Mahsa Aghaei, Dr. Leila Asadpour, Dr. Amir Arasteh,
Volume 18, Issue 4 (7-2024)
Abstract
Background and Objectives. Staphylococcus aureus is one of the most common causing agent of nosocomial infection, worldwide. The methicillin-resistant and biofilm-dependent infections of this bacterium has become a clinical concern in patients. This research aimed to identify biofilm forming ability and agr- specific group of clinical isolates of methicillin-resistant S. aureus (MRSA) in northern Iran.
Methods: During 2021, a total of 200 clinical isolates were identified as S. aureus by biochemical tests. The disk diffusion method was used to examine the antibiotic resistance of isolates and the microplate method was applied to investigate the biofilm production capability. In addition, the PCR method was used to determine the frequency of biofilm associated genes and agr typing of MRSA isolates. P£0.05 was considered significant.
Results: Overall, 125 (62.5%) out of 200 isolates were methicillin-resistant and 75% were multiple antibiotic-resistant. Vancomycin was the most efficient antibiotic against the isolates and highest resistant rate was against penicillin. Biofilm forming ability was detected in 99 (79.2%) methicillin-resistant isolates in which icaA and icaD were found in 85% and 78% of biofilm-producing isolates, respectively. Type 1 of the agr gene was the most common type among methicillin-resistant isolates. The frequency of biofilm associated genes showed significant association with MDR phenotype and presence of agr locus (P£0.05).
Conclusion: Present results indicate high frequency of biofilm and antibacterial resistance in methicillin-resistant S. aureus isolates in Guilan Province, northern Iran. Present data suggests reliable and rapid identification of biofilm forming MRSA strains to prevent the spread of these bacteria.
