Abstract Background & Objectives: Thalassemia syndromes isone of the inherited disorders in which one or more globulin chains are affected. On the basis of clinical symptoms, thalassemias are categorized as minor, intermediate, and major. Minor beta -thalassemia is a mild microcytic hypo chromic anemia in most cases asymptomatic and HbA2 is more than normal. Materials & Methods: This study carried out on 813 blood samples obtained from male high school students of Aliabad Katool, in the north of Iran. After detecting red blood cell indices of the subjects whose MCV was less than 80fl HbA study performed. Results: The MCV of 8.24% of the subjects was less than 80fl.34 of 67 had HbA2 of less than 3.5 %(normal) while the rest more than 3.5%(minor). Conclusions: Since about 50% of microcytic anemic patients were the carrier of beta-thalassemia and it is highly Prevalent in this region (4.06%), we recommend to investigate HbA2 in people with MCV less than 80 fl. Keyword: Thalassemia, Thalassemia minor, HbA2, Iran

Abstract Background and objectives: Vibrio Cholera is one of the causative agents that can easily lead to death if the appropriate therapeutic measures are not taken in time. The purpose of this study is to identify the antibiotic sensitivity of the isolated V. choleras from the patients during the outbreak of Cholera in Golestan province, 2005. Materials and Methods: The Subjects were 97 positive cultured samples sent by different laboratories of Golestan province health centers .First, these samples were confirmed with microbiological and biochemical tests. Then, their antibiotic sensitivity was examined by Kirby –Bauer method (12 routine drugs were used).To obtain better results, the tests carried out in parallel by two expert technologists. Results: The participants, aged 29.8 +- 16 years, are male (52.6%) and female (47.1%). The highest antibiotic susceptibility pattern is related to Cephotaxime (91.6%), Ceftizoxime (87.8%), Oxcy Tetracycline (76.1%) while the most antibiotic resistance is referred to Nalidixic Acid (84.5%), Fourazolidin (50.7%) and Erythromycin (50.7%). During outbreak, Antibiotic resistance was not increased. Both technologists reports regarding Ampicillin, Tetracycline, Nalidixic Acid (kappa of 94%, 89%, 82%, p value<0.05) have the most agreement while it is not true about Erythromycin and Fourazolidin disks. (Kappa 67% and 62%, p value<0.05) Conclusion: Because of the resistance of Vibrio cholera to different antibiotic agent, it is important to determine their pattern to control and prevent the spread of drug resistance species. Key words: Vibrio cholera, Inaba, antibiotic resistance, Golestan