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Showing 5 results for Hamadi
Seroepidemiology of Helicobacter Pylori infection in Patients With Coronary Syndrome in Gorgan
H Bazzazi, M.a. Ramezani, M Bazoori, A Mohamadi Bondarkheili, M Arabahmadi, A Ghaemi,
Volume 1, Issue 1 (Spring - Summer 2007 [PERSIAN] 2007)
Abstract

Abstract

Background and objectives:

most common causes of morbidity and mortality in industrial and

developing countries. Recent studies have suggested that

A coronary heart disease is one of the

Helicobacter pylori

heart disease therefore, this study was carried out in Gorgan, Iran, to

show the relationship between coronary disease and Helicobacter

pylori infection.

caused infection may be associated with chronic

Materials and Methods:

carried out on 109 patients suffering from acute coronary syndrome

and 85 healthy individuals, ELISA was used to determine Anti

Helicobacter pylori Anti bodies (IgA, IgG ).

In this cross sectional case-control study

Results:

while in control group were %32.9 and %62.4. There was significant

difference between IgA of two groups (p<0.007). Simultaneous

presence of both IgG and IgA in patients affected by Coronary disease

was meaningful (p<0.003).

IgA and IgG antibodies of case group were %51.4 and %53.2

Conclusion

be related to coronary disease, we suggest their investigation in

suspected individuals.

: Since Simultaneous presence of both IgG and IgA may

Key word

Antibody

: Acute Coronary syndrome, Helicobacter Pylori,
Screening of Extended Spectrum Beta lactamase Producing Gram Negative Bacilli Isolated from Clinical Cases
H Hoseinzadegan, A Hassani, M Azadpoor, S Soleimannezhad, F Mohamadi,
Volume 1, Issue 2 (Autumn – Winter 2008[PERSIAN] 2007)
Abstract

Abstract

Background and objectives:

(ESBL) strain is one of the emerging health related problems in the world recently.

Some of the species of the gram-negative bacilli including Klebsiella Pneumonia &

Escherichia Coli are well known ESBL producing among bacteria, and they cause

uncontrollable infections. This Cross-sectional study was designed to asses the

ESBL producing gram negative bacilli among inpatients of Shohada-ye- ashayer

hospital (Khorram Abad).

Extended Spectrum Betalactamase producing

Materials and methods:

methods. ESBL producing gram negative bacilli were screened with MacConkey

Agars containing 4 mg/liter Ceftazidime and confirmed with double disk synergy

method as recommended by national standard laboratory institute.

Samples were processed with routine laboratory

Results:

positive for ESBL.The most isolated species of ESBL are 20 Klebsiella

pneumonia(8.88%), 10 Escherchiia coli(4.44%) and 10 pseudomonas

aeruginosa(4.44%). The most ESBL producing gram-negative bacilli were Isolated

from urine samples (21 cases 39.62%).and Ten cases (18.86%) from bronchoscopy

sterile samples.

Fifty- there cases (23.55%) of 225 total isolated gram negative bacilli are

Conclusion:

frequently isolated from Shohada-ye-Ashaier Hospital. Regarding the high

resistance of these strains against many of the antibiotics and even against

Carbapenems, health- care providers need to plan controlling policies for such

strains.

The Results indicate that ESBL producing gram-negative bacilli are

Key words:

 

Extended Spectrum Betalactamase.

Hospital acquired infection, Escherichia coli, Klebsiella Pneumoniae,
Prostatic massage method versus first -void Urine samples to isolate Mycoplasma hominis and Ureaplasma urealyticum from urinary tract infection
K Ghazisaidi, F Fateminasab, Sh Vatani, Y Azimi, S Bakhshandeh Nosrat, M Mohamadi,
Volume 2, Issue 1 (Spring - Summer 2008[PERSIAN] 2008)
Abstract
Abstract Background and objectives: Ureaplasma urealyticum is one of the most important causes of Nongonococcal urethritis (NGU) and non-specific urethritis (NSU) in men. Also Mycoplasma hominis has a role in causing NGU and NSU. The aim of this study was the detection of Mycoplasma hominis & Ureaplasma urealyticum in men suffering from NGU and NSU by prostatic massage method and first- void Urine samples. Material and Methods: Urethral secretion samples, taken after prostatic massage and first-void urine, of 75 patients referred to department of bacteriology of Tehran University of Medical Sciences were examined for ureaplasma and mycoplasma by culture method in 2004-2005 Results: In prostatic samples, 19 and 11 samples are positive for ureaplasma and Mycoplasma, retrospectively. In first-void urine samples, 17 and 9 samples are positive for ureaplasma and Mycoplasma, retrospectively. Conclusion: The results show that if it is impossible to obtain urethral sample via prostatic massage , we can use first- void urine instead. Key words:Mycoplasma hominis, Ureaplasma urealyticum,Nongonococcal urethritis, Nonspecific urethritis, prostatic massage, first voided Urine
Expression Analysis of Cellular Mir-29a and mir-29b in HIV Positive Patients
Hasan Kaleji, Alijan Tabaraei, Abdollah Abbasi, Naemeh Javeed , Masoud Bazoori , Reza Golmohamadi , Abdolvahab Moradi,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract

Abstract

       Background and Objective: Various cellular factors affect the process of HIV activity. One of these cellular factors are structures known as microRN that are expected to be involved in controlling HIV replication and infectivity. The expression of one or a set of them may represent the patient's clinical conditions. In this study,  the expression of miR-29a and miR-29b involved in regulating viral genes’ expression was evaluated in three HIV-positive groups and a healthy control group. Later,  the expression level of these microRNAs  was compared between the cases and controls.

      Methods: Total RNA extraction was performed on the collected samples using RNx-plus kit and then the microRNA expression levels were evaluated using Relative Real-time PCR. The obtained data was entered into SPSS 22 and Graphpad softwares and analyzed using Kruskal-Wallis and Man-Whitney tests. P-value of less than 0.05 was considered as statistical significance level.

     Results: The expression level of miR-29a  was reduced in patients under treatment and drug-resistant patients ( P ≤ 0.05)  . All three HIV-positive groups including people without drug treatment, patients under treatment and drug-resistant patients showed reduced miR-29b expression level compared to  control group (P ≤ 0.05).

     Conclusion: the decreased expression of miR-29a and miR-29b in patients under treatment and drug-resistant patients indicates an  increased viral replication and reduced CD4 cell count. It may be possible to predict the progression of the disease by miRNA measurement or control viral replication using these mir-RNAs that requires further studies.

        Keywords: HIV, expression, mir-29a, mir-29b.


Applicability of Saliva for Evaluation of Some Biochemical Parameters of Kidney and Liver Function in Healthy Individuals
Nasir Idkaidek, Hiba Qawasmi, Alaa Hanahen, Luay Abuqatouseh, Salim Hamadi, Mona Bustami,
Volume 14, Issue 4 (Jul-Aug 2020)
Abstract
ABSTRACT
          Background and Objectives: Proper diagnosis of clinical conditions is a major goal of clinical and biochemical analyses. Recently, increasing efforts have been put on the use of less invasive sampling techniques with optimal sensitivity and specificity. The aim of this study was to investigate the applicability of saliva instead of blood for measuring biochemical parameters of liver and kidney function in healthy individuals.
          Methods: Plasma and saliva samples were collected from 100 healthy volunteers to measure level of alkaline phosphatase (ALP), alanine transaminase (ALT), aspartate aminotransferase (AST), total bilirubin, gamma-glutamyl transpeptidase (GGT), urea and creatinine using a fully automated chemistry analyzer (ACE Alera) with ready to use validated kits. Receiver operating characteristic (ROC) analysis was carried out using MediCal program to calculate sensitivity and specificity and area under ROC (AUC).
          Results: The mean (standard deviation) salivary level of ALP, AST, ALT, GGT, total bilirubin, creatinine and urea was 20.9 (20.7) U/L, 25.8 (17.9) U/L, 10.6 (11.8) U/L, 9.6 (4.37) U/L, 0.16 (0.13) mg/dL, 0.09 (0.05) mg/dL and 35.6 (15.2) mg/dL, respectively. Saliva to blood ratios of ALP, AST, ALT, GGT, total bilirubin, creatinine and urea was 14%, 113%, 65%, 45%, 19%, 12% and 130%, respectively. The suggested normal saliva ranges of ALP, AST, ALT, GGT, total bilirubin, creatinine and urea were 7-98 (U/L), 31-104 (U/L), 6-31 (U/L), 15-24 (U/L), 0-0.13 (mg/ dL), 0.14-0.31 (mg/ dL) and 45-74 (mg/ dL), respectively.  The calculated sensitivity and specificity values were 38%  and 85% for ALP), 80% and 76% for AST, 75% and 45% for ALT, 60%  91% for GGT, 49% and 38% for total bilirubin, 20% and 91% for creatinine and 100% and 75% for urea. The AUC was higher than 0.7 for urea, GGT and AST, indicating good sensitivity and specificity of saliva testing for evaluation of these enzymes.
          Conclusion: Based on the results, saliva could be as a noninvasive method of assessing kidney and liver function. Saliva may be a favorable alternative to plasma for measuring level of urea, GGT and AST in humans.

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