Showing 5 results for Antioxidants
H Tavilani, R Setarehbadi, A Fattahi, Sh Nasrollahi, J Karimi, G Shafiee, M Hosseinipanah,
Volume 7, Issue 4 (1-2014)
Abstract
Abstract
Background and objective: There is increasing evidence for the role of oxidative stress in female reproductive tract. The purpose of this study was to determine the activity of antioxidant enzymes during menstrual cycle. In addition, the relationship between activity of antioxidant enzyme and sex hormones was evaluated.
Materials and methods: In this study the activity of superoxide dismutase, glutathione peroxidase, glutathione reductase, catalase and total antioxidant capacity during the menses, follicular and luteal phases of the menstrual cycle in twenty women with regular menstrual cycle were studied. Furthermore, the correlation between activity of antioxidant enzymes and estradiol, progesterone, LH, FSH and testosterone were evaluated.
Results: There was no significant difference between activity of superoxide dismutase, glutathione peroxidase, glutathione reductase, catalase and total antioxidant capacity during the menses, follicular and luteal phases of the menstrual cycle (P>0.05). We found significant correlation, in luteal phase, between superoxide dismutase and FSH (P<0.05، r=0.44) and LH P<0.05،r=0.54). Also it is observed between LH and glutathione peroxidase (P<0.05، r=0.44).
Conclusion: Based on the results, there is no significant difference between antioxidant enzymes and total antioxidant capacity of plasma during menstrual cycle. In other words, physiologic system of women with regular menstrual cycle can protect body against oxidative stress and this is probably performed due to action of FSH and LH hormones.
Keywords: Antioxidants Menstrual cycle Sex hormones
Amir Mohammadi , Masoume Mazandarani , Jila Asghari (phd),
Volume 10, Issue 3 (5-2016)
Abstract
ABSTRACT
BACKGROUND AND OBJECTIVE: Stachys inflata Benth. is used as an anti-inflammatory and antiseptic agent in traditional medicine in most mountain villages of Golestan province. Therefore, this study aimed to investigate the antioxidant, ethnopharmacological and phytochemical properties of extract from different parts of S. inflata, collected from Chahar Bagh Mountain.
METHODS: Flowering branches and root of the plant were collected from Chahar Bagh Mountain (2100 m) in July 2013. At the same time, the most important information about traditional uses of the plant (ethnopharmacology) was recorded by questioning local people. Phytochemical evaluation (total phenolic, flavonoid and anthocyanins content) of ethanolic extract of plant organs was done using spectrophotometry and folin-ciocalteu. The antioxidant activity of the extract was evaluated by DPPH test. P ≤0.05 was considered as statistically significant.
RESULTS: The amount of chemical compounds in the extract of flowering branches and root extract was significantly different. The total phenolic (129.96 ± 5.6 mgGAE/g), flavonoid (29.62 ± 1.4 mgQUE/g) and anthocyanin (0.021 ± 0.001 µg/g) content in the extract of aerial parts of the plant was approximately 1.5 to 3 times higher than those in the root. Due to higher production of active compounds, the antioxidant activity of the aerial parts’ extract showed a greater potential in free radical scavenging (IC50= 76.33 ± 4.2 µg/ml) compared to the root extract.
CONCLUSION: Phytochemical findings and antioxidant activity of the extract of aerial parts of the plant in free radical scavenging, confirm the traditional applications of this plant as analgesic, anti-inflammatory and antiseptic agent in treatment of rheumatism, wounds, burns and diarrhea. It is recommended that further evaluation of the plant’s traditional applications be conducted in vivo and in vitro.
Seyed Zanyar Athari, Mir Alireza Nourazar, Mohammadreza Nasirzadeh,
Volume 16, Issue 3 (5-2022)
Abstract
Background and objectives: Increased oxidative stress and altered antioxidant levels play an essential role in the pathogenesis of diabetes mellitus. Menopause is associated with increased food intake, weight gain, metabolic disorders, and increased level of inflammatory and oxidative stress factors. This study evaluated effects of oleuropein extract on brain tissue and serum oxidative status in ovariectomized diabetic rats.
Methods: In this study, 40 female Wistar rats weighing 250±20 grams were randomly divided into four groups: control, sham (surgery without ovariectomy), ovariectomy+diabetes, and ovariectomy+diabetes+oleuropein supplementation. Diabetes was induced by intraperitoneal injection of streptozotocin. The rats in the treatment group received 60 mg/kg of oleuropein for 30 days by oral gavage. Finally, blood glucose, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and malonaldehyde levels were evaluated in serum and brain tissue samples.
Results: Weight, blood glucose level, and antioxidant enzymes activity increased significantly in the ovariectomy+diabetes group compared to the control group.
Conclusion: This study suggested that the administration of oleuropein extract has beneficial effects on blood glucose level and antioxidant status in serum and brain tissue of ovariectomized diabetic rats.
Mark Aihiokhai, Augustine Okpiabhele,
Volume 16, Issue 5 (9-2022)
Abstract
Background and objectives: Piper guineense (African black pepper) is a widely consumed spice in some parts of Central and Western Africa, especially Nigeria, due to its nutritional and medicinal properties. This study investigated biochemical effects of extract of P. guineense seeds on lipid profile, renal function parameters, and antioxidant status of Cavia porcellus.
Methods: Forty guinea pigs (weighing 200–300g) were equally divided into a control group and three experimental groups. Animals in the control group received 1 mL of distilled water daily for 28 days, while animals in the experimental groups 1, 2, and 3 received 0.5 mL of distilled water along with 10, 20, and 20 mg/100 g body weight powered seeds daily for 28 days.
Results: Phytochemical studies revealed the presence of flavonoids, alkaloids, tannins, saponins, and calcium. The median lethal oral dose of the aqueous extract of P. guineense seeds was greater than 5,000 mg/kg, indicating a high degree of safety. The extract at dose of 30 mg/100 g body weight had the most beneficial effects on lipid profile, renal function parameters, and antioxidant status of guinea pigs when compared with other groups.
Conclusion: Given the beneficial effects of P. guineense on lipid profile and renal function parameters, the plant can be incorporated in daily diets to ensure receiving adequate amount of essential vitamins, phytochemicals, and other nutrients present in the plant.
Usunobun Usunomena, Ambrose Emuobonuvie Akpovona ,
Volume 18, Issue 5 (9-2024)
Abstract
Background: Telfairia occidentalis is among the most popular vegetable crops propagated in the West African rainforest zone for its green leafy vegetable and ellipsoidal fruit, which are highly nutritious. This study investigated the liver's synthetic ability and hematological profile potential of T. occidentalis against carbon tetrachloride-induced toxicity in Wistar rats.
Methods: Five experimental groups of rats were used in this study. One group received distilled water and served as the normal control. The second group received carbon tetrachloride (CCl4) alone for four days. The third and fourth groups received CCl4 for four days prior to treatment with 200 mg/kg and 400 mg/kg T. occidentalis aqueous extract for six days, respectively. The last group received CCl4 for four days prior to treatment with silymarin (100 mg/kg) for six days. With the exception of normal control rats, all rats received a mixture of freshly prepared CCl4 in olive oil (1 ml/kg, 1:1 intraperitoneally) for four days. The activities of liver synthetic molecules, such as total protein, albumin, and total bilirubin, as well as hematological parameters, were measured in the blood.
Results: CCl4 exposure and toxicity caused a significant (P < 0.05) increase in total bilirubin and white blood cells and a significant decrease in total protein, albumin, hemoglobin, hematocrit, red blood cells, and platelets. However, treatment with T. occidentalis aqueous extract significantly (P < 0.05) ameliorated the levels of these markers toward normal values.
Conclusion: T. occidentalis aqueous extract exhibited enhancement of liver synthetic ability and hematological profile in CCl4-induced toxicity.
