Showing 2 results for Nasirzadeh
Seyed Zanyar Athari, Mir Alireza Nourazar, Mohammadreza Nasirzadeh,
Volume 16, Issue 3 (May-Jun 2022)
Abstract
Background and objectives: Increased oxidative stress and altered antioxidant levels play an essential role in the pathogenesis of diabetes mellitus. Menopause is associated with increased food intake, weight gain, metabolic disorders, and increased level of inflammatory and oxidative stress factors. This study evaluated effects of oleuropein extract on brain tissue and serum oxidative status in ovariectomized diabetic rats.
Methods: In this study, 40 female Wistar rats weighing 250±20 grams were randomly divided into four groups: control, sham (surgery without ovariectomy), ovariectomy+diabetes, and ovariectomy+diabetes+oleuropein supplementation. Diabetes was induced by intraperitoneal injection of streptozotocin. The rats in the treatment group received 60 mg/kg of oleuropein for 30 days by oral gavage. Finally, blood glucose, superoxide dismutase, glutathione peroxidase, total antioxidant capacity, and malonaldehyde levels were evaluated in serum and brain tissue samples.
Results: Weight, blood glucose level, and antioxidant enzymes activity increased significantly in the ovariectomy+diabetes group compared to the control group.
Conclusion: This study suggested that the administration of oleuropein extract has beneficial effects on blood glucose level and antioxidant status in serum and brain tissue of ovariectomized diabetic rats.
Zahra Nasirzadeh, Seyedeh Tooba Shafighi,
Volume 20, Issue 1 (1-2026)
Abstract
Abstract
Background and Objective: Currently, healthcare-associated infections (HAIs) are rising alarmingly due to antibiotic-resistant gram-negative bacteria, particularly Pseudomonas aeruginosa and Acinetobacter species. The study in Rasht focuses on identifying phenotypic and molecular metallo-β-lactamases (MBLs) in clinical isolates of Pseudomonas aeruginosa and Acinetobacter that display resistance to Imipenem.
Methods: In this study, 52 Acinetobacter and 25 Pseudomonas aeruginosa samples were collected from medical centers in Rasht. The clinical isolates were analyzed using various biochemical tests. The antibiotic resistance patterns of the isolates were determined by the disk diffusion method, following CLSI guidelines. Isolates producing metallo-β-lactamases (MBLs) were identified using the IPM+EDTA combined test method. Genomic DNA was subsequently extracted from the isolates using a commercial kit, and the presence of blaIMP and blaVIM genes was determined by PCR.
Results: According to the findings of this study, the highest percentage of resistance in Acinetobacter was observed with cefotaxime antibiotics at 94.23%, and in Pseudomonas aeruginosa with ceftazidime at 84%. Among the clinical isolates not susceptible to Imipenem, 18 (40%) were Acinetobacter and 2 (15.38%) were Pseudomonas aeruginosa, as determined by the combined disc method. The blaIMP and blaVIM genes were detected in 7 (13.46%) and 11 (21.15%) of the Acinetobacter samples, respectively, and in 8 (32%) and 5 (20%) of the Pseudomonas aeruginosa samples, respectively.
Conclusion: These results suggest that the blaIMP and blaVIM genes play important roles in the antibiotic resistance and potentially influence the virulence of Acinetobacter species and Pseudomonas aeruginosa in the region
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