Showing 64 results for Jan
A Marjani, A.r. Mansoorian, H. R. Joshaghani, K Heydari, A Sarikhani,,
Volume 1, Issue 1 (Spring - Summer 2007 [PERSIAN] 2007)
Abstract
Abstract Background and objective: Exposure of red blood cells to oxygen radicals can induce Lipid proxidation, hemoglobin damage and hemolysis of erythrocyte .The present study was designed to determine the alteration of plasma lipid peroxidation and erythrocyte Superoxide Dismutase and Glutathione Peroxidase enzyme activities in stored blood and to find out the quantitative alterations and the useful length of stored blood. Materials and Methods: First, the whole blood form 10 donors was taken. Then Red Blood Cells(RBC) were counted, the levels of Potassium(P) and lactate dehydrogenate activity(LDH) were measured to determine the amount of hemolysis, the plasma levels of malondialdehyde (MDA), erythrocyte Superoxide Dismutase(SOD) and Glutathione Peroxidase(GPx) were studied for determination of lipid peroxidation and antioxidant enzyme activities at the days of 0,1,3,5,7,9,11,13,15,17,19,21,23,25,27,29,31,33 and 35 of the storage. Results: upon storage time, the plasma levels of malondialdehyde (MDA) and Potassium and lactate dehydrogenate activity increased (P< 0.05) whereas erythrocyte Superoxide Dismutase and Glutathione Peroxidase enzyme activities and Red Blood Cells decreased (P< 0.05). The alterations of MDA, SOD, GPx, P, LDH and RBC in the measurement days were as follows: MDA, P and LDH significantly increased at the day of 9, 5 and 5 whereas SOD, GPx and RBC decreased at the day of 11, 7 and 29 respectively. Conclusion: The results of this study showed that the increased level of MDA and decreased SOD and GPx in stored blood can cause the beginning of hemolysis of erythrocyte therefore, it is necessary to control these factors before storing the donated blood. Keywords: lipid peroxidation, Superoxide Dismutase, Glutathione Peroxidase
Hr Joshaghani, Aa Shirafcan, Aj Marjani,
Volume 1, Issue 2 (Autumn – Winter 2008[PERSIAN] 2007)
Abstract
Abstract
Introduction:
methionine. Many reports confirm the correlation between hyper
homocysteinemia and cardiovascular disease. This study was aimed
at determining the effect of B12 and folate deficiency on the
homocysteine level after myocardial infarction.
Homocysteine is produced by demethylation of
Materials and methods:
study were patients with myocardial infarction (N = 48) and healthy
patients (N = 48) Eliza method was used to assay Homocysteine and
RIA for folic acid and vitamin B12.
The subjects of This descriptive-analytic
Results:
(30.3 ± 5.3 μm/l) and the control group (11.1 ± 3.1) is significant (p<
0.001). There is no significant difference between Serum B12 in case
(297.1 ± 208.9 pm/l) and control group (261.5 ± 205.3) and it is true
about Serum folic acid of case (3.9 ± 2.9 ng/m) and control group
(4.3 ± 3.5). The homocysteine level of all patients and four of
healthy subjects is higher than normal. The folic acid Level of 11
patients and four healthy subjects is less than normal.
the difference between the homocysteine Level of the case
Conclusion:
of control group and this difference is not related to decrease of B12
Level, Physicians must pay attention to The other risk factors.
since the homocysteine level of patients is there times
Key words:
cobalamine, cardiovascular disease.
Folic acid, Homocysteine, Myocardial Infarction,
Biranvand E, Abedian Kenary S, Ghaheri A, Rezaee M S, Hasannia H, Nasrolahi M, Parsaee Mr, Ahanjan M, Biranvand B, Ahmadi Basiri E,
Volume 5, Issue 1 (spring-summer[PERSIAN] 2011)
Abstract
Abstract Background and objectives: Interferon-Gamma and interferon Gamma receptor (IFNγ ⁄ IFNγR1) are the main genes associated with susceptibility to tuberculosis. We aimed at studying on interferon-Gamma Gene polymorphism(- 56 C/T) in people suffered from tuberculosis (TB). Material and Methods: In this case-control study, the subjects were 62 individuals with TB and 74 healthy ones. Genomic DNA was extracted by DNA isolation kit(Roche Corporation), and genotype identification was performed by polymerase chain reaction-Restriction fragment length polymorphism (PCR-RFLP). Chi square and logistic regression, using SPSS soft ware (version 18), was used to compare genotype and alleles between case and control groups. Results: The frequency of TT genotype in tuberculosis patients and healthy person are 43.5% and 17.5%, respectively. Based on Logistic regression (odd ration 0.148, p=0.0006), there is significant difference between Case and Control. In addition, the frequency of T allele is, in case group, 62.09 % the difference between case and control is significant, based on Logistic regression (odd ratio: 0.418, P=0.028). Conclusion: It is implied that -56C/T is associated with IFNγR1 promoter in tuberculosis patient. It is found to be associated with increased susceptibility to tuberculosis. Key words: Tuberculosis, IFNγR, PCR-RFLP
Khoshdel Rad N, Mashayekhi F, Mirzajani E,
Volume 6, Issue 1 (spring-summer[PERSIAN] 2012)
Abstract
Abstract Background and objectives: C-Met is a proto-oncogene that encodes a protein known as hepatocyte growth factor receptor (HGFR). The HGF receptor possesses tyrosine -kinase activity and it is essential for embryonic development, wound healing and cancer. Many proteins are proteolytically released from the surface by a process known as ectodomain shedding. Shedding occurs under normal physiologic conditions and can be increased in certain pathologies. C-Met can be seen among many receptors for which ectodomain shedding has been shown. The aim of this study was to determine the concentration of soluble c-Met in the cerebrospinal fluid (CSF) and serum samples of patients with viral and bacterial meningitis. Material and Methods: in this study, 75 CSF and serum samples of patients with bacterial meningitis, 71 with viral meningitis and 82 normal controls were investigated. The soluble c-Met concentration was determined by enzyme linked immunosorbent assay (ELISA). Result: the amount of soluble c-met in CSF of patients with bacterial meningitis ( 83.91±5.50), viral meningitis ( 80.41±4.71) and control group ( 22.66±3.39) are compared with that in serum of patients with bacterial meningitis ( 561.58±25.87), viral meningitis ( 550.50 ±34.34) and control group ( 256.25±18.55). There is significant increase in the CSF and serum’s soluble c-Met expression in the patients with meningitis, in comparison with control group. Conclusion: The data presented here indicate that soluble c-Met is a constant component of human serum and CSF, but it can not be used for differentiating bacterial meningitis from viral meningitis. Key words: Soluble c-Met, concentration, cerebrospinal fluid, serum, meningitis
H Mahmoudjanlou, K, A Moradi, F Shakeri, M Babaii Koochaksarii, N Mansoor Samae,
Volume 6, Issue 2 (Autumn- Winter [PERSIAN] 2012)
Abstract
Abstract
Background and objectives: the increasing use of antibiotics, especially the third generation cephalosporins, is an important factor in the spread of antibiotic resistance in bacteria. The main reason for the development of resistance phenotype such as Extended Spectrum Beta Lactamas (ESBL) is the extensive use of broad-spectrum cephalosporins. In phenotypic survey, the Phenotyping confirmatory test and the minimum inhibitory concentration (MIC) are used. In this study, the prevalence of the isolates resistant to third generation cephalosporin (cefotaxime) was determined based on MIC.
Material and Methods: form September 2010 to September 2011, 75 isolates of Klebsiella pneumoniae were collected from the infections of inpatients and outpatients, referred to state and private laboratories of Gorgan. For all of the Klebsiella pneumoniae strains, MIC determination using E-test (company Liofilcheme-Italy) was performed.
Results: According to the MIC results, 26 samples (34.6%) are resistant to cefotaxime 22 isolates are completely resistant to concentration of 256μg.
Conclusion: Because of the importance of risk of becoming ESBL, further studies are needed to clarify the ESBL in the region.
Keywords: ESBL, MIC, Klebsiella pneumoniae, Cephalosporin
F Safarnezhad Tameshkel, Mr Khatami Nejad, A Nasrollahi, P Rahdari, F Gholam Hossein Poor, S Kazemi Afarmejani,, A Rahnavard,
Volume 6, Issue 2 (Autumn- Winter [PERSIAN] 2012)
Abstract
Abstract Background and objectives: Giardiasis is a parasitic infection of small intestine, with a worldwide distribution and the prevalence of Giardia in different parts of the world varies between 1 to 25%. Plants have the vast range of antimicrobial and antifungal activity that can be identified as alternative treatments for bacterial and parasitic pathogens, the same as Giardia. In this study, the methanol extracts of eucalyptus plants, Satureia hortensis and Heracleum glabrescens, on Giardia cysts were studied in vitro. Material and Methods: The cysts were isolated from the feces using a modified Bingham. After counting by Hemusytumetr, they were placed near by 200 mg / ml, 100 mg / ml and 10 mg / ml of the extracts prepared by DMSO for 30 and 60 minutes. Then, the number of dead and live cysts was counted under a microscope. Results: the fatality effect of the extracts in 60 minutes is higher than those of 30 minutes. The methanol extracts of Satureia hortensis, Eucalyptus and Heracleum glabrescens with the dilution of 200 mg/ml in 60 mins have the fatality effect of 84/3%, 63/3% and 44%, respectively. The highest fatality(84.3%) on Giardia cysts is related to Satureia hortensis with the dilution of 200 mg/ml in 60 mins and the Lowest(27%) is related to Heracleum glabrescens with the dilution of 10 mg/ml in 30-minute period. The significant relationship between the plant type and the fatality of methanol extracts is observed. Conclusion: the methanol extracts of Eucalyptus, Heracleum glabrescens and especially Satureia hortensis have anti-parasitic effects in the laboratory conditions. Thus, they can be used in the future, instead of the chemical antiparasitic drugs. Key words: Antibacterial Giardia lamblia cysts, Eucalyptus, Satureia hortensis, Heracleum glabrescens, Tonekabon
S Royani, S Alijanpor, Z Shirbaghaei, R Khorasaninejad, Gh Roshandel, Aa Ayatollahi, Hr Joshaghani,
Volume 7, Issue 3 (Autumn 2013)
Abstract
Abstract
Background and Objective: Of the most common hypochromic microcytic anemia are iron deficiency anemia and minor thalassemia, which are common in Iran and their differential diagnosis is extremely important. The level of 25-hydroxy vitamin D is the indication of vitamin D blood status. The aim of this study was to compare serum levels of vitamin D in people with minor thalassemia and iron deficiency anemia with healthy subjects in order to investigate the relationship between vitamin D deficiency and iron absorption.
Material and Methods: In this case-control study, 24 patients with minor thalassemia, 20 patients with iron deficiency anemia and 24 healthy individuals participated. Groups were matched for age and sex. Testing of Vitamin D level by ELISA, ferritin by quantitative luminescence method and HbA2 by column chromatography was carried out.
Results: The number of individuals with low level of vitamin D in iron deficiency group is 15 (75%), in minor thalassemia group is 8 (33/3%) and in the control group 11 (45.8%).
Conclusion: In this study, the highest percentage of vitamin D deficiency is observed in cases with iron deficiency anemia. Because of association between vitamin D and anemia, iron and vitamin D supplementation is recommended to enrich the diet.
Keywords: Anemia Minor Thalassemia Iron Deficiency Anemia Vitamin D
P Torabi, M Azimirad, Z Hasani, M Janmaleki, H Peirovi, M Alebouyeh, Mr Zali,
Volume 8, Issue 1 (spring[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: This study was aimed to determine the extent of bacterial contamination and drug resistance patterns of isolates colonized in colonoscope and endoscope and in relevant personnel.
Material and Methods: A total of 107 samples were obtained from staff of endoscopy and colonoscopy units (SEU and SCU) and gastroenterological imaging equipment. For isolation and identification of the bacteria, swab culture method and biochemical identification test were used, respectively. Antimicrobial resistance profiles, multi-drug resistance (MDR) patterns and phenetic relatedness of these isolates were also analyzed according to standard methods.
Results: Most frequent pathogenic bacteria among the SEU and gastroenterological imaging related equipments were included S. aureus (20.8 % and 0 %) Enterococcus spp. (0 % and 5.4%) Pseudomonas spp. (0% and 13.5 %), and Clostridium difficile (0% and 12.5%). Analysis of resistance phenotypes showed a high frequency of MDR phenotypes among the SEU (82.1%), and also in endoscopes, colonoscopes, and other equipments (20%, 50% and 100%, respectively). Phylotyping of S. epidermidis isolates showed the role of staff in transmission of resistance strains to medical equipments and also circulation of strains with identical resistance phenotype among the studied samples.
Conclusion: High frequency of pathogenic bacteria in colonoscopes, endoscopes and in the staff of endoscopy & colonoscopy units, and also contamination of these instruments with MDR pathogens emphasize the need for proper disinfection of endoscopes and colonoscopes and also instruction of staff in these units.
Key words: Bacterial Contamination Endoscope Colonoscope Antimicrobial Resistance Gastrointestinal Disease.
Ar Niazi, F Koohsar, F Ghaffarifar, H Ziaei-Hezarjaribi,, F Mesgarian, on Jorjani,
Volume 8, Issue 2 (summer 2014[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: Culture, microscopic method is a gold standard method for identification of Lishmania parasite. The use of Molecular methods such as RT- PCR compared to microscopic methods has a higher sensitivity and specificity however, it is not widely used due to its expensive equipment and the time requested. The use of nucleic acid sequence based amplification (NASBA) method is highly valuable for diagnosis of live parasite because there is no need for to use Thermo cycler. We aimed to assess sensitivity and specificity of NASBA for molecular detection of cutaneous Leishmaniasis.
Material and Methods: First, the RNA was extracted from 28 skin biopsies suspected cutaneous Leishmaniasis. Then, by means of specific primers designed for 18srRNA region, this region was amplified using NASBA isothemal amplification. To increase the sensitivity, the product was electroforesed in TBE (IX) buffer, using Syber Gold Flourecent probes. Using specific primers, RT- PCR was conducted on the samples too.
Result: For diagnosis of Leishmania parasites, NASBA and RT-PCR had the sensitivity of 81% and 51%, respectively, and specificity of 100%.
Conclusion: NASBA isothermal method with high sensitivity and specificity can be applied for identification of cutaneous leishmaniasis.
Keywords: Cutaneous Leishmanisis, NASBA, 18S rRNA
Mahmoudjanlou, H, Ghazisaeedi, K, Shakeri, F, Ghaemi, Ea,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Klebsiella pneumoniae is one of the agents causing nosocomial infection therefore, we decided to report the prevalence of Klebsiella pneumoniae caused infection. Material and methods: The frequency of Klebsiella in culture media samples of Panje Azar hospital was studied in 2011-2012. After determination of the species with biochemical methods and determination of resistance to third generation cephalosporins, the existence of responsible genes for this resistance was investigated using specific primers. The PCR product for CTX-M gene was sequenced. Results: During the study, 70 isolates of Klebsiella were isolated in that 51 (72.8%) related to three months of November, December and January. Except for the one related to November, other ESBL cases belonged to these three months. Based on molecular investigation of ESBL genes, these isolates at least were in 3 types and had a high frequency in Internal, female and Emergency wards. Conclusion: The present report implied a sudden prevalence of Klebsiella pneumoniae that detected and controlled by a correct monitoring. Keyword: Klebsiella Pneumoniae, ESBL, CTX-M
Mohammad Taghvaie, N, Jalali, Mt, Ghasemi Falavarjani, M, Shahbazian, Bb, Saki, A,
Volume 9, Issue 2 (may,jun 2015[PERSIAN] 2015)
Abstract
Background and Objective: According to recent changes in diagnostic criteria for diabetes, the harmonization of results obtained from various methods and systems by considering their accuracy and precision is essential. This study aimed to evaluate the accuracy, precision and consensus of some routine laboratory glucose kits in comparison with Hexokinase reference method.
Material and Methods: The participants were 38 diabetic patients with fasting blood sugar (FBS) ≥126 mg/dl, nine prediabetic patients with FBS of 100-125 mg/dl, 15 non-diabetic people with FBS of 60-100 mg/dl and 9 hypoglycemic patients with FBS of ≤60 mg/dl. Their FBS were measured by four routine laboratory glucose kits: Glucose oxidase on BT3000 analyzer with an open system and Hexokinase reference method on a close system (COBAS INTEGRA®400plus analyzer, Roche kit). Accuracy and precision were determined and compared with reference method.
Results: Glucose oxidase methods showed a good agreement with the reference method, in Correlation Coefficient>0.99. based on regression analysis, the slope of 1.114 for Pars Azmoon, 1.105 for Bionik, 1.121 for Elitech and 1.087 for Human were reported (P<0.05). Error of the mean for ParsAzmoon was 12.79, for Bionik 10.86, for Elitech 12.58 and for Human were 8.46. Coefficient of Variation showed more imprecision for Bionik and Human kits.
Conclusion: given the same almost standard errors, standard devisions and regression analysis, the precision in four methods is the same but in comparison with Hexokinase, reference method has not the accuracy.
Keywords: Blood Glucose, Glucose Oxidase, Hexokinase, Methods, Consensus
Marjan Vahedi, Parvin Farzanegi,
Volume 9, Issue 4 (sep,Oct 2015 2015)
Abstract
Abstract
Background and Objectives: Diabetes induced oxidative stress plays an important role in pathological damage to the heart and liver by increased production of extracellular matrix. It is thought that the use of medicinal plants, particularly Portulaca oleracea. L and regular exercise are effective. The aim of this study was to evaluate the effects of Portulaca oleracea. L consumption along with resistance training on cathepsin S, cystatin C and C-reactive protein (CRP) levels on type 2 diabetes patients.
Methods: In this semi-experimental study, 28 female type 2 diabetes patients with a mean age of 52 were randomly devided into 4 groups of control, exercise, supplement and supplement-exercise. Portulaca oleracea. L supplement was cosumed 7.5 g per day. Resistance training program was performed with a rubber band for 8 weeks, 3 days a week for 60 minutes with40-50% intensity, up to a maximum repetition. Blood samples were taken before and 48 hours after the last intervention.
Results: After eight weeks, cathepsin S, cystatin C and CRP levels in the supplementation and supplementaion-exercise group were significantly reduced (P<0.05). There were also significant differences between the groups.
Conclusion: Portulaca oleracea. L consumption and resistance training have each separate positive impacts on the cathepsin S, cystatin C and CRP levels, but the simultanous effect of Portulaca oleracea. L seed consumption and physical activity can lead to a better efficiency.
Keywords: Portulaca oleracea, resistance training, cathepsin S, cystatin C, C-reactive protein, type 2 diabetes mellitus.
Hasan Kaleji, Alijan Tabaraei, Abdollah Abbasi, Naemeh Javeed , Masoud Bazoori , Reza Golmohamadi , Abdolvahab Moradi,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract
Abstract
Background and Objective: Various cellular factors affect the process of HIV activity. One of these cellular factors are structures known as microRN that are expected to be involved in controlling HIV replication and infectivity. The expression of one or a set of them may represent the patient's clinical conditions. In this study, the expression of miR-29a and miR-29b involved in regulating viral genes’ expression was evaluated in three HIV-positive groups and a healthy control group. Later, the expression level of these microRNAs was compared between the cases and controls.
Methods: Total RNA extraction was performed on the collected samples using RNx-plus kit and then the microRNA expression levels were evaluated using Relative Real-time PCR. The obtained data was entered into SPSS 22 and Graphpad softwares and analyzed using Kruskal-Wallis and Man-Whitney tests. P-value of less than 0.05 was considered as statistical significance level.
Results: The expression level of miR-29a was reduced in patients under treatment and drug-resistant patients ( P ≤ 0.05) . All three HIV-positive groups including people without drug treatment, patients under treatment and drug-resistant patients showed reduced miR-29b expression level compared to control group (P ≤ 0.05).
Conclusion: the decreased expression of miR-29a and miR-29b in patients under treatment and drug-resistant patients indicates an increased viral replication and reduced CD4 cell count. It may be possible to predict the progression of the disease by miRNA measurement or control viral replication using these mir-RNAs that requires further studies.
Keywords: HIV, expression, mir-29a, mir-29b.
Hamid Reza Joshaghani , Saeid Parvizi , Khodaberdi Kalavi , Naser Behnampour, Hadi Joshaghani , Nader Hashemi, Sahar Alijanpour,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract
Abstract
Background and Objective: Normal hemoglobin (Hb) is formed of a heme group and a protein group known as globin. Globin is made of four polypeptide chains and in hemoglobinopathies, the structure of one of these four polypeptide chain becomes abnormal. Cellulose acetate method is a common way to differentiate haemoglobinopathies. Inability to identify the components of Hb low concentrations and incapability to isolate all Hb types are among the disadvantages of this method. The aim of this study was to report the prevalence of hemoglobinopathies in the North of Iran by capillary electrophoresis method.
Methods: All patients with suspected hemoglobinopathies, referred by physicians for electrophoresis, have been studied in a private center in the city of Gorgan, Iran. The level of HbA2, HbA, HbF and other Hb was recorded.
Results: Overall, 725 blood samples were analyzed using the capillary method. HbE was reported in 2 patients, HbH was observed in 2 patients and Hb Barts was reported in 3 patients. Using the capillary method, among patients with the SDG area, only 4 of 38 (10.52%) had HbS and the majority of them (89.48%) had HbD.
Conclusion: HbD is the most common hemoglobinopathy in the North of Iran.
Keywords: Hemoglobinopathy; hemoglobin D; Capillary Electrophoresis; Iran
Farzane Salarneia , Sare Zhand , Behnaz Khodabakhshi , Alijan Tabarraei , Mohammad Ali Vakili , Naeme Javid , Masoud Bazori , Abdolvahab Moradi ,
Volume 10, Issue 1 (Jan,Feb 2016 2016)
Abstract
Abstract
Background and objective: Hepatitis B virus (HBV) is a DNA virus with high tendency toward hepatic tissue. There are currently about 3 million HBV-infected people and 350 to 400 million chronic carriers of this virus in the world. X protein plays a role in the over-expression of oncogenes, carcinogenicity of liver cells and overlaps with the basal core promoter of the virus. Mutations at specific nucleotides of this region increase viral replication and liver disease progression. The aim of this study was to investigate the frequency of mutations at nucleotides 1762, 1764 and 1766 of HBV X gene in patients with chronic hepatitis B and hepatitis B-related cirrhosis.
Methods: In this study, 102 patients including 68 chronic hepatitis patients and 34 patients with hepatitis B-related cirrhosis were enrolled. After DNA extraction, HBV X gene was amplified and sequenced using Semi Nested-PCR. Obtained gene sequences were compared with the standard sequence of HBV virus X gene available in the gene bank (Okamoto AB033559). Then, the mutations in the gene X of HBV were identified.
Results: Comparison of the standard sequence with sequences obtained from patients showed the presence of A1762T / G1764A mutation in 12 chronic (17.64%) and 13 cirrhotic (38.23%) patients. Also, C1766G / G1764T mutations were found in 8.23% of chronic patients and 17.64% of cirrhotic patients.
Conclusion: A1762T / G1764A mutations in the overlapping region of the basal core promoter with gene X C-terminal may lead to liver disease progression from chronic hepatitis to cirrhosis, by changing the amino acid sequence of the X protein.
Esmaeil Samadian, Ayyoob Khosravi , Roghaye Gharae, Mostafa Mir, Seyed Ahmad Sajjadi , Fahimeh Mohammad Abadi, Nader Hashemi, Sahar Alijanpour, Hamid Reza Joshaghani,
Volume 10, Issue 3 (May-Jun 2016 2016)
Abstract
ABSTRACT
Background and Objective: Genetic variations in the gene encoding endothelial nitric oxide synthase (eNOS) enzyme affect the susceptibility to cardiovascular disease. Identification of the way these changes affect eNOS structure and function in laboratory conditions is difficult and time-consuming. Thus, it seems essential to perform bioinformatics studies prior to laboratory studies to find the variants that are more important. This study aimed to predict the damaging effect of changes in the coding region of eNOS using homology- and structure-based algorithms (SIFT and PolyPhen).
Methods: First, the single nucleotide polymorphisms in the coding region (cSNPs) of the human eNOS gene were extracted from dbSNP. Resulting amino acid changes were reported as primary data required for the study. Then, position and type of amino acid changes along with the complete amino acid sequence were separately entered into the SIFT and PolyPhen tools for analysis.
Results: Of 144 single nucleotide changes, 38 changes by the SIFT, 47 changes by the PolyPhen and 18 amino acid substitutions by both tools were predicted as damaging.
Conclusion: It is predicted that 18 amino acid changes may have damaging phenotypic effects on the structure of the eNOS enzyme that may affect its performance by potentially affecting the enzyme’s various functional regions. Therefore, computational prediction of potentially damaging nsSNPs and prioritizing amino acid changes may be useful for investigating protein performance using targeted re-sequencing and gene mutagenesis experiments.
Nourollah Ramroodi , Mohammad Taghi Kardi , Majid Bouzari , Marzieh Rezaei , Majid Komijani , Mahsa Yazdi,
Volume 10, Issue 3 (May-Jun 2016 2016)
Abstract
ABSTRACT
Background and Objective: Herpes simplex encephalitis is a life-threatening consequence of the central nervous system (CNS) infection with Herpes simplex virus (HSV). Although it is a rare disease, mortality rates reach 70% in the absence of therapy and only a minority of individuals can return to normal function. The aim of this study was to determine possible correlation between HSV infection and the incidence of encephalitis in patients with neurological signs.
Methods: Overall, 152 CSF samples were tested from patients with neurological signs referred to Mahdieh Clinical Laboratory in Isfahan from 2010 to 2013. After cerebrospinal fluid (CSF) collection, DNA was extracted and real-time polymerase chain reaction (PCR) was performed for HSV detection.
Results: Of 152 patients tested, 50 were diagnosed with encephalitis. HSV DNA was present in the CSF of 13 patients with encephalitis. HSV was significantly higher (p< 0.05) in patients with encephalitis, which shows the significance of infection as an etiological factor of this disease. About 60% of the encephalitis cases were in age range of 1-24 months.
Conclusion: According to the findings of the present study, Cesarean section is recommended for HSV-positive mothers. A routine real-time PCR test is suggested for HSV detection in patients with encephalitis to avoid unnecessary antiviral treatments.
Masoud Zamani, Maghsoud Peeri, Mohammad-Ali Azarbayjani , Hasan Matinhomaee ,
Volume 10, Issue 4 (Jul-Aug 2016 2016)
Abstract
ABSTRACT
Background and Objective: Adipose tissue secrets various hormones including adiponectin, which is closely related to weight control and energy, balance. This study investigated the effects of resistance training on adiponectin, testosterone and cortisol levels in untrained men.
Methods: Forty untrained men (mean age of 23.8±2.66 years, mean weight of 67.43 ± 4.96 kg) were randomly and equally assigned into groups of upper extremity resistance training, lower extremity resistance training, combined resistance training, and control. The subjects performed eight weeks of weight training, three sessions per week (five sets of 60-85% one repetition maximum). Blood sampling was done prior to the start of the program, after the fourth week, and after the eight week. Alpha level was set to 0.05 for all statically analyses.
Results: Repeated measures ANOVA showed that eight weeks of upper extremities training significantly decreased body fat percentage (p=0.002, 7.39%), and significantly increased adiponectin (p=0.000; 90.42%) and testosterone (p=0.002; 24.19%) levels. In the lower extremities training group, body fat percentage (p=0.006, 7.39%) decreased significantly, while adiponectin (p=0.012; 87.82%) and testosterone (p=0.000; 23.54%) levels increased significantly compared to the pretest. Eight weeks of combined training significantly increased BMI (p=0.006, 1.88%), muscle mass (p=0.007, 2.24%), and adiponectin (p=0.000, 91.56%) level. However, cortisol level decreased (p=0.017, 19.17%) after four weeks of training.
Conclusion: Upper and lower extremities resistance trainings significantly change testosterone levels. Different types of resistance training significantly increases serum adiponectin level and changes body composition, which are effective in prevention of cardiovascular diseases.
Keywords: Resistance Training, Adiponectin, Testosterone, Cortisol.
Seyed Mostafa Mir , Esmaeil Samadian, Sahar Alijanpour , Alireza Khoshbin Khoshnazar , Hamid Haghighatfard, Seyed Hossein Sadeghi,
Volume 10, Issue 5 (Sep-Oct-2016 2016)
Abstract
Background and Objective: The cell division cycle 25 (CDC25)is a familyof highly conserved dual-specificity phosphatases that activate cyclin-dependent kinase complexes. These complexes are the main cell cycle regulators. Mammalian cells ,exposure to DNA damaging radiations such as ionizing radiation and ultraviolet light, prevent cell cycle progression by activation of checkpoint pathways and lead to cell death.
Methods: In this study, mice were exposed to different doses of ionizing radiation. Their total cellular protein was extracted from the bone marrow. After determining and matching the protein concentrations, CDC25A phosphatase levels were measured by western blotting.
Results: The results showed that exposure to different doses of ionizing radiation in vivo significantly increased the expression of CDC25A compared to control group (P <0.05).
Conclusion: Exposure to ionizing radiation increases the expression of CDC25A phosphatase, which increases the possibility of tumorigenesis in that area by increasing bone marrow cell proliferation.
Keywords: Cell Cycle, CDC25A, Ionizing Radiation, Cyclin-Dependent Kinase.
Nazila Hajiahmadi, Abdolvahab Moradi, Naeme Javid, Alijan Tabarraei,
Volume 11, Issue 1 (Jan-Feb- 2017 2017)
Abstract
ABSTRACT
Background and Objectives: Diagnosis of hepatitis E virus (HEV) infection could be missed in some cases if serological tests are used solely. Molecular characterization of HEV is essential for diagnosis of acute and chronic HEV infections, and evaluating the chronic HEV infection status in immunocompromised patients. The aim of this study was to prepare a suitable HEV positive control, determine the limit of detection (LOD) of HEV RNA for a specific molecular test, and evaluate the efficiency and precision of the test.
Methods: Genomic region of HEV NCBI reference sequence was constructed. LOD, intra-assay precision, and inter-assay precision were calculated to evaluate the efficiency and precision of the test. Then, tenfold serial dilutions of the HEV positive control were prepared. Real time PCR was performed three times for each dilution. Mean, standard deviation, and coefficient of variation of cycle thresholds obtained in three independent and simultaneous tests were calculated, and the results were analyzed.
Results: The LOD of this test was determined as 1.4×104 copy/ml or 42 copy/reaction or 14 copy/µl. Intra-assay precision and inter-assay precision for all assays were lower than 2.5% and 10%, respectively.
Conclusion: We propose that the real time PCR assay targeting the ORF2/3 overlapping conserved region is suitable for detection of a wide range of different HEV genotypes found in acute and chronic HEV infections. However, the precision of the test should be improved for detecting HEV RNA lower than 103 copy/ml.
Keywords: Hepatitis E virus, Limit of Detection, Real Time PCR.
