Ameneh Elikaei , Hossein Vazini, Fatemeh Javani Jouni , Jaber Zafari,
Volume 13, Issue 5 (Sep-Oct 2019)
Abstract
ABSTRACT
Background and objectives: Esophageal cancer is the eighth most common type of cancer in the world. Considering the adverse effects of anticancer drugs and the emergence of chemotherapy resistance, plant-derived extracts and their constituents could be a valuable source of novel anticancer drugs. In this study, we investigated cytotoxic effects of Juniperus excelsa leaf extract on esophageal cancer cell line KYSE-30 and healthy fibroblast cells (HU02 cells).
Methods: KYSE-30 cells and HU02 cells were cultured in DMEM medium. The cells were treated with different concentrations (1, 10, 100, 500 μg/ml) of the J. excelsa leaf extract for 24 and 48 hours. The cytotoxic effects of the extract were assessed using the MTT assay. Data were analyzed using SPSS (version 19) and GraphPad Prism 5.
Results: According to results of the MTT assay, the Juniperus excelsa’s leaf extract exerted significant cytotoxic effects on esophagus cancer cell line (KYSE-30) and healthy fibroblast cells (HU02) in a time- and dose-dependent manner (P<0.05).
Conclusion: The J. excelsa leaf extract has cytotoxic effects against KYSE-30 esophageal cancer cells while causing lesser toxicity on healthy fibroblast cells. Our findings suggest that the potential anticancer effects of this extract should be further exploited in future studies.
Keywords: Cytotoxic, MTT, Hu02, Kyse-30, Juniperus excelsa.
Helena Hanif, Ameneh Elikaei, Hossein Vazini, Ali Mohammadi,
Volume 15, Issue 1 (Jan-Feb 2021)
Abstract
Background and objectives: The spread of infectious diseases and malignant diseases has been increasing in the recent years. The use of chemical drugs, in addition to the development of drug resistance, also cause serious side effects. We conducted the present study to examine the antibacterial, antiviral, and anti-cancer effects of E. camaldulensis as a herbal remedy.
Methods: We extracted E. camaldulensis using a hydroalcoholic solution. The antiviral effect of the plant was investigated at the time of the Herpes simplex virus entry and once the virus entered the cell. Moreover, we evaluated MIC and MBC of E. camaldulensis on Klebsiella pneumonia, Staphylococcus aureus, Streptococcus pyrogens, Streptococcus agalactiae, Acinetobacter baumannii, and Corynebacterium glutamicum. For the evaluation of cell cytotoxicity, HFF-2 (NCBI: C163) and A549 )ATCC: CCL81) cell lines were utilized.
Results: The results of the cytotoxicity test indicated that both cell lines were sensitive to the hydroalcoholic extracts of E. camaldulensis. The MIC for A. baumannii, K. pneumonia, and C. glutamicum was 6.25 µg/ml, and the MIC for S. aureus, S. pyogenes, and S. agalactiae was 12.5 µg/ml. MBC was evaluated as 25 µg/ml for S. aureus, S. pyogenes, and S. Agalactiae. It was 12.5 µg/ml for A. baumannii, K. pneumonia, and S. Agalactiae. IC50 value on entering the virus into the cell was 40 µg/ml, and following the absorption of the virus, the IC50 value was 80 µg/ml.
Conclusion: The results of this study demonstrated that E. camaldulensis is of antibacterial, antiviral, and anti-cancer potentials and could be used as a candidate for the preparation of a new drug.
