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Showing 2 results for Lactate Dehydrogenase
Evaluation of LDH Activity and Its Relationship with Fructose Levels in Seminal Plasma of Normospermic and Asthenospermic Males
Mohammad Mohammad Mostakhdem Hashemi , Afsaneh Tabandeh , Hamidreza Tajari , Nasser Behnampour, Azadeh Aliarab, Hamid Reza Joshaghani,
Volume 10, Issue 4 (7-2016)
Abstract

ABSTRACT

        Background and Objectives: sperm motility mainly depends on aerobic glycolysis, and lactate dehydrogenase (LDH) is a key enzyme in the last step of this process. On the other hand, fructose is considered as the main energy source for sperm motility. Therefore, this study aimed to evaluate the LDH activity and fructose levels and their correlation with sperm motion parameters in normospermic and asthenozospermic males.

       Methods: In this case-control study, 96 normospermic and 96 asthenozospermic males were enrolled.  The semen samples were analyzed by computer assisted semen analysis to identify sperm motion parameters. Seminal plasma fructose levels were measured using Seliwanoff's test. LDH activity in the samples was evaluated using commercially available kits. The obtained data were analyzed by SPSS software (version 16).

       Results: The levels of LDH activity and fructose concentration were not different between the two groups. There was no correlation found between LDH activity and fructose concentration or sperm motility parameters. However, LDH was significantly correlated with sperm concentration in both groups.

      Conclusion: Although LDH activity and fructose levels seem to be influenced by sperm motility, other factors such as sperm concentration and total sperm count can also affect their seminal plasma levels. Thus, when the sperm concentrations are equal in study groups, the level of these factors are expected to be equal.

         Keywords: Lactate Dehydrogenase, Fructose, Sperm Motility, CASA.


Predicting the severity of sickle cell disease using hematological, biochemical, and cellular parameters
Viren Laljbhai Vaghasiya , Divya D Bambhaniya , Jitendra Gordhanbhai Nasit , Bhoomika H Rupavatiya ,
Volume 20, Issue 1 (1-2026)
Abstract
Background: Sickle cell disease is a hemoglobinopathy caused by a point mutation and has a heterogeneous clinical course. The level of Hb F within erythrocytes is believed to be the most important parameter determining disease severity. The aim of this study was to investigate whether Hb F level, F-cell count, and sickle cell percentage after in vitro induction of sickling can predict the severity of the disease.
Methods: All necessary data were collected from clinical history, biochemistry, and pathology laboratory tests. This was a cross-sectional study with 31 participants. Statistical analyses were performed using the correlation coefficient and chi-square test to identify significant differences between variables. Statistical analysis was performed using MedCalc software.
Results: The majority of patients fell into the mild severity score category, with a lack of severe disease phenotypes. The number of painful episodes, hospitalizations, and cumulative disease severity scores were associated with high levels of LDH and indirect bilirubin. However, none of the clinical disease severity parameters or the overall cumulative disease severity score was associated with Hb F level, F-cell count, or the percentage of sickled cells after in vitro induction of sickling. A high percentage of F-cells was associated with high MCV, MCH, and MCHC and low RDW, LDH, and indirect bilirubin levels.
Conclusion: Sickle cell disease severity is related to the susceptibility of RBCs to hemolysis, as indicated by serum LDH and indirect bilirubin levels. However, the extent of hemolysis may depend on multiple factors rather than F-cell count or Hb F level alone.

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