Search published articles


Showing 2 results for Zarei
Frequency of Bacterial Frequency of Bacterial Contamination in Traditional Ice Cream Produced in Arak, Iran (2011)
M Rezaei, R Ghasemi Khah, M Parviz, O Zarei,
Volume 8, Issue 2 (summer 2014[PERSIAN] 2014)
Abstract
Abstract Background and Objective: Ice cream is a suitable environment for microbial growth due to its chemical structure, ingredients, and its increased supply and demand. In the absence of hygienic considerations, it can cause poisoning. This study aimed to determine bacterial contamination in traditional ice cream produced in Arak city in 2011. Material and Methods: The samples (n= 30) were randomly obtained from different parts of Arak in, 2011. The Samples were shipped in cold conditions and total count of microorganisms test was performed according to Iranian national standards. Results: In 16.66%, the microbial contamination was below the limit of microbial load (5×104), and in 83.3% the contamination was more than allowed level. Conclusion: This study highlights the dire situation for bacterial contamination of traditional ice cream in Arak city. Keywords: Arak, Ice Cream, Microbial Contamination
Prevalence of Virulence Genes of Escherichia Coli O157:H7 Isolated from Patients with Urinary Tract Infections in Shiraz, Iran
Kargar, M, Kargar, M, Zareian Jahromi, M,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract

Abstract

Background and Objective: Escherichia coli O157:H7 is one of the most well-known pathogenic bacteria worldwide that can develop severe diseases such as hemolytic uremic syndrome (HUS). This study aimed to assess the prevalence of virulence genes of E. coli O157:H7 in patients with suspected urinary tract infections (UTIs).

Material and Methods: This cross-sectional study was conducted on 10,372 urine samples collected from patients with suspected UTI from six hospitals and clinical laboratories in Shiraz city. CT-SMAC medium, b-glucosidase activity test (MUG), specific antiserum, and the presence of O157 and H7 genes by PCR were used to confirm E. coli O157:H7 isolates. Then, stx1, stx2, eaeA, and hlyA genes were evaluated using multiplex PCR.

Results: In this study, 16 (7.8%) and 13 (6.3%) bacteria had O157 and H7 genes, respectively. Evaluation of virulence genes showed that genes eaeA (15.4%), stx1 and eaeA (15.4%), stx2 (7.7%), and stx2 and eaeA (7.7%) had the highest frequency in E. coli O157:H7.

Conclusion: Due to the severity of pathogenicity, low infectious dose of E. coli O157: H7, and its pathogenic genes, more extensive studies and genotyping of E. coli O157: H7 are required to be conducted in other areas of Iran in order to measure the frequency in UTIs and control the infections caused by E. coli O157: H7.

Keywords: Escherichia coli O157:H7; Urinary Tract Infections; Shiga Toxin 1; Shiga Toxin 2.


Page 1 from 1     

© 2007 All Rights Reserved | Medical Laboratory Journal

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.