A Khaki,
Volume 8, Issue 2 (7-2014)
Abstract
Abstract
Background and Objectives: Ciprofloxacin is a synthetic antibacterial agent belonging to Fluoroquinolone drugs affecting effectively on gram-negative bacterial infectious. The aim of this study was to assess the effect of ciprofloxacin in the spermatogenesis period.
Material and Methods: The subjects were 20 male wistar rat randomly divided in to control (n=10) and experimental group (n=10), given 12.5mg/kg ciprofloxacin (soluble in drinking water) in spermatogenesis period. On the day of 28, the sperm was collected from cauda epididymis and sent for analysis.
Results: Based on light microscopic observation and statistical analysis, the majority of seminiferous tubules of control group were healthy, in Sc 8-9 stage. But in test group, sertoli cell degeneration and absence of sex cells were confirmed, and in some parts, just basal layer of seminiferous tubule was remained in Sc 3-5 stage.
Conclusion: Sex hormones (LH and FSH) and spermatogenesis (sperm count, motility and viability) were significantly decreased in test group compared to those of controls (P<0.05). Ciprofloxacin has some adverse effects on sperm related variables in 28 day period.
Keywords: Ciprofloxacin, Sperm, Sex Hormones
Nakisa Zarrabi Ahrabi, Hedyeh Ghadiripour, Seyed Mehdi Tabaie,
Volume 16, Issue 5 (9-2022)
Abstract
Background and objectives: Human Dermal Fibroblasts (HDF) are involved in the production of the extracellular matrix, formation of the connective tissue, and wound healing. Considering the role of ciprofloxacin in the treatment of skin infections and the subsequent oxidative stress as well as the protective effects of vitamin E and low power laser against inflammation and oxidative stress, we evaluated combined effects of low power laser and vitamin E on inflammation and oxidative stress in HDF cells treated with ciprofloxacin.
Methods: Morphology of the cells was studied using an inverted microscope. Viability of the cells was assessed using the MTT assay, and the concentration of reactive oxygen species was determined after exposure of the cells to ciprofloxacin (5, 25, 50, 75, and 100 μg/ml), vitamin E (1 mg/ml), and low power laser (660 nm; power density: 30 mW.cm−2).
Results: The survival rate of the cells increased significantly after the treatment with ciprofloxacin, vitamin E, and low power laser compared with the cells treated with ciprofloxacin and vitamin E (p<0.001). The amount of reactive oxygen species increased in the treated cells when compared with those only treated with ciprofloxacin and vitamin E.
Conclusion: The low power laser treatment has favorable effects on the growth of HDF cells, which can be beneficial for wound healing, even in the presence of ciprofloxacin.
