Z Gharibi, F Daadras, M Maghsood, M Fallah,, M Saeedijam,
Volume 7, Issue 4 (winter[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: Microsporidia is an obligatory intracellular parasite known as an opportunistic infection in immunocompromised patients. Its laboratory diagnosis is relatively difficult and modified trichrome staining is a standard diagnostic method for detection of Microsporidia. The aim of present study was to identify intestinal Microsporidia in kidney- transplanted patients, using modified Trichrome Staining and Calcofluor White Methods.
Material and Methods: In 2012, 180 stool specimens were taken from kidney transplanted- patients given immunosuppressive drugs in Hamadan, Iran. To identify Microsporidia we use modified Trichrome Staining and Calcofluor White Methods.
Results: The mean duration of kidney transplant and immunosuppressive drug using was 5.5 Years. Only one female patient was positive for Microsporidia.
Conclusions: Owing to low frequency of this opportunistic infection among kidney transplanted patients, we can conclude that their hygienic conditions are good enough and they are not exposed with the parasites.
Key words:Microsporidia, Modified Trichrome Staining,Kidney Transplant, Calcofluor White, Hamadan
Abdol Kazem Neisi, Huria Gharibi, Afshin Takdastan, Hamideh Rezazadeh, Mina Badiee, Fatemeh Zohrehvand, Adel Nazarzadeh,
Volume 11, Issue 2 (Mar-Apr 2017)
Abstract
ABSTRACT
Background and Objective: Mycotoxins are a group of relatively resistant toxic metabolites. The most important mycotoxins are aflatoxins (B1 and B2, G1 and G2), which originate from contaminated animal feed. Dairy cattle transmit aflatoxins B1 and B2 through milk in form of aflatoxins M1 and M2, and endanger the human health. Traditional buffalo farms play an important role in the supply of dairy products in Khuzestan Province. In addition, the province has suitable conditions for the growth of various types of fungi. Therefore, this study aimed to determine the amount of aflatoxins in buffalo feed samples collected from two main suppliers of milk in the province (Ahvaz and Karun).
Methods: Overall, 60 samples were collected during the 3 months of autumn 2014. Samples were analyzed by the sensitive and fast method of competitive enzyme-linked immunosorbent assay.
Results: The concentration of aflatoxin ranged from 0.77 to 64.85 μg/Kg. In addition, the concentration of aflatoxin in 21 samples was higher than the permitted limit (25 μg/Kg).
Conclusion: The mean concentration of aflatoxin in the samples increases with the decrease in temperature and humidity. This increase is observed in Ahvaz at a higher rate, which could be due to inappropriate storage and use of rice bran in their feed.
Keywords: Aflatoxins, Ahvaz, Animal Feed.
