M Rasouli Nasab, Sh Habibnia, P Heidarieh, Mr Pourmand, M Fatahi, Ss Eshraghi,
Volume 7, Issue 5 (supplement Issue( Bacteriology)[PERSIAN] 2014)
Abstract
Abstract
Background and Objective: The Isolation of Nocardia species is complex and time-consuming, which is due to rapid growth of adjacent bacteria. Because of the importance of a specific medium with the ability of controlling intrusive microorganisms, this study aimed at comparing three laboratory methods to introduce the reliable isolation technique for Nocardia species.
Material and Methods: The soil samples were collected from different regions of Tehran province, Iran, and carefully transferred to the laboratory. The samples were cultured in three different media including Paraffin Baiting,Humic acid vitamin B agar and Paraffin agar, and incubated for 3-4 weeks at 35 °C.
Results: Of 110 soil samples, 31 Nocardia isolates (28.18%) were obtained from the media including Paraffin Baiting, (19 17.27%), Humic acid and vitamin B agar (4 3.63%), and Paraffin agar, (8 7.27%).
Conclusion: because of high rate of isolation, low cost and the clearance of colonies suspected nocardia, Paraffin Bait technique is more reliable and efficient compared to the other methods.
Key words: Nocardia Soil Paraffin Baiting Humic Acid Vitamin B
Mohammad-Ali Mohaghegh, Roghayeh Norouzi, Abolghasem Siyadatpanah, Farzaneh Mirzaei, Ali Fatahi Bafghi, Seyed-Reza Mirbadie,
Volume 15, Issue 3 (May-Jun 2021)
Abstract
Background and objectives: Soil contamination with Toxocara spp. eggs is the main source of human toxocariasis. The aim of this study was to determine rate of soil contamination with Toxocara spp. eggs in Yazd, central Iran.
Methods: During September 2014 to February 2015, a total of 220 soil samples were collected from public parks, primary schools, green areas, kindergartens, suburban areas, streets, residential complexes, backyards and a bus terminal in the city of Yazd, Yazd Province. Soil samples were investigated for the presences of Toxocara eggs by flotation technique using sucrose solution (1.2 g/cm3) and using light microscopy. Data analysis was carried out by SPSS (version 23) software package.
Results: A total of 220 soil samples from nine different places were examined. Of these samples, 51 (23.2%) were contaminated with Toxocara spp. eggs. The contamination rate was 26.3% in public parks, 19.2% in primary schools, 28.6% in green areas, 22.2% in kindergartens, 24% in suburban areas, 8.3% in streets, 18.8% in residential complexes, 19% in backyards and 28.6% in the bus terminal. There was no significant difference in the soil contamination rate with Toxocara spp. eggs between different sampling places (χ2 = 3.096, d.f = 8 and P= 0.92) and sampling areas (χ2= 2.289, d.f = 2 and P= 0.318).
Conclusion: The high rate of soil contamination with Toxocara spp. eggs in Yazd emphasize on the need for preventive measures, such as improving sanitation, providing public health education and controlling stray dogs and cats near children play areas.
