Showing 2 results for Pangh
Mahsa Shad, Ayeneh Hagieh Pangh, Farideh Tohidi,
Volume 18, Issue 3 (May-Jun 2024)
Abstract
Background: Soil contamination with parasites poses a significant and concerning risk for the transmission of parasitic infections to humans and animals. Considering the crucial role of soil as a potential source of parasite transmission, this study aimed to investigate the presence of helminth and protozoan parasites in the soil of public parks in Gorgan City.
Methods: In this study, a total of 80 soil samples were collected from 16 public parks in Gorgan City in 2022. The soil samples were collected from various areas within the parks, including flower-making, children's play areas, walking paths, sitting areas, and garbage dumps. To isolate the parasites from the soil samples, a saturated solution of sodium nitrate was utilized. The samples were carefully examined under a microscope, and all isolates were stained and subjected to further investigation using special acid-fast stain.
Results: Toxocara eggs were the most commonly detected parasite, present in 40% of the samples (n=32). Coccidia oocysts were found in 12.5% of the samples (n=10), which could potentially be Sarcocystis or Toxoplasma oocysts. Mites were also detected in 12.5% of the samples (n=10). Additionally, Cryptosporidium oocysts were identified using Ziehl-Neelsen's modified staining method, and they were found in 10% of the samples (n=8).
Conclusion: Health education plays a vital role in raising awareness about preventive measures. Collecting reservoirs of parasites (Such as dogs and cats) and planning for the proper disposal of human waste also play an important role in prevention of this disease.
Dr. Fatemeh Livani, Dr. Faramarz Koohsar, Dr. Farideh Tohidi, Dr. Mitra Sharbatkhori, Dr. Roghiyeh Faridnia, Dr. Ayeneh Hajieh Pangh, Dr. Mehdi Khoshrou, Dr. Hamed Kalani,
Volume 19, Issue 1 (4-2025)
Abstract
Background and objectives: Cutaneous leishmaniasis (CL) is a prevalent infectious zoonotic disease between human and animals. Golestan province is one of the important centers of CL in Iran. Current method for detecting Leishmania parasite in patients is Giemsa-stained direct smear from skin ulcers; however, PCR is a strongly recommended method for epidemiological studies. The aim of this study is to investigate Leishmania species in negative ulcer smears from patients suspected of having CL referred to Aq Qala health center using multiplex nested PCR method.
Methods: This study was performed on 72 negative ulcer smears from patients suspected of having CL referred to Aq Qala health center, Golestan province, northeastern Iran, during August 2019 to April 2020 using multiplex nested PCR method to detect Leishmania major and Leishmania tropica species.
Results: Out of 72 samples, 4 (5.55%) samples were positive by multiplex nested PCR. Moreover, all positive samples are related to Leishmania major species.
Conclusion: Detecting of Leishmania species is strongly recommended in negative ulcer smears from patients suspected of having CL using multiplex nested PCR method.
