity to Carbapenems (94.6%).Multi drug resistant was seen in 68.5% of isolates. In inpatients, all of the citrobacter species had resistant to multi drugs simultaneously.
Conclusion:a high frequency of multi drug resistant in uropathogens is observed in both inpatients and outpatients.
Keywords: Multi Drug Resistant, Gram Negative Bacteria, Urinary Tract Infection
Kargar, M, Kargar, M, Zareian Jahromi, M,
Volume 9, Issue 3 (9-2015)
Abstract
Abstract
Background and Objective: Escherichia coli O157:H7 is one of the most well-known pathogenic bacteria worldwide that can develop severe diseases such as hemolytic uremic syndrome (HUS). This study aimed to assess the prevalence of virulence genes of E. coli O157:H7 in patients with suspected urinary tract infections (UTIs).
Material and Methods: This cross-sectional study was conducted on 10,372 urine samples collected from patients with suspected UTI from six hospitals and clinical laboratories in Shiraz city. CT-SMAC medium, b-glucosidase activity test (MUG), specific antiserum, and the presence of O157 and H7 genes by PCR were used to confirm E. coli O157:H7 isolates. Then, stx1, stx2, eaeA, and hlyA genes were evaluated using multiplex PCR.
Results: In this study, 16 (7.8%) and 13 (6.3%) bacteria had O157 and H7 genes, respectively. Evaluation of virulence genes showed that genes eaeA (15.4%), stx1 and eaeA (15.4%), stx2 (7.7%), and stx2 and eaeA (7.7%) had the highest frequency in E. coli O157:H7.
Conclusion: Due to the severity of pathogenicity, low infectious dose of E. coli O157: H7, and its pathogenic genes, more extensive studies and genotyping of E. coli O157: H7 are required to be conducted in other areas of Iran in order to measure the frequency in UTIs and control the infections caused by E. coli O157: H7.
Keywords: Escherichia coli O157:H7; Urinary Tract Infections; Shiga Toxin 1; Shiga Toxin 2.
Younes Anzabi, Arash Khaki,
Volume 10, Issue 6 (11-2016)
Abstract
ABSTRACT
Background and Objectives: Many species of Ziziphora have been used in traditional medicine in the Azarbaijan region, northwest of Iran. This study aimed to determine antibacterial activity of essential oil and methanol extract of Ziziphora tenuior on some pathogenic bacteria isolated from urogenital tract infections.
Methods: The essential oil and methanol extract of Z. tenuior were obtained by Clevenger and maceration methods. Under sterile conditions, the required amount of urine was taken from patients referred to a number of clinics in Tabriz during 2014. After identification of the isolates using standard microbiological methods, antimicrobial effects of the essential oil and methanol extract of the plant on the isolates were evaluated by determining minimum inhibitory concentration (MIC), minimum bactericidal concentration, and antibiogram test.
Results: The MIC for essential oil of Z. tenuior was 250µg/mL for most Gram-negative bacteria except Pseudomonas aeruginosa. Moreover, the MIC for Staphylococcus aureus was 250µg/mL and 500µg/mL for other strains of Staphylococcus.
Conclusion: Comparison of the inhibitory and bactericidal effects of the essential oil and methanol extract of Z. tenuior showed that the essential oil is able to inhibit growth of the bacteria tested even in low concentrations. Further studies are required in this regard using animal models.
Keywords: Antibacterial Agents, Ziziphora tenuior, Plant Extracts, Urinary Tract Infection.
Mahsa Yazdi, Majid Bouzari, Ezzat Allah Ghaemi,
Volume 12, Issue 5 (9-2018)
Abstract
ABSTRACT
Background and objectives: Urinary tract infections (UTIs) are one of the most common infectious diseases caused by bacteria. The primary etiologic agent of UTIs is Escherichia coli. Uropathogenic E.coli (UPEC) strains have a number of specific virulence factors, which can worsen UTIs. This study was performed to detect fim, pap, sfa and afa genes among E.coli strains isolated from UTIs.
Methods: A total of 100 E. coli isolates from patients with UTI was collected between June and December 2015 from Mosavi and Sayyad Shirazi hospitals in Gorgan, Iran. All bacterial isolates were identified via standard biochemical testing and Gram straining. Presence of the genes was assessed by polymerase chain reaction.
Results: The frequency of the fim, pap, sfa and afa genes was 100%, 79%, 69% and 8%, respectively. All isolates contained at least one virulence gene. Prevalence of multiple adhesion genes was 6% for all genes and 65% for three genes (fim, pap and sfa) together. In addition, the frequency of the fim gene was significantly higher than that of the other genes (P<0.0001).
Conclusion: The results of this study indicate the high prevalence of virulence factors that can enhance pathogenicity of E. coli. Therefore, these factors could be used as diagnostic markers or vaccine targets.
Keywords: Virulence factors, Urinary tract infection, Uropathogenic Escherichia coli.
Maryam Rafiee, Alijan Tabarraei, Mahsa Yazdi, Alireza Mohebbi, Ezzat Allah Ghaemi,
Volume 17, Issue 2 (3-2023)
Abstract
Background and objectives: Urinary tract infection (UTI) is one of the most common bacterial infections. Staphylococcus saprophyticus is a common Gram-positive bacterium that causes uncomplicated UTIs in women. The present study aimed to study the drug resistance pattern and phenotypic and genotypic variation of S. saprophyticus isolates from women with UTI in Gorgan, northern Iran.
Methods: This study was performed from May 2018 to September 2020. During this time, 35 S. saprophyticus strains were isolated from patients with UTI. The antimicrobial patterns of the isolates were determined by a conventional method. Phenotypic criteria such as pigment production, mannitol fermentation, urease production, and 16SrRNA gene valuation were studied.
Results: All isolates were sensitive to nitrofurantoin, gentamicin, and linezolid. S. saprophyticus isolates showed the highest level of resistance to penicillin (85.7%) and erythromycin (51.4%). A variation was detected among S. saprophyticus isolates in terms of pigment production i.e. about 51.4% showed yellow pigment in Muller Hinton agar, and 62.9% of the isolates were able to ferment mannitol sugar. Of 11 isolates that were sequenced for the 16SrRNA gene, only two isolates showed different patterns.
Conclusion: Nitrofurantoin and trimethoprim-sulfamethoxazole are the antibiotics of choice for the treatment of UTI caused by S. saprophyticus in the study area. Due to the phenotypic and genotypic differences among S. saprophyticus isolates, typing of S. saprophyticus at the subspecies level is recommended.
Fatemeh Rashedi, Zahra Yazdanpour, Farzad Khademi, Hamid Vaez,
Volume 17, Issue 6 (11-2023)
Abstract
Background: Urinary tract infection (UTI) is one of the most prevalent bacterial diseases worldwide. Escherichia coli is a well-known etiological agent of UTI. The emergence and spread of metallo-beta-lactamase (MBL)-producing E. coli is a serious threat to public health.
This study aimed to investigate the antibiotic resistance pattern and prevalence of MBL-producing E. coli isolated from UTI.
Methods: From January 2020 to June 2021, 1200 urine specimens were collected from patients suspected of having UTI. Antibiotic susceptibility testing was carried out by the disk diffusion method. The prevalence of MBL (blaVIM, blaIMP, blaSPM, and blaNDM) genes was determined by the polymerase chain reaction (PCR) method.
Results: The highest susceptibility was observed against amikacin (96%) and gentamicin (95%). The isolates were mostly resistant against ampicillin (72%) and cephalothin (60%). All carbapenem-resistant isolates were MBL-positive. Based on the results of PCR, 75% of the isolates were blaNDM-positive.
Conclusion: Resistance to some antibiotics, such as ampicillin and cephalothin, was high, and their prescription must be restricted. The prevalence of MBL-producing isolates was not high; however, due to the high level of resistance against other antibiotics, continuous monitoring of MBL-producing isolates is highly essential.
Abolfazl Shirdel Abdolmaleki, Abolfazl Rafati Zomorodi, Mohammad Motamedifar, Yalda Malekzadegan,
Volume 18, Issue 1 (1-2024)
Abstract
Background: Urinary tract infection (UTI) is one of the most common bacterial infections of all ages and sexes. Escherichia coli is reported as the most common predominant pathogen. Urinary tract infection treatment leads to abundant antibiotic application in hospitals and communities, continuously developing multidrug resistance (MDR). This study aimed to determine the sensitivity and resistance pattern to common antibiotics among E. coli isolates from patients with UTIs at Nemazee Hospital in Shiraz.
Methods: This retrospective cross-sectional survey studied 1910 positive urine samples with E. coli bacteria from patients referred to Nemazee Hospital from 2018 to 2019. Antimicrobial susceptibility testing was performed on 12 commonly used antibiotics for UTIs.
Results: A total of 1910 E. coli isolates were gathered during these 2 years. The most highlighted resistance was observed against quinolones and cephalosporins at 86.9% and 89.7%, respectively. Cephalexin (87.9%) and nalidixic acid (86.1%) have shown the lowest activity against E. coli isolates. Also, the highest susceptibility was determined for amikacin (88.3%), nitrofurantoin (76.8%), and gentamicin (70.6%). In addition, 1624 (85%) isolates were MDR.
Conclusion: In conclusion, resistance to antibiotics (such as ciprofloxacin, norfloxacin, tetracycline, cefotaxime, and nitrofurantoin) is increasing. Therefore, it is vital to follow an appropriate antimicrobial stewardship program.
Hina Rahangdale, Tejaswini Olambe, Priyanka Klabhor, Sangita Bhalavi, Varsha Wanjare, Sunanda Shrikhande,
Volume 18, Issue 2 (3-2024)
Abstract
Background: Urinary tract infections (UTIs) are among the most common types of infections affecting people in community and hospital settings. Bacteria are the leading cause of UTIs, followed by fungi. 39% of all healthcare-associated infections (HAIs) affecting all age groups are UTIs, causing high morbidity and mortality rates. The antibiotic susceptibility pattern of causative organisms is changing due to improper antibiotic use. The study was conducted to determine the microbiological profile of both community and HAIs and their antimicrobial susceptibility pattern.
Methods: Clean-catch, mid-stream urine samples collected in the universal wide-mouthed sterile containers were transported to the laboratory. Samples were processed by standard conventional microbiological procedures. Antimicrobial susceptibility was done using the Kirby-Bauer disc diffusion method on Mueller-Hinton agar plates.
Results: The most common causative organisms among gram-negative bacteria were E coli (26.05%), followed by Klebsiella spp (20.37%), and Enterococcus spp (12.81%) was more common among the gram-positive bacteria. Non-albicans Candida (64.10%) were more commonly isolated than Candida albicans (35.90%). E coli was highly susceptible to nitrofurantoin and fosfomycin, and Klebsiella spp and Enterococcus spp were similarly highly susceptible. Antibiotic resistance was more common among bacteria isolated in HAIs.
Conclusion: In both settings, E coli was the most common causative organism. The incidence of non-albicans Candida species has increased in comparison to Candida albicans. Antimicrobial susceptibility to empirical 3rd-generation cephalosporins and fluoroquinolones has drastically decreased. Hospital-acquired UTIs are a rising threat to the healthcare system and community. Based on hospitals’ antimicrobial policy formulated by studying antimicrobial susceptibility patterns, empirical treatment should be chosen.
Deepa Devhare, Sae Pol,
Volume 18, Issue 3 (5-2024)
Abstract
Background: Vancomycin-resistant enterococci (VRE) has become a growing concern in healthcare settings as a major cause of many nosocomial infections worldwide. Risk factors associated with VRE are important to study. High-risk patients need to be screened and isolated to prevent the spread of infection and colonization. The present study aims to investigate the clinical spectrum, risk factors, and source of transmission of VRE in infected and colonized patients.
Methods: A prospective observational study was carried out for 1 year. A total of 200 Enterococcus species isolated from clinical samples such as urine, pus, blood, sterile body fluids, and stool from 200 patients without infection were included in the study. Stool samples were screened to measure the prevalence of VRE colonization. All samples were screened for vancomycin resistance using the Kirby-Bauer disc diffusion method. Vancomycin MIC was detected using the macrobroth dilution method. Demographic and clinical history of the patients were recorded.
Results: Vancomycin resistance was detected in 7 (3.5%) of 200 enterococci isolates from clinical samples. Urinary tract infection (n = 5, 71.4%) was the most common clinical illness caused by VRE. Gut colonization was found in 12 (6%) out of 200 patients screened for VRE. A history of previous antibiotic exposure was a significant risk factor in the current study and was associated with VRE infection and colonization. Endogenous bloodstream infection caused by VRE was found in one patient with VRE colonization.
Conclusion: The findings of this study highlight the significant burden of VRE on patients, both those infected and colonized. The emergence of multidrug-resistant bacteria in healthcare settings, a consequence of inappropriate antibiotic use, is a serious concern that warrants further research and our continued attention.
