Nakisa Zarrabi Ahrabi, Hedyeh Ghadiripour, Seyed Mehdi Tabaie,
Volume 16, Issue 5 (Sep-Oct 2022)
Abstract
Background and objectives: Human Dermal Fibroblasts (HDF) are involved in the production of the extracellular matrix, formation of the connective tissue, and wound healing. Considering the role of ciprofloxacin in the treatment of skin infections and the subsequent oxidative stress as well as the protective effects of vitamin E and low power laser against inflammation and oxidative stress, we evaluated combined effects of low power laser and vitamin E on inflammation and oxidative stress in HDF cells treated with ciprofloxacin.
Methods: Morphology of the cells was studied using an inverted microscope. Viability of the cells was assessed using the MTT assay, and the concentration of reactive oxygen species was determined after exposure of the cells to ciprofloxacin (5, 25, 50, 75, and 100 μg/ml), vitamin E (1 mg/ml), and low power laser (660 nm; power density: 30 mW.cm−2).
Results: The survival rate of the cells increased significantly after the treatment with ciprofloxacin, vitamin E, and low power laser compared with the cells treated with ciprofloxacin and vitamin E (p<0.001). The amount of reactive oxygen species increased in the treated cells when compared with those only treated with ciprofloxacin and vitamin E.
Conclusion: The low power laser treatment has favorable effects on the growth of HDF cells, which can be beneficial for wound healing, even in the presence of ciprofloxacin.
Fereshteh Hematyar Tabatabaie , Ali Asghar Moshtaghie, Ali Asghar Rastegari, Hashem Nayeri,
Volume 18, Issue 2 (Mar-Apr 2024)
Abstract
Background: Certain trace elements, like cerium, have the potential to disrupt iron metabolism. This study explored the impact of cerium on intestinal iron absorption, focusing on the initial stage of iron metabolism. We employed the rat everted gut sac (EGS) segments to assess the interference caused by cerium. The primary objectives of this study were to examine the absorption of cerium in the intestines and to compare iron absorption in the presence and absence of cerium.
Methods: For the EGS experiment, segments of the rat's duodenum, ileum, or jejunum were promptly excised, cut into 5-6 cm segments, and rinsed with a physiological solution. These freshly prepared rat EGS segments were then incubated in Earle's medium containing iron (III) and/or cerium (III). We examined the impact of ascorbic acid, glucose, and different time intervals on the intestinal absorption of cerium and iron. Specifically, we investigated how glucose (5 mM) and ascorbic acid (2.8 mM) affected the absorption of cerium and iron at various concentrations (ranging from 0 to 200 mg/L). Additionally, we assessed the interfering effect of cerium on iron absorption.
Results: The results indicated that the maximum intestinal absorption of Fe (III) and Ce (III) occurred at a concentration of 200 mg/L. Furthermore, it was observed that their uptake increased following the reduction by ascorbic acid. The absorption of these elements also rose in the presence of glucose, suggesting energy-dependent transport. Additionally, a consistent cerium concentration was found to decrease iron absorption by 24.3% (P ≤ 0.05).
Conclusion: Based on the results, cerium likely reduces iron uptake by competing with iron. Cerium can also disrupt iron metabolism and lead to iron-related metabolic disorders. However, further studies at the molecular and intracellular levels are needed to gain a better understanding of this mechanism.
