Showing 3 results for Javid, N
M Talkhabifard, M, N Javid, N, A Moradi, A, A Ghaemi, A, A Tabarraei, A,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Human Cytomegalovirus (CMV) is an important cause of congenital viral infection that can lead to serious diseases and complications in infants. Application of rapid, sensitive, and specific HCMV detection methods is necessary for congenital infection detection. We aimed to optimize the use of PCR and ELISA for detection of HCMV in infants. Material and Methods: PCR–ELISA was performed by using specific primers and probe for detection of the HCMV glycoprotein B gene. First, the extracted DNA from urine samples and controls were labeled by digoxigenin during DIG-labeling PCR. After that, Biotin-labeled probe captured the DIG-labeled PCR products. The probe-PCR product hybrid is immobilized on a streptavidin-coated Microtiter plate, and detection was confirmed by proxidase-conjugated anti-digoxigenin antibody, and calorimetric substrate. Results: The clinical Human CMV strains isolated from16 patients were detected by this method. The optimized PCR-ELISA method was able to detect less than100 copies of HCMV genome. There was no non-specific reaction. Conclusion: PCR-ELISA can be applied as a sensitive, specific and reliable method for Semi-quantitative CMV detection in clinical samples. Keywords: Cytomegalovirus, Glycoprotein B, PCR-ELISA, Semi-Quantitative
Gol Mohammadi, R, Tabaraei, A, Abbasi, A, Khademi, N, Mahdavian, B, Javid, N, Kaleji, H, Kamasi,a, Bazoori, M, Moradi, A,
Volume 9, Issue 1 (March, April[PERSIAN] 2015)
Abstract
Abstract
Background and Objective: Highly Active Antiretroviral Therapy (HAART) can effectively prevent the progression of HIV-1 replication and increase life expectancy. There are numerous causes of treatment failure and the leading one is drug resistance. Thus, we aimed to determine the HIV RT gene drug resistance mutations in patients treated with antiretroviral medications.
Material and Methods: In this cross - sectional study, venous blood was taken from 130 HIV-positive patients treated with antiretroviral medications. In order to determine drug resistance mutations, RT-PCR and PCR steps were performed using RT gene specific primers. Subtypes and mutations in the virus genome were determined using the Stanford HIV drug resistance sequence database.
Results: In 122 treating patients, most of the major mutations were associated with nucleoside and non-nucleoside drugs. subtype A in 66.4%, subtype D in 26.2% and subtype B in 7.4% of the participants were reported. They were resistant to Nucleoside RT Inhibitor drugs (23.7%) and Non-Nucleoside RT Inhibitor drugs(30.3%). The highest were related to Nevirapine (21.3%) and Efavirenz (19.7%) and the lowest to both Tenofovir and Zidovudine (91.5%).
Conclusion: The use of two nucleoside RT inhibitor drugs combined with one protease inhibitor drug could be effective in the treatment of HAART.
Key words: HIV, Nucleoside RT Inhibitor, Non- Nucleoside RT Inhibitor
Koochaki, Gh M, Talebi, R, Bazouri, M, Javid, N,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract
Background and Objective: Septi scrub is a new product based on chlorhexidine 4%, which is produced for surgical scrub in Iran. This study aimed to compare the disinfecting effect of Povidone Iodine and Septiscrub in surgical scrub.
Material and Methods: this crossover experimental study, one-week interval, was conducted to compare Povidone Iodine and Septiscrub. Three samples for microbial cultures were obtained from each participant before, shortly and 40 minutes after scrubbing.
Results: The findings showed that the effect of Povidone iodine and Septiscrub solutions was significant on microbial growth of measure (P< 0.05). In both solutions, there was a significant difference in the rate of microbial growth between before and after scrub stages (P< 0.05). But between immediately after scrub and 40 minutes after was not significant (P< 0.05).
Conclusion: given the equal effect of two solutions and lesser side effect of Septiscub , it is recommended that Septiscrub be used instead of Povidone iodine 7.5%.
Keywords: Surgical Scrub; Povidone Iodine; Septi Scrub.
