Showing 11 results for Sharifi
Mahsa Yazdi, Ali Nazemi, Mir Saed Mir Nargasi, Mr Khataminejad, Sh Sharifi, M Babai Kochkaksaraei,
Volume 4, Issue 1 (Spring - Summer 2010[PERSIAN] 2010)
Abstract
Abstract Background and objectives: Beta-lactamase enzymes are the most causes of resistance to antibiotics among gram-negative bacteria. Nowadays, Infections due to ESBLs are being increased throughout the world and is considered as a new burden to the health systems. This study aimed at determining the sensitivity pattern of E.coli isolates to beta-lactam antibiotics, and investigating the presence of blaCTX-M, blaTEM, and blaSHV genes in the urine samples. . Material and Methods: In this study, 244 E.coli isolates were screened in 2009-2010. The antibiotic susceptibility of E. coli isolates were determined by disc-diffusion method. Antimicrobial agents tested were cefoxatime, ceftazidime, imipenem, nalidixic acid, and ciprofloxacin. The combined disc test was used to confirm the results. The results were compared to the Clinical and Laboratory Standards Institute (CLSI) and ESBL positive isolates were further investigated for the presence of blaCTX-M, blaTEM, and blaSHV genes by PCR. Results: Of 244 E. coli isolates, 116 (47.1%) are resistant to Ceftazidime, and 96 (39.2%) to cefoxatime. Also, 109 (44.3%) isolates are ESBL positive. blaCTX-M, blaTEM, and blaSHV genes are found among 95 (87.1%), 75 (68.8%), and 77 (70.6%) ESBL positive isolates, respectively. Forty (36.6%) isolates have all three genes, while 68 (62.3%) include blaTEM and blaSHV genes. Moreover, 61 (55.9%) isolates carry blaCTX-M and blaTEM genes, and 54(49.5%) have blactx-M and blashv. Conclusion: Regarding the high frequency of resistance to the third generation cephalosporin antibiotics, precise antibiogram testing is highly recommended before any antibiotic prescription in cases of infections with ESBL producing microorganisms. Key words: ESBL Escherichia coli blaCTX-M blaTEM blaSHV.
A Nazemi, M Naderi, M Jafarpour, M Mirinargesi, Sh Sharifi,
Volume 4, Issue 2 (Autumn – Winter 2011[PERSIAN] 2010)
Abstract
Abstract Bachground and objectives: The ability of adherence to the surface of host cell is very critical in the colonization of microbial pathogens. It has been revealed that E. coli strains that infect urinary tracts have different fimbrea such as I, S, FIC, Dr, and fimbrial adhesions. Material and Methods: In this study, 363 urine samples were obtained from patients with urinary tract infections reffered to clinical laboratories in Western areas of Tehran ,2008-2010 by using biochemical tests,200 samples were confirmed to be E.coli.First, DNA was extracted by boiling method and then the presence of fimbria fim, sfa, pap, foc, and afa genes tested by PCR. Results: In 200 samples, the frequency of fimbria fim, sfa, pap, foc, and afa genes are188 (%94 ), 34 (%17), 20 (%10), 61 (%31) and 71 (%35.5), respectively. Conclusion: The resultes show that FIM ans SFA are the most fimbrial genes of E. coli isolated from urine samples .This information can be valuable in etiology of urinary tract infection (UTI), UTI administration, and making of new vaccines. Key words: Urinary tract infection, fimbria, Uropathogenic E. coli (UPEC)
Soltan Dallal. M.m, Rahimi Forushani, A., Sadigh Maroufi, S, Sharifi Yazdi, K,
Volume 5, Issue 2 (Autumn – Winter 2011[PERSIAN] 2011)
Abstract
Abstract Bachground and objectives: Salmonella is one of the most important agents of gastrointestinal infection and diarrhea in our country. Misdiagnosis of these bacteria leads to cure failure. The aim of this study was to make a comparison between PCR and the API-20E and conventional biochemical tests carried out for the identification of Salmonella. Material and Methods: In this study 470 specimens taken from children, with acute gastroenteritis, referred to teaching hospitals called Imam, Shariati and children medical centre. The specimens were transferred to microbiology laboratory in public health school for identification of Salmonella with PCR and API-20E methods. Results: Of 470 specimens, 65(13.8%) are positive for salmonella in hospital laboratory, while 37 (7.9%) for API-20E and 39 (8.3%) for PCR are positive. The results of antibiotic sensitivity tests on 39 salmonella isolated from diarrhea specimens show that 73.3% of them are resistance to at least one of the sixteen antibiotics tested. Conclusion: Based on the the results, there is significant difference (P<0.05) between conventional method, API-20E and PCR Key words: Salmonella, conventional identification, molecular identification
Mm Soltan Dallal, A Rahimi Forushani, K Sharifi Yazdi, B Nikmanesh, A Rastegar Lari,, A Aminharati,
Volume 7, Issue 1 (spring[PERSIAN] 2013)
Abstract
Abstract
Background and Objectives: gasterointertidis is one of the most common forms of Salmonellosis, which is a worldwide problem. The invasive characteristic of intestinal bacteria is one of their pathogenicity Mechanisms , which can be easily investigated by cell culture technique. In this study ,the invasive characteristic of some Salmonella serogroup were investigated by using HEP-2 cell.
Methods and Material: The rectals soap were prepared from 280 diarrhea patients referred to Imam Khomeyni and children medical centres , 140 with bloody diarrhea and 140 with watery diarrhea as a comparison group. The rectal soap was taken before patients taking any antibiotics, and 140 rectal specimens were taken from healthy people as a control group. All the samples were inoculated in differential and selective media, like Hektoen enteric agar and Xylose lysine deoxycholate (XLD) agar .After incubation at 37C for 24 hours, the colonies were examined and identified by conventional biochemical and serological tests. Using HEP-2, cellular invasion characteristic of Salmonella serogroups was assessed. Moreover, the antibiotic resistance patterns were performed according to Clinical and Laboratory Standards Institute (CLSI).
Results: Of all tested samples, 35(8.3%) are Salmonella strains. The frequency of Salmonella is reported for bloody diarrhea (5.2%) , watery diarrhea ( 1.7%) and control group( 1.4%) .The most abundant serogroups with invasive characteristic, using HEP-2 cell culture, are serogroup B ( 62.9%) and D (17.2%).
Conclusion
The results obtained in this study show that the majority of Salmonella isolates are without invasive characteristic.
Key words: Salmonella, Diarrhea, Cell invasion, Cell culture
A Sharifi, N Razmi, N Naghsh,
Volume 7, Issue 4 (winter[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: Flavonoids play an important role in non-enzymatic reaction against oxidative stress. These are polyphenolic compounds in tea structure that could be reacted with free radicals and neutralized them. In this study, we investigated the anti-oxidant impact of Camellia Sinesis on the liver of thioacetamide -injected male albino mice.
Material and Methods: In this study, 40 male mice were categorized in five groups of eight. The first group was control. The second and the third group received 100mg/kg and 150mg/kg of thioacetamide, respectively. The fourth group received 100mg/kg thioacetamide followed by black tea (5 gr/100) and the fifth one received 150mg/kg thioacetamide followed by black tea (5 gr/100). Tioacetamide was given via intraperitoneal. After that, for 30 days, they were only fed on black tea (5 gr/100). At the end, catalase (CAT) and glutathione peroxidase (GPx) activity were measured.
Results: Based on the results, catalase(CAT) and glutathione peroxidase(GPx) activity were significantly increased in the groups of Thioacetamide and black tea compared to those of only Thioacetamide groups (p<0.05).
Conclusion: The increase of these enzymes in tea groups shows the anti-oxidant effect of black tea that can be caused by Catechin.
Keywords: Antioxidant Thioacetamide Black Tea Glutathione Peroxidase Catalase
Hesam Alizade , Fatemeh Fallah , Reza Ghanbarpour , Hosein Goudarzi , Hamid Sharifi , Mohammad Reza Aflatoonian ,
Volume 10, Issue 2 (Mar,Apr2016 2016)
Abstract
ABSTRACT
Background and Objective: One of the main tasks of clinical microbiology laboratories is to determine antibiotic resistance profiles in common pathogens and ensure the selection of effective antibiotics for certain infections. The aim of this study was to compare the methods of disk diffusion, broth microdilution and modified Hodge test in Escherichia coli isolates from urinary tract infection and diarrhea for susceptibility testing against beta-lactam antibiotics in Kerman, Iran.
Methods: In this study, 432 E. coli isolates were collected from diarrhea (216 isolates) and urinary tract infection samples (216 isolates). The antibiotic susceptibility testing methods of disk diffusion, broth microdilution and modified Hodge test were performed according to the Clinical and Laboratory Standards Institute guidelines.
Results: The findings of disk diffusion method showed that resistance to cefotaxime, ceftazidime, aztreonam, cefepime and imipenem was 51.15%, 30.55%, 24.30%, 15.27% and 1.85%, respectively. In the disk diffusion test, 51.15% of the isolates were resistant to at least one antibiotic, all of which were later evaluated by the broth microdilution method. Moreover, 52.94%, 17.19%, 13.12% and 0.90% of the isolates were resistant to cefotaxime, ceftazidime, cefepime and imipenem, respectively. All of the isolates were evaluated for the production of carbapenemase enzyme by the modified Hodge test and none of the isolates were found as positive.
Conclusion: This study shows that performing carbapenem tests is very challenging, and laboratories are recommended to use secondary and independent antibiotic susceptibility tests such as modified Hodge test to confirm the carbapenem-resistant results.
Abbass Ghanbari-Niaki , Fatemeh Islami, Ramezanali Arabameri, Aliakbar Sharifiyan,
Volume 11, Issue 5 (Sep - Oct 2017)
Abstract
ABSTRACT
Background and Objectives: The aim of the present study was to investigate the effect of six weeks of volleyball training on lipid and lipoprotein profiles of elite high school male volleyball players in the Golestan Province (Iran) during preparation season for an international championship in France.
Methods: Training group included 12 elite volleyball players in students’ premier league and control group included 12 healthy non-athlete male students. The training group practiced at 60-85% of maximum heart rate for six weeks, 90 minutes per session, six sessions per week and three sessions a day (first session: technical practice, second session: tactical practice and third session: technical/tactical practice). Blood samples were taken after 12 hours of fasting both before and after the training.
Results: Level of total cholesterol (TC), high-density lipoprotein-cholesterol (HDL-C), low-density lipoprotein-cholesterol (LDL-C) and LDL-C/HDL-C increased in the training group (P> 0.05). However, there was a significant difference between the two groups in TC and LDL-C levels (P<0.05). Level of triglyceride, VLDL, and TC/HDL-C reduced in the training group after six weeks of training (P> 0.05).
Conclusion: Our results show that the six-week volleyball training during the preparation season can cause abnormalities in plasma lipid profile.
Keywords: Lipid, Lipoprotein, Volleyball Player
Esmaeil Khorshid Sofyani, Rasoul Sharifi,
Volume 14, Issue 4 (Jul-Aug 2020)
Abstract
Background and objectives: Combination chemotherapy with new adjuvants has been introduced as an innovative method of treating various types of cancer. The aim of this study was to investigate potential synergistic effect of quinacrine on the anti-proliferative and anti-apoptotic activity of docetaxel in
A549 lung cancer cells.
Methods: Cell viability and apoptosis percentage were evaluated with MTT assay and annexin V staining. To understand the mechanisms through which quinacrine modulates expression of pro-apoptotic and anti-apoptotic genes, expression of Bcl-2, Bcl-xl and Bax genes were investigated using
real-time RT-PCR.
Results: The half maximal inhibitory concentration values for docetaxel and quinacrine was 3.16±1.5 nM and 4.4±0.58 μM, respectively. The combination index value of docetaxel and quinacrine was 0.66 against A549 cells, indicating strong synergism. The expression of anti-apoptotic genes Bcl-2 and Bcl-xl reduced significantly, while the expression of the pro-apoptotic gene Bax increased significantly after co-treatment with docetaxel and quinacrine (P<0.05). Treatment of cells with a combination of quinacrine and docetaxel significantly increased the inhibitory effect of docetaxel (reduced proliferation by 50%) and the percentage of apoptotic cells.
Conclusion: Our findings suggest that the combination of quinacrine and docetaxel can be considered as a promising strategy for the treatment of patients with lung cancer.
Abdolrasoul Hakim Elahi, Rasoul Sharifi, Minoo Mahmoodi, Seyed Mehrdad Kassaee,
Volume 14, Issue 5 (Sep-Oct 2020)
Abstract
Background and objectives: The aim of this study was to evaluate the effectiveness of octopine (phytogenic-derivative of arginine) on antioxidant indices, trace elements and lipid profiles of a mouse model of breast cancer.
Methods: In this study, 48 Balb/c mice were divided into six groups: healthy control, cancer control, cancer group receiving 50 mg of octopine, cancer group receiving 100 mg of octopine and cancer group receiving 150 mg of octopine. The octopine treatment was carried out for three weeks. The 4T1 cell line was used to induce cancer. Fasting blood samples were taken from mice to evaluate lipid profile, copper and zinc levels. Malondialdehyde, superoxide dismutase and glutathione peroxidase activity in breast tumor tissues was evaluated. Data were analyzed by SPSS 18 software using one-way ANOVA and t-test.
Results: Octopine had no significant effect on superoxide dismutase and glutathione peroxidase activity in the treatment group compared with the control cancer group. However, it significantly increased total antioxidant capacity and decreased malondialdehyde activities. Furthermore, treatment with octopine significantly decreased serum zinc, copper, TG, cholesterol and low-density lipoprotein levels but significantly increased high-density lipoprotein compared with the untreated cancer group.
Conclusion: Octopine administration is effective in reducing some oxidative stress indices and improving trace elements abnormalities and lipid profile in mouse models of breast cancer.
Afieh Samimi, Oghol Niaz Jorjani, Zohreh Sharifi, Faramarz Koohsar, Khodaberdi Kalavi, Fatemeh Mesgarian, Beniamin Talebi ,
Volume 16, Issue 3 (May-Jun 2022)
Abstract
Background and objectives: Cutaneous leishmaniasis is endemic in most areas of Iran, and the diagnosis of its species is essential for controlling the disease. Leishmania major is the causative agent for cutaneous leishmaniasis in humans. Molecular methods are generally more sensitive than microscopic methods. The present study aimed to use a polymerase chain reaction-enzyme linked immunosorbent assay (PCR-ELISA) technique for detecting live L. major from wounds of patients with cutaneous leishmaniasis.
Methods: In the present study, a standard strain of L. major promastigotes was used as the positive control for purification of DNA. The Novy–MacNeal–Nicolle and RPMI-1640 media were used for reproduction of parasites. DNA was isolated from specimens taken from 35 patients with suspected cutaneous leishmaniasis whose disease was confirmed by direct smear method. The PCR-ELISA technique was later applied by using the standard strain, patient specimens, and primers specific for the 18s rRNA.
Results: Out of 35 patients, 17 (48.6%) were male and 18 (51.4%) were female. In addition, 8.6% of the patients lived in the Gonbad-e Kavus County, while all patients had been infected in villages around Gonbad-e Kavus. Of 35 patients with confirmed cutaneous leishmaniasis according to the direct smear method, 31 patients (86.31%) had leishmaniasis based on the PCR method and the PCR-ELISA methods.
Conclusion: Based on the results, the PCR-ELISA method is more sensitive and accurate for detecting L. major.
Arvin Shajeie, Mehrnaz Rad, Mahdi Askari, Kamran Sharifi, Gholamreza Hashemi Tabar,
Volume 17, Issue 5 (Sep-Oct 2023)
Abstract
Background: Colistin is the most significant last-line antibiotic for the treatment of multidrug-resistant infections caused by Gram-negative bacteria, especially the Enterobacteriaceae family. The emergence and rapid spread of the plasmid-mediated resistance gene, mcr-1 (mobilized colistin resistance), in some isolates of Escherichia coli in recent years provoked public health concerns since it has been shown that mcr-1 with other resistance genes, such as ESBLs (extended-spectrum beta-lactamases) and carbapenemases, could be carried on a single plasmid concurrently. The excessive consumption of colistin, particularly in the livestock industry, and the transmission of these resistant bacteria from livestock to humans may potentially increase the risk of the spread of resistance in humans. Therefore, this study aimed to detect the prevalence of mcr and carbapenem resistance genes among neonatal calves in Mashhad, Razavi Khorasan Province, Iran.
Methods: In the current study, 200 fecal samples from healthy and diarrheic neonatal calves (≤35 days old) were collected in Mashhad (190 E. coli strains were isolated). Antibiotic susceptibility to ceftazidime, cefepime, cefixime, meropenem, colistin, and ciprofloxacin was examined. The double-disk diffusion method (ceftazidime + ceftazidime/clavulanic acid) was performed on Mueller-Hinton agar (MHA) media to phenotypically distinguish the ESBL producers. Afterward, the Multiplex polymerase chain reaction (PCR) method was used to detect colistin resistance genes (mcr-1, mcr-2, mcr-3, mcr-4, and mcr5), NDM-1 (New Delhi metallo-beta-lactamase 1), and OXA-48 as carbapenemases.
Results: The results of the resistance rate to antibiotics were cefepime, ceftazidime, cefixime, meropenem, and colistin. Based on the findings, 33.7% were phenotypically ESBL producers, 4.21% harbored mcr-1, and no NDM-1 or OXA-48 was detected. Among the mcr-1-positive isolates, 5 strains showed the ESBL phenotype.
Conclusion: The results highlight the need for continued monitoring of antibiotic resistance in livestock and the potential for transmission to humans. The findings also underscore the importance of responsible antibiotic use in both human and animal health to mitigate the spread of antibiotic resistance.
