Showing 10 results for Ghane
Sh Shargh, M A Yeghaneh, Sm Mohades, A A Ayetollahi, A Khalaj, A Khandan Del,
Volume 4, Issue 1 (Spring - Summer 2010[PERSIAN] 2010)
Abstract
Spring summer 2010, Vol.4, No.1 /72 Medical Laboratory Journal Evaluation of Cholesterol Panel Changes in Fish Consumers in the West of Mazandaran Province, Iran Abstract Bachground and objectives: Eating fish reduces low-density lipoprotein cholesterol (LDL-C) and increases high density lipoprotein cholesterol (HDLC). Because of different factors, such as physiological conditions and kind of fish consumption, the findings can be different. We decided to investigate the reducing effect of regular fish eating on plasma lipids and lipoproteins of different groups. Material and Methods: the Subjects were 50 clients (control group) with normal Lipid and 50 ones with high Lipid (case group). The subjects, with different sex and age, were asked about smocking, heart disease and diabetes. Fast blood samples were collected and analyzed for total cholesterol (TC), low and high density lipoprotein cholesterol (LDL-C, HDL-C and VLDL), TG, HDLC, apolipoprotein A and B. Results: The data shows a significant difference in cholesterol, LDL, apoA and VLDL levels in moderate and high consumers. (P<0.005). In subjects with high LDL, the kind of fish consumption was stir- fried (0.96%) and grilled and boiled (7.4%). The Subjects with high-fish consumption is 32.2% for normal LDL Level and Just 1.1% for ab normal Level. There is no significant effect on HDL and apoB levels due to fish intake in any dosage. The relation is seen between abnormal lipid and rare fish consumption. Conclusion: Present study shows the reducing effect of fish consumption on cholesterol level and LDL-C. More studies are needed to be conducted to evaluate the type of faty acids in fishes. . Key words: Fish, dietary, cholesterol panel. Shargh SH FacultyMember of Dept of Laboratory Sciences, Islamic Azad University, Chaloos Branch Yeghaneh MA FacultyMember of Dept of Laboratory Sciences, Islamic Azad University, Chaloos Branch Mohades SM (PhD) Assistant Professor of Health Faculty, Tabriz University of Medical Sciences Ayetollahi AA (MD) FacultyMember of Dept of Lab Sciences, Paramedical Faculty, Golestan University of Medical Sciences Khalaj E (BSc) Daghigh Clinical Laboratory Khandan Del A Laboratory Technician, Islamic Azad university,Chaloos Braneh Corresponding: Shargh, SH Email:shohrehshargh@gmail.com
Sz Azimi, M Ghane, Z Heshmatipour,
Volume 7, Issue 2 (summer[PERSIAN] 2013)
Abstract
Abstract: Background and Objective: A wide variety of opportunistic pathogens has been detected in hospital surfaces. Among these , Pseudomonas species are one of the leading causes of nosocomial infections, frequently found in hospital environments. The purpose of this study was identification of antimicrobial susceptibility of Pseudomonas spp. isolated from different Section of ShahidRajaeii hospital, Tonekabon. Material and Methods: the samples (460) from different sections of Shahid Rajaeii hospital, Tonekabon were collected between December 2010 and June 2011. The identification of the strains was performed by using biochemical tests and API20NE (Biomerieux) ,and antimicrobial susceptibility of the isolates against different antibiotics was determined by disc diffusion test. Results: of 460, 61(%13/26) strains of Pseudomonas are isolated from all the sources. The highest rate of Pseudomonas spp. is recorded in Surgery and ICU, while the lowest in Dialysis ward. Of 61 strains of Pseudomonas, 52 (85/25%) are belonged to Pseudomonas aeruginosa, six (9/83%) to Pseudomonas stutzeri, two (3/28%) to Pseudomonas putida and one (1/64%) to Pseudomonas fluorescens. Conclusion: the environments of the hospital can be the vehicles of Pseudomonas spp. therefore, both the patients and personnel should have extra attention to their personal hygiene to avoid Pseudomonas infection. Keywords: Nosocomial Infections Pseudomonas Antibiotic Susceptibility
H Bashi Zadeh Fakhar, R Faraji, M Ghane, M Jafarpour, B Ashoorizadeh,
Volume 7, Issue 3 (Autumn 2013)
Abstract
Abstract
Background and Objective: Much research has shown that Human Papiloma Virus (HPV) plays an important role in cervix cancer and it is the cause of 99% of cervix cancer worldwide. Lots of research has been done to find a proper method for HPV diagnosis and screening in patients with genital warts. This study aimed at comparing PCR method with Pap smear test in HPV screening.
Material and Methods: Considering the presence of DNA of HPV, 45 vaginal and cervix swap samples of women with genital warts were tested by means of specific PCR and Pap smear from September 2010 to April 2011.
Results: Out of 45 vaginal and cervix swap samples of women suffering genital warts, 37 samples (82.2%) are positive. Of 45 Pap smear samples, 13 (29%) are neoplasia and 32 (71%) normal.
Conclusion: The difference between the results of PCR and Pap smear is due to low specification and sensitivity of Pap smear. Thus it is recommended using diagnostic PCR method in addition to Pap smear in order to promote the quality of screening in individuals with genital warts.
Keywords: Human Papiloma Virus (HPV) Genital Warts Molecular (PCR) Pap Smear
Naghdi, N, Ghane, M,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract
Background and Objective: Propionibacterium acne is one of the main causes of acne. Due to the spread of drug resistance, it is not responsive to treatment. This study aimed to determine antibiotic sensitivity of strains of the Propionibacterium acne.
Material and Methods: seventy samples of acne lesions were collected to study the presence of Propionibacterium acne. Microbial Culture technique was used to detect and identify Propionibacterium acne. Antibiotic resistance of the isolates to the antibiotics of Doxycyclin, Azithromycin, Erythromycin, Tetracycline, Clindamycin and Trimethoprim sulfamethoxazole was studied by Antibiogram method.
Results: Of 70 samples, 14 (20%) were positive for Propionibacterium acne. The results of phenotypic test were confirmed using molecular method. Rate of resistance to Azithromycin and Erythromycin (50%), Clindamycin (35.71%), Trimethoprim sulfamethoxazole (28.57%), Doxycycline and Tetracycline (14.29%) was determined.
Conclusion: Outbreak of antibiotic resistance to Azithromycin, Erythromycin, and Clindamycin is high. Since the Propionibacterium acne is sensitive to Doxycycline and Trimethoprim Sulfamethoxazole, it is recommended using them to treat acne.
Keywords: Antibiotic susceptibility, Propionibacterium Acne, Ance Protein.
Masood Ghane ,
Volume 12, Issue 1 (Jan-Feb 2018)
Abstract
ABSTRACT
Background and Objectives: Previous studies have demonstrated the relationship between viral infections and risk of developing type 1 diabetes. The aim of this study was to investigate the frequency of Herpes simplex virus (HSV) in patients with type 2 diabetes and healthy control individuals using PCR and ELISA.
Methods: Blood samples were taken from 180 diabetic patients and 187 healthy controls referred to the Pasteur medical laboratory in Tonekabon, in 2016. Human beta-globin gene was used as internal control to ensure extraction accuracy. Specific primers were used for amplification of the UL30 gene. In addition, level of anti-HSV IgG antibody was measured using a commercial ELISA kit (Euroimmun, Germany).
Results: DNA of HSV was found in the samples of 11 patients (6.1%) and five healthy controls (2.7%). In addition, anti-HSV IgG was found in the samples of 117 patients (65%) and 108 healthy controls (57.75%). There was a statistically significant relationship between frequency of anti-HSV IgG and diabetes.
Conclusion: Similar to previous studies, the present study demonstrated a relationship between frequency of HSV infection and type 2 diabetes. However, further studies should be performed to eliminate the effect of other risk factors to help clarify the exact role of viral infections in increasing the risk of diabetes.
Keywords: Diabetes, Herpes Simplex Virus, ELISA, PCR.
Shadi Beladi Ghannadi , Maryam Ghane , Laleh Babaeekhou ,
Volume 13, Issue 2 (Mar-Apr 2019)
Abstract
ABSTRACT
Background and Objectives: The emergence of extended-spectrum β-lactamase (ESBL)-producing Shigella spp. is becoming a health concern worldwide. This study aimed to investigate antibiotic resistance pattern and frequency of blaCTX-M, blaSHV, and blaTEM genes among Shigella isolates from patients in hospitals of Tehran, Iran.
Methods: In this cross-sectional study, 52 non-repeated Shigella strains were isolated from hospitalized patients in Milad, Emam Khomeini and Shariati hospitals in Tehran (Iran) from November 2015 to December 2016. Bacterial identification, serotyping, and antimicrobial susceptibility testing were performed according to the standard guidelines. The blaCTX-M, blaSHV, and blaTEM resistance genes were identified using multiplex polymerase chain reaction.
Results: Among 52 Shigella isolates, S. sonnei (44.2%) was the predominant species, followed by S. flexneri and S. dysenteriae (23%). Over 67% of the isolates were multidrug resistant. The highest rates of resistance were observed against cefalotin (67.3%), tetracycline (67.3%), amikacin (63.5%), trimethoprim-sulphamethoxazole (48.1), and ampicillin (42.3%). The lowest resistance rate was against ciprofloxacin (1.9%). We detected the blaTEM and blaCTX-M genes in 61.5% and 19.2% of the isolates, respectively. However, the blaSHV gene was not detected in any of the isolates. In addition, 16.4% of the isolates harbored the blaTEM and blaCTX-M genes simultaneously. Ciprofloxacin was the most effective antibiotics according to the ESBL genes distribution.
Conclusion: Our findings indicate the high prevalence of multidrug resistance and ESBL genes in Shigella isolates, which elucidates the need for appropriate infection control measures for limiting the spread of resistant strains.
Keywords: Shigella, Multiplex Polymerase Chain Reaction, Drug Resistance.
Seyed Amin Enayatzadeh Meymandi, Laleh Babaeekhou, Maryam Ghane,
Volume 13, Issue 5 (Sep-Oct 2019)
Abstract
ABSTRACT
Background and Objectives: Emergence and spread of multidrug-resistant (MDR) and extensively-drug resistant (XDR) Pseudomonas aeruginosa strains could complicate antipseudomonal chemotherapy. Dissemination of resistance genes, such as β-lactamases encoding genes by horizontal gene transfer can lead to development of multi-drug resistance in P. aeruginosa. The purpose of this study was to investigate the latest resistance patterns in MDR and XDR strains and evaluate Ambler class A β-lactamase gene distribution in P. aeruginosa clinical isolates.
Methods: One hundred molecularly and biochemically identified P. aeruginosa strains isolated from different clinical specimens were tested for sensitivity to 17 antibiotics using the Kirby-Bauer disk diffusion method. PCR was performed to detect bla TEM-1, bla SHV-1, bla REP-1 and bla VEB-1 genes. Results were analyzed using SPSS and NTSYSpc softwares.
Results: Based on the results of antibiogram, the highest rate of resistance was observed against amikacin (100%), aztreonam (83%), ceftazidime (55%), cefepime (55%) and netilmicin (48%). In addition, the frequency of MDR and XDR isolates was 95% and 5%, respectively. The blaSHV-1, bla TEM-1, bla PER-1 and bla VEB-1 genes were detected in 31%, 24%, 13% and 10% of the isolates, respectively.
Conclusion: Antibiotic resistance to β-lactam antibiotics and frequency of β-lactamase genes were relatively high in the study area. We also found that a significant proportion of XDR strains with different antibiotic resistance profile is isolated from tracheal specimens.
KEYWORDS: Pseudomonas aeruginosa, Beta-Lactamase, Multidrug Resistant, Extensively Drug Resistant.
Mina Eghbali, Majid Baserisalehi, Masood Ghane,
Volume 14, Issue 3 (May-Jun 2020)
Abstract
Background and Objectives: Moraxella catarrhalis is considered as an emerging pathogen and a new nosocomial infection agent. This study was conducted to isolate and identify M. catarrhalis from clinical samples (respiratory tracts) and assess them for antimicrobial susceptibility patterns.
Methods: In total, 280 samples were collected from patients with respiratory tract infection, and 120 samples were obtained from healthy individuals in the control group. The isolates were identified by phenotyping and genotyping methods, and their antibiotic susceptibility was evaluated using disk diffusion methods. The presence of β-lactamase and efflux pump activity were specified via phenotypic methods. Finally, Bro and acrA genes in the isolates were detected by PCR technique.
Results: The frequency of this bacterium was 9.64% (27 out of 280) in patients with respiratory tract infection and 4.16% (5 out of 120) in the control group. Although the isolates were resistant to penicillin, they had various responses against other antibiotics. The results obtained from molecular method showed that 90.6% and 84.3% of the isolates possessed Bro and acrA genes, respectively. There was a significant relationship (P<0.05) between the presence of Bro and acrA genes and antibacterial resistance to ampicillin, amoxicillin, cefazolin, cefuroxime, and chloramphenicol.
Conclusion: Our findings confirmed the existence of M. catarrhalis in patients with respiratory diseases and the high prevalence of antibiotic resistant genes in M. catarrhalis isolates. Therefore, timely diagnosis and successful treatment can play important roles in preventing their spread.
Sona Rostampour Yasouri, Masoud Ghane, Monir Doudi, Abolhasan Rezaee, Nafiseh Sadat Naghavi,
Volume 14, Issue 6 (Nov-Dec 2020)
Abstract
Leptospirosis is a zoonotic disease with a high incidence rate in many parts of the world due to the presence of various hosts for the pathogenic Leptospira. Tropical, subtropical and humid regions are suitable for long-term survival of the bacterium. Because of the temperate and humid climate, northern areas of Iran are suitable for pathogenic Leptospira and outbreak of the disease. Therefore, identification of infected areas is important from a public health and economic point of view. Previous studies show that the incidence rate of leptospirosis is increasing every year. Therefore, accurate diagnosis, control and prevention of this disease seem necessary through vaccination and raising public awareness, especially among high-risk groups. Today, diagnostic methods including immunofluorescence assay, enzyme-linked immunosorbent assay, microscopic agglutination test (MAT) and polymerase chain reaction (PCR) are used to diagnose the leptospirosis. MAT is the gold standard test for the diagnosis of leptospirosis with extensive applications in Iran. Due to the importance of this disease and its high prevalence in recent years, the present study aimed to investigate the epidemiology and diagnosis of leptospirosis in Iran.
Farnaz Zanghaneh, Parvin Farzanegi, Habib Asgharpour,
Volume 16, Issue 1 (Jan-Feb 2022)
Abstract
Background and objectives: Programmed autophagy is a genetically and evolutionarily conserved process that destroys long-lived cellular proteins and organelles. This study aimed to investigate effects of continuous and interval exercise training with or without atorvastatin supplementation on Beclin1, LC3-I and LC3-П expression in old rats with type 2 diabetes.
Methods: Sixty three male Wistar rats were divided into eight groups. Continuous exercise was performed at a speed of 15-29 m/min for 5-22 minutes. Interval exercise program consisted of six 2.5-minute sets that included a four-minute rest period between each set. The rats in the supplementation groups also received 20 mg/kg body weight atorvastatin daily via intraperitoneal injection. At the end of the training period, the expression of Beclin1, LC3-I and LC3-П in soleus muscle was measured by RT-PCR. One-way ANOVA was used for data analysis at statistical significance of 0.05.
Results: The results showed that both exercise trainings with or without atorvastatin significantly reduced LC3I, LC3-II and Beclin1 compared with the diabetic control group (P<0.05). In addition, the effects of the trainings and atorvastatin supplement did not differ significantly (P>0.05).
Conclusion: The results indicate that continuous and interval exercise program alone and combined with atorvastatin supplementation could significantly reduce LC3-1, LC3-II and Beclin1 level in soleus muscle of old diabetic rats.
