---

Spring summer 2010, Vol.4, No.1 /74 Medical Laboratory Journal Severity of Oxidative DNA Damage in Gastric Tissue of Smoker and Non-smoker Patients with Dyspepsia Abstract Background and Objectives: Cigarette smoking is associated with an increase in risk of peptic ulcer and Gastro-Intestinal cancer. Toxic materials in smoke and tar have a significant role in production of carcinogenic complexes, injury to DNA and cellular proliferation in gastric cancer. The study was designed to compare the rate of injury to DNA in gastric tissue of smoker and non-smoker patients with active peptic ulcer. Material and Methods: In this Case-Control study, the case group composed of 43 smoker patients aged 45.30±13.16 with active peptic ulcer (14 female & 29 male) referred to gastroenterology clinic. The first control group consisted of 43 non-smokers without peptic ulcer (13 female & 30 male) with mean age of 42.67±16.04, and the second control group included 43 smokers without peptic ulcer (16 female & 27 male) with mean age of 44.58±12.07, and the third ones had 43 non-smoker patients with active peptic ulcer (20 female & 23 male) with mean age of 45.37±13.39. The rate of gastric mucosa DNA damage in the four groups was measured by calorimetrically method. Results: The DNA damage in gastric mucosa of smoker patients with active peptic ulcer(28.05±5.54 AP/100000bp) is higher than those of the three control groups (p<0.0001 in all case). Conclusion: Results of this study approve the direct relation between increase in DNA damage and toxic complexes existing in smoke and tar of cigarette. Key Words:Cigarette Smoking, DNA Damage, Active Peptic Ulcer Dolatkhah H Research Center of Gastroenterology and Hepatology, Tabriz University of Medical Sciences Somi MH Research Center of Gastroenterology and Hepatology, Tabriz University of Medical Sciences Fattahi E Research Center of Gastroenterology and Hepatology, Tabriz University of Medical Sciences Estakhri R Research Center of Gastroenterology and Hepatology, Tabriz University of Medical Sciences Dolatkhah N Talegani Hospital, Tabriz University of Medical Sciences Mirza-Aghazadeh A Dept. of Basic Sciences, Paramedical Faculty , Tabriz University of Medical Sciences Nourazarian,M Clinical Laboratory of Emam Reza Hospital, Tabriz University of Medical Sciences Pourasghari B Clinical Laboratory of Emam Reza Hospital,Tabriz University of Medical Sciences Corresponding: Dolatkhah, H E-mail: dolatkhahh@gmail.com

---

Abstract Background and Objective: Recently, diabetes mellitus has been known as one of the main cause of upper gastrointestinal symptoms. Since a high prevalence of H. Pylori in diabetic patients has been reported, we aimed to evaluate the level of gastric juice Nitric Oxide (NO°), Oxidative Stress and Glycated Hemoglobin. Material and Methods: In case group, the participants were 60 diabetic patients infected with H. Pylori, and in control groups 60 diabetic patients without H. Pylori and 60 healthy individuals. The level of NO° in gastric juice was measured calorimetrically and the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in gastric biopsy was determined using standard methods. The percentage of Glycated Hemoglobin (HbA1C) was measured by ion exchange chromatography. Results: In case group compared to controls, significantly increased level of blood HbA1C, nitric oxide in gastric juice, activity of SOD and GPX in the gastric mucosa were observed (p<0.0001). Conclusion: A significant increase of glycated hemoglobin in diabetic patients with H. Pylori and high activity of antioxidant enzymes in the case group may indicate a high production of reactive oxygen species and the presence of oxidative stress in these patients. Key Words: Diabetes Mellitus, H. Pylori Infection, Glycated Hb, Nitric Oxide, Oxidative Stress