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Attenuation of glucose mediated DNA glycation by Tamarix aphylla extract
Parisa Hasanein , Fahime Javadi Hedaiat Abad, Mousa Bohlooli , Mostafa Khajeh , Sedigheh Esmaielzadeh Bahabadi , Neda Poormolaei ,
Volume 19, Issue 2 (Mar-Apr 2025)
Abstract
Background: DNA glycation, a process where Glc non-enzymatically binds to DNA, is implicated in various detrimental effects, including strand breaks, mutations, and altered gene expression. This damage is considered a significant contributor to the pathogenesis of diabetes mellitus and its associated complications. Consequently, there has been increasing interest in identifying antiglycation agents as a strategy for preventing and mitigating these complications. Prior research has indicated that extracts from Tamarix aphylla (T. aphylla) leaves possess antidiabetic properties. Therefore, this study aimed to investigate, for the first time, the impact of T. aphylla extract on Glc-mediated DNA glycation.
Methods: DNA samples were incubated with Glc over a four-week period. Subsequently, the modulatory effects of T. aphylla on Glc-induced DNA structural alterations were investigated employing a range of analytical techniques. These methodologies encompassed ultraviolet-visible (UV-Vis) spectroscopy, fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and agarose gel electrophoresis.
Results: The results obtained from UV–Vis and fluorescence spectroscopy demonstrated that T. aphylla extract led to a reduction in the formation of DNA-advanced glycation end products (AGEs). Furthermore, CD spectroscopy and agarose gel electrophoresis analyses indicated that the structural alterations of glycated DNA were diminished in the presence of T. aphylla extract.
Conclusion: Based on the evidence presented, T. aphylla demonstrates protective properties against DNA glycation. Consequently, pending further rigorous investigation, it may represent a potentially valuable therapeutic agent for mitigating the detrimental consequences of glycation, particularly in environments characterized by elevated Glc concentrations and hyperglycemic states.

Effects of ethanolic extract of Artemisia Sieberi Besser on DNA glycation of glucose: Possible antidiabetic mechanism
Parisa Hasanein , Mehrnush Sotudeh , Mousa Bohlooli , Mohammad Haddadi,
Volume 19, Issue 6 (Nov-Dec 2025)
Abstract
Background: DNA glycation damages DNA by inducing strand breaks, mutations, and ultimately changes in gene expression, which is considered a main factor in the pathogenesis of diabetes and its complications. Therefore, antiglycation agents have become the focus of recent research for preventing and alleviating diabetes complications. According to the reported antidiabetic effects of Artemisia sieberi (A. sieberi) leaf extract, this study aimed to determine the effect of the ethanolic extract of A. sieberi on glucose-mediated DNA glycation for the first time.
Methods: DNA was incubated with glucose in the presence or absence of A. sieberi for 4 weeks. The inhibitory or facilitatory effects of A. sieberi on DNA structural changes were studied by various techniques. These techniques included UV–Vis, fluorescence spectroscopy, circular dichroism (CD), and agarose gel electrophoresis.
Results: The findings of UV–Vis and fluorescence spectroscopy showed that A. sieberi decreased DNA-AGE (Advanced glycation end products) formation. Based on the CD and agarose gel electrophoresis results, the structural changes of glycated DNA were decreased in the presence of A. sieberi.
Conclusion: Thus, A. sieberi has beneficial effects against DNA glycation and could be a promising agent for ameliorating the adverse effects of glycation in the presence of glucose and in conditions of raised blood glucose, such as diabetes, after confirmation in further studies.
 

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