Mohajerani, Mr, Sarikhani, A, Gandomani, M, Eslamirad, Z, Mosayebi, M, Didehdar, M,
Volume 8, Issue 5 (1-2015)
Abstract
Abstract Background and Objective: Malassezia that is a part of normal flora is lipophilic yeast involved in a variety of skin diseases such as seborrheic dermatitis, pityriasis versicolor, atopic dermatitis and psoriasis. Seborrheic dermatitis affects most often the sebaceous-gland-rich areas of skin such as face, scalp, and parts of the upper trunk. Dandruff is a mild variant of seborrheic dermatitis characterized by scaling. In this study, Malassezia species causing dandruff were identified. Material and Methods: In this descriptive study, the samples (n= 60) from participants with dandruff were examined under a microscope using 10% KOH solution and cultured in Leeming and Notman ager medium. DNA Extraction was performed from colonies by glass bead and the Malassezia genus, and species were detected by CfoI enzyme using PCR-RFLP method Results: Of 60, 40 (66.6%) were positive for Malassezia yeast. The positive samples in direct examination grew in culture medium. Malassezia species isolated were Malassezia globosa (25 cases), Malassezia restricta (10 cases), Malassezia furfur (3 cases) and Malassezia sympodialis (2 cases). Conclusions: In most studies, the Malassezia species were identified as the agents causing seborrheic dermatitis. In our study, Malassezia globosa was isolated as a dominant species. Keywords: Seborrheic Dermatitis, Malassezia SPP, Arak
A Raefi, N Nasrollahi Omran, A Nazemi,
Volume 9, Issue 2 (7-2015)
Abstract
Background and Objective: Malassezia yeast is considered lipophilic normal flora of human skin and warm-blooded vertebrates. This fungus is an opportunistic pathogen in causing seborrheic dermatitis. In this study, the yeasts isolated from the crust of the patients with seborrheic dermatitis were identified by PCR-Sequencing.
Material and Methods: In this study, 65 samples of the skin of ear, nose and dandruff were cultured in selective medium Sabouraud agar and modified Dixon agar to prevent dehydration. After biochemical tests, ITS1-4 Universal PCR primers were used to determine the species of yeast. Obtained PCR products were sequenced for the determination and identification of Malassezia species.
Results: Of nine samples obtained from scalp, four were Malassezia globosa, two Malassezia restricta, two Cryptococcus albidus and one Cryptococcus albidus milis.
Conclusion: The results of Malassezia globosa and Malassezia Restericta are very similar with those in studies elsewhere.
Keywords: Malassezia, Sequencing, Seborrheic Dermatitis, Tonekabon
