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Showing 3 results for Angiogenesis
Designing and Construction of Recombinant Plasmid Consisting of Basic Fibroblast Growth Factor and Immunodominant Fragments of Pseudomonas Exotoxin
H Haghighatfard, Y Yazdani, Y.,
Volume 9, Issue 1 (4-2015)
Abstract
Abstract Background and Objective: the inhibition of tumor-associated angiogenesis can significantly reduce the tumor proliferation. The basic fibroblast growth factor (bFGF), an important angiogenic factor, is considered as a potential therapeutic target for cancer therapy. The purpose of this study was evaluating, designing and construction of new recombinant DNA molecule in order to have efficient expression of a fusion protein consisting of the bFGF and immunodominant epitopes of Pseudomonas toxin. Material and Methods: Different types of peptide linker, codon adaptation index (CAI) and adding signal peptide were considered in designing of immunogenic coding sequence. After software evaluation, the recombinant DNA molecule was ordered in the puc57 cloning vector. Then, coding sequence inserted into the multiple cloning site of pET28-a plasmid. Finally, PCR and enzymatic digestion tests were done for evaluation of recombinant expression vector. Results: Optimization of DNA sequence, codon adaptation index (CAI) increased from 0.69 to 0.83 and GC content decreased from 61 to 54.77. The presence of 1214-bp PCR product and 1029-bp one obtaining from enzymatic digestion confirmed the correction of the cloning process. Conclusion: According to the previous studies, it is the first work for designing, optimizing and synthesis of recombinant DNA consisting of bFGF and immunodominant epitopes of Pseudomonas toxin. Keywords: Tumor angiogenesis, immunodominant epitopres of Pseudomonas toxin, Fibroblast growth factor 2, DNA 2 software
Impact of Aerobic Exercise on Restoration of Soleus and Gastrocnemius Muscles Microcirculations in Wistar Rats with Chronic Heart Failure
Farzad Nazem, Malihe Ardakani, Mohammad Reza Gholami, Kamal Ranjbar, Afshin Nazari,
Volume 10, Issue 5 (9-2016)
Abstract

ABSTRACT

        Background and Objective: Scientific evidence reveals that the density of skeletal muscle microcirculations decreases in the patients with chronic heart failure. Therefore, this study aimed to determine the impact of submaximal aerobic exercise training on restoration of fast/slow-twitch muscle fibers microcirculation in rats with myocardial infarction.

         Methods: In this experimental study, 30 male infarcted Wistar rats were randomly divided into three groups of sham (N=10), control (N=10) and exercise (N=10), four weeks after surgery (blockage of anterior descending artery). Daily aerobic exercise included running on a treadmill for 10 weeks (5 times a week) at a speed of 17 m/min within 10 to 50 minutes. The animals were anesthetized and then sacrificed with chloroform, 48 hours after the last training session. The microcirculation density of soleus (SOL) and gastrocnemius (Gw) muscles was evaluated by immunohistochemistry assay.

        Results: Capillary density and capillary to SOL muscle fiber ratio significantly increased in response to exercise training. However, capillary density, capillary to Gw muscle fiber ratio and Gw muscle arteriolar density of Wistar rats with chronic heart failure were not significantly affected by the exercise program. Moreover, arteriolar density of Gw muscle increased significantly after the exercise program.

        Conclusion: The ten-week aerobic exercise might non-uniformly improve the SOL and Gw muscles microcirculation in rats with chronic heart failure by increasing capillary density and capillary to fiber ratio in the SOL muscle and increasing the arteriolar density of the Gw muscle.

        Keywords: Heart Failure, Angiogenesis, Exercise.


The Role of Angiogenesis in Colorectal Polyps and Cancer, a Review
Hamid Asadzadeh Aghdaei , Zahra Pezeshkian, Meghdad Abdollahpour-Alitappeh , Ehsan Nazemalhosseini Mojarad , Mohammad Reza Zali,
Volume 12, Issue 4 (7-2018)
Abstract
ABSTRACT
          Colorectal cancer remains a leading cause of morbidity and mortality worldwide. Most colorectal cancers begin as a growth of tissue called a polyp, some types of which can change into cancer over the course of several years. Angiogenesis, the formation of new blood vessels, is known to play a key role in the initiation, growth and progression of polyp and colorectal cancer. In this review, we discuss the mechanisms involved in colorectal cancer and polyp angiogenesis.
          Keywords: Colorectal Neoplasms, Polyps, Angiogenesis
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