Showing 12 results for Antibody
H Bazzazi, M.a. Ramezani, M Bazoori, A Mohamadi Bondarkheili, M Arabahmadi, A Ghaemi,
Volume 1, Issue 1 (4-2007)
Abstract
Abstract
Background and objectives:
most common causes of morbidity and mortality in industrial and
developing countries. Recent studies have suggested that
A coronary heart disease is one of the
Helicobacter pylori
heart disease therefore, this study was carried out in Gorgan, Iran, to
show the relationship between coronary disease and Helicobacter
pylori infection.
caused infection may be associated with chronic
Materials and Methods:
carried out on 109 patients suffering from acute coronary syndrome
and 85 healthy individuals, ELISA was used to determine Anti
Helicobacter pylori Anti bodies (IgA, IgG ).
In this cross sectional case-control study
Results:
while in control group were %32.9 and %62.4. There was significant
difference between IgA of two groups (p<0.007). Simultaneous
presence of both IgG and IgA in patients affected by Coronary disease
was meaningful (p<0.003).
IgA and IgG antibodies of case group were %51.4 and %53.2
Conclusion
be related to coronary disease, we suggest their investigation in
suspected individuals.
: Since Simultaneous presence of both IgG and IgA may
Key word
Antibody
: Acute Coronary syndrome, Helicobacter Pylori,
A Moradi,, A Ahmadi, S Bakhshandeh-Nosrat, E Sanee- Moghaddam, M Saeedi,
Volume 1, Issue 1 (4-2007)
Abstract
Abstract Background and objectives: HTLV-1 virus belongs to the retrovirus and infection with this virus mostly is seen among people having more than one time blood transfusion. Because of requiring repeated blood transfusions, thalassemic patients are considered to be high risk subjects in this regard. Thus, this study was carried out to indicate the frequency of HTLV-1 infection among the thalassemic patients. Materials and Methods: Blood samples of 181 thalassemic patients referred to Taleghani hospital during nearly two years (2004-2005) were taken. By using ELISA technique, the sera were assessed to determine HTLV antibody. The positive ones subsequently were examined by western Blot (kit, 2.4) to confirm the ELISA positive samples and also to recognize the HTLV type. Results: Of 181 thalassemic patients, 93 (51.4%) were male. The age was between one and twenty five (14.11 ± 6.5). 93.4% (169) were received packed cell only once in a month. 14.9% (27) were HTLV positive by ELISA technique, while just eight out of these 27 were considered to be true positive by Western blot and to be contaminated by type one virus. Of all subjects, 4.4% were positive HTLV1. Furthermore, the contamination with this virus is increased as the patients getting older. Conclusion: The findings indicated that among the thalassemic patients in Gorgan, there are cases with HTLV-1 whose frequency is correlated with the other part of our country. Consequently, further comprehensive studies are required to identify those infected blood donated to minimize the transmission risk of this infection in the society and in particular among the people receiving blood, such as thalassemic patients. Keywords: HTLV-1 antibody, thalassemic patients, ELISA, western Blood, Gorgan Journal
M Mofidi, M Saeedi, N Behnampoor,
Volume 1, Issue 2 (10-2007)
Abstract
Abstract Background and objectives: Herpes simplex virus type two (HSV-2) infections are mostly considered as a sexually transmitted disease. It causes genital herpes, newborn herpes non-pyrogenic meningitis and may act as an underlying factor for HIV infection. This study was conducted to determine the sero-epidemiologic prevalence of herpes simplex virus type two in cases referred to ten clinical laboratories of Gorgan. Materials and methods: This cross- sectional study was performed on blood Samples of 406 randomly selected Clients. These samples were analyzed for Anti HSV-2 using Specific enzyme-Linked immunoassays (ELIZA) Results: Four point nine percent of all subjects have anti HSV-2. There is Significant difference between sero-positive HSV-2 and factors such as age and Marital Status (P<0.05). Conclusion: HIV is progressively increased and HSV-2 infection is one of the major risk factors for HIV therefore, decreasing the prevalence of HSV-2 infections may Leads to the reduction of HIV prevalence. Key word: HSV-2, Antibody, Gorgan, Sero-epidemiology.
M Hedayat Mofidi, A Moradi, M Saeedi, N Behnampoor, J Arab Yaramohammadi,
Volume 2, Issue 1 (4-2008)
Abstract
Abstract Backgrounds and objectives: Herpes simplex virus type 1 (HSV- 1) infections are mostly shown as a Herpes disease, but It causes conjunctivitis, genital herpes, encephalitis and newborn herpes. This study was conducted to determine the sero-epidemiologic prevalence of herpes simplex virus type 1 in cases referred to clinical laboratories of Gorgan, Iran. Material and methods: In this cross sectional study, we did random blood sampling on 406 cases referred to the Gorgan city's clinical laboratories. These samples were analyzed for HSV-1 Immunoglobulin G and M antibodies using type- specific enzymelinked Immunoassays (ELISA). Results: Of 406 participants, the HSV-1 seroprevalence is 49% (44.3% and 4.7% for IgG and IgM respectively). There is not significant relationship between seropositive HSV-1 and gender, ethnicity, age and marital status. Conclusion: Sero-epidemiological of HSV-1 in Gorgan is the same of the other places in Iran, but it is higher than European and lower than African countries. It seems that the people’s culture is very important. Therefore it needs to be investigated more. Keywords: HSV-1, Antibody, Gorgan.
Bazzazi, H., Govahi, M., Jahazi, A., Alizadeh, Sh., Naeimi Tabiee, E, Mokaram, R., Davarpanah, M R,
Volume 6, Issue 2 (10-2012)
Abstract
Abstract
Background and objectives: Recurrent miscarriage (RM) is one of the most common reproductive disorders, generally considered to be the loss of three or more pregnancies before viability. One of the causes of this disorder is the immunological factors such as autoantibodies associated with anti-phospholipid syndrome.
Material and Methods: this case-control study was conducted on 80 pregnant women divided into two equal groups of healthy and RM women in Gorgan. The subjects were asked to fill out a questionnaire and examined by a gynecologist to distinguish their condition, healthy or RM. The blood samples were kept at -20c and assessed by both ELISA 96 and automated ELISA processor with single dose ready-to-use test to measure β2GP1 autoantibodies of IgM and IgG.
Results: the results indicate that % 7.5 of RM patients are seropositive for Anti-β2GP1 IgG, and % 5 for Anti-β2GP1 IgM , but in healthy pregnants , % 7.5 are seropositive for Anti-β2GP IgG , and %7.5 for Anti-β2GP1 IgM. Family history of abortion in RM group (33.5%) is significantly higher than healthy one (17.5%).
Conclusion: based on the results, the difference between the level of Anti-β2GP1 IgM and Anti-β2GP IgG in case and control groups is not significant, but the production of this autoantibody is associated with pregnancy.
Key words: Autoantibody, Antiphospholipid syndrome, Recurrent Miscarriage
A Ebrahimzadeh, S Mohammadi, T Davoodi, Ar Salimi Khorashad, A Jamshidi,
Volume 7, Issue 3 (10-2013)
Abstract
Abstract
Background and Objective: Toxoplasmosis is one of the most prevalent parasitic infections worldwide. Contamination of pregnant women with toxoplasmosis may cause fetal death, preterm delivery and congenital toxoplasmosis. Due to importance of congenital Toxoplasmosis and the need of further study, this research was accomplished in Zahedan.
Material and Methods: The serum samples (N= 221) were collected from pregnant women referring to reference laboratory of Zahedan in 2011. The IgG and IgM antibody levels against toxoplasmosis were investigated using ELISA method.
Results: Out of all samples, 30.8% are IgG positive and 1.4% are both IgG and IgM positive. There is no significant difference between positive and negative groups using Chi-square tests.
Conclusion: The main part of pregnant women in Zahedan (69.2%) is serologically negative against toxoplasmosis therefore, hygiene education to eliminate risk factors especially during pregnancy period seems to be imperative.
Keywords: ELISA Antibody Pregnancy Toxoplasma Zahedan
Mj Gharavi, M Roozbehani, Ah Ajodani, S Yosefi Darestani,
Volume 7, Issue 4 (1-2014)
Abstract
Abstract
Background and Objective: The patients with Post-Infectious Irritable bowel syndrome (PI-IBS), a subtype of irritable bowel syndrome, suffer from bacterial gastroenteritis. Since campylobacter Jejuni (CJ) is one of the most common agents in this syndrome, we aimed to evaluate the prevalence of Campylobacter Jejuni in patients with Irritable Bowel Disease.
Material and Methods: This case - control study was conducted on 160 patients divided into 2 equal groups of healthy and unhealthy. The presence of Anti- CJ antibody (IgG and IgA) was evaluated by ELISA and the comparison was performed by chi-square test.
Results: The mean age of case (31.51) and control (31.84) was not statistically different (P = 0.87). Titer of Anti- Campylobacter Jejuni antibody IgG was positive in 25% of patients and 18.8% of the healthy ones (p=0.02). IgA Seropositivity in patients was 7.5% but no one in control group was positive (p =0.01)
Conclusion: Seroprevalence of Cj in patients with IBS was higher significantly than that of control group. Thus, Cj can be known as one of the causes of Post-infection in patients with IBS in our region and it should be paid more attention in diagnostic assessment of these patients.
Keyword: Irritable Bowel Syndrome Campylobacter Jejuni Antibody
Sh Semnani, Gh Roshandel, H Faghih, F Ghasemi Kebria, H Joshghani,
Volume 7, Issue 4 (1-2014)
Abstract
Abstract
Background and Objective: The patients with Post-Infectious Irritable bowel syndrome (PI-IBS), a subtype of irritable bowel syndrome, suffer from bacterial gastroenteritis. Since campylobacter Jejuni (CJ) is one of the most common agents in this syndrome, we aimed to evaluate the prevalence of Campylobacter Jejuni in patients with Irritable Bowel Disease.
Material and Methods: This case - control study was conducted on 160 patients divided into 2 equal groups of healthy and unhealthy. The presence of Anti- CJ antibody (IgG and IgA) was evaluated by ELISA and the comparison was performed by chi-square test.
Results: The mean age of case (31.51) and control (31.84) was not statistically different (P = 0.87). Titer of Anti- Campylobacter Jejuni antibody IgG was positive in 25% of patients and 18.8% of the healthy ones (p=0.02). IgA Seropositivity in patients was 7.5% but no one in control group was positive (p =0.01)
Conclusion: Seroprevalence of Cj in patients with IBS was higher significantly than that of control group. Thus, Cj can be known as one of the causes of Post-infection in patients with IBS in our region and it should be paid more attention in diagnostic assessment of these patients.
Keyword: Irritable Bowel Syndrome Campylobacter Jejuni Antibody
Gharavi, Mj, Roozbehani, M, Bakhshkar, H,
Volume 8, Issue 5 (1-2015)
Abstract
Abstract Background and Objectives: Giardia lamblia and Entamoeba histolytica are the most prevalent human intestinal pathogenic protozoa, worldwide. The clinical features of Giardia infection are acute diarrhea, a chronic condition with continuous diarrhea and malabsorption. Entamoeba histolytica invade intestinal tract without any typical clinical indications, and it can involve liver and other organs too. Therefore, we aimed to study these protozoa by serological and parasitological methods. Material and Methods: In this comparative study, the stool and blood specimens were collected from 1025 patients selected via simple random sampling in three different laboratories located in Tehran and Karaj, Iran (2012). Formalin Detergent test was performed on all samples. Both serum and stool positive samples of this method were analyzed for antigen and antibodies related to Giardia lamblia and Entamoeba histolytica, respectively. Results: of 1025 stool specimens, 76 (4.7%) were positive for Giardia lamblia and 19 (1.8%) for Entamoeba histolytica using Formalin-detergent method. In ELISA, 81 (7.9%) coproantibodies to Giardia lamblia and 24 (2.3%) coproantibodies to Entamoeba histolytica, 78 (7.6%) corproantigen for Giardia lamblia, and 5 (0.4%) for Entamoeba histolytica were observed. circulatory antibodies to Entamoeba histlytica were detected in 22 cases (2.1%) Conclusion: Sensitivity of microscopic method compared to serological methods is higher than 90% therefore, Formalin-detergent method can be the best method for stool examination. Key words: Giardia Lamblia and Entamoeba Histolytica, Coproantibody, Coproantigen, Blood Antibody
Y Shamsizadeh , F Roodbari , N Arbab Soleymani ,
Volume 9, Issue 3 (9-2015)
Abstract
Abstract
Background and Objective: West Nile virus (WNV) is a member of the genus Flavivirus that can cause viral infections in human. This study aimed at detecting IgG antibodies against WNV in patients of two cities of Neka and Shiraz.
Material and Methods: the participants were 46 possible WNV case from Neka (13 women and 10 men) and Shiraz (10 women and 13 men). IgG assay was carried out using the Elisa method.
Results: Immune Status Ratio (ISR) in Neka was negative for WNV IgG, but 12 from Shiraz, including 2 women and 10 men, were positive for WNV IgG that was changed from 3.12 to 38.6. Considering ISR, there was significant variation between Neka and Shiraz (p<0.05). In addition, results showed that there was significant variation in WNV infection rate between men (39.19%) and women (13.04%) from Neka and Shiraz cities (p<0.05).
Conclusion: Because Shiraz has hot and semi-dry climate, whereas Neka temperate climate, the results were affected by climate variation. Given the outdoor job of men compared to women, they are exposed to the bite of mosquito vectors that transmit WNV.
Keywords: West Nile Virus; Elisa; IgG Antibody; Iran.
Haleh Foroutan , Marziyeh Khodabakhsh , Masoud Moharamzadeh ,
Volume 10, Issue 4 (7-2016)
Abstract
ABSTRACT
Background and Objective: Prostate specific antigen (PSA) is considered as one of the most reliable biomarkers of cancer and other known prostate diseases. In the present study, solid phase sandwich immunoradiometric assay was used to measure the amount of PSA. In this type of measurement, a pair of anti-PSA antibodies on the solid phase and labeled with Iodine-125, participate in forming a complex with two different epitopes of PSA.
Methods: Variables such as irradiation level, modification of polymer surfaces by alcohol washing, different concentrations and volumes of antibody, incubation temperature and drying conditions that influence the direct coating process were optimized. Finally, the stability, accuracy and precision of the laboratory kit were evaluated by comparison with a foreign kit.
Results: According to the obtained results, preliminary preparations such as irradiation, tube washing and specific temperature conditions are not required during the coating process. Drying by lyophilization method does not affect the quality of coating. Antibody concentration of 2.5 μg/ml and coating volume of 800 μl were determined as the optimum conditions for coating, which had good stability within a year. Alignment of results obtained from the domestic and foreign kits for accuracy of 30 samples from patients was confirmed by T-test (sig 2-tailed= 0.993 and 95% confidence interval). The short-term and long-term precision for three control ranges (low, medium, high) were less than 0.25 and 0.33 of allowable total error (TEa = 10%), respectively.
Conclusion: The produced domestic kit has acceptable precision according to the CLIA criteria.
Keywords: Biological testing, Radioimmunometric assay, monoclonal antibody, prostate specific antigen, prostate disease.
Sina Soleimani , Morteza Kamalzadeh , Mohsen Lotfi ,
Volume 13, Issue 1 (1-2019)
Abstract
ABSTRACT
Background and Objectives: Poliomyelitis remains a major public health problem in developing countries, which signify the need for extensive diagnostic and prevention research. The aim of the present study was to design monoclonal antibodies (MAbs) against poliovirus type I with biomedical, diagnostic and therapeutic applications.
Methods: B-cells were isolated from a mouse challenged with polio antigen injection. The B-cell were fused with myeloma tumor cells. After evaluation and screening of approximately 250 hybridoma colons by ELISA, 35 colons with the highest antibody titer and no cross-reactivity were selected and subsequently cloned by limiting dilution. Finally, three colons capable of secreting MAbs against epitopes of poliovirus type I were used for MAb production. Next, the MAbs were characterized by antibody assays, isotyping, epitope analysis (western blot), cross-reactivity test, stability test, sterility test and mycoplasma test.
Results: The results indicated that the MAbs were of IgG1 kappa chain, had good stability and no cross-reactivity. In western blot, a band at 26 kDa which is associated to VP3 neutralization protein was observed.
Conclusion: These serotype-specific MAbs can be potentially used for identification of type I poliovirus for research, diagnostic and prevention purposes.
Keywords: Monoclonal antibody, Hybridoma, Poliomyelitis, Poliovirus.
