Showing 5 results for Eslami
M Mosayebi, B Ghorbanzadeh, Z Eslamirad, M Ejtehadifar, B Rastad,
Volume 7, Issue 4 (winter[PERSIAN] 2014)
Abstract
Abstract Background and Objective: Acanthamoeba species are free-living protozoa that can be isolated from all environments. They can bring about different diseases in healthy individuals and immune suppressed patients, for example, Granulomatous Amoebic Encephalitis (GAE), Acanthamoeba Keratitis (AK), Cutaneous and Nasopharyngeal infections. The aim of this study was to evaluate the Acanthamoeba prevalence in rural water sources of Markazi province. Material and Methods: In this cross sectional study, 54 water samples were collected from 36 villages of Markus province. First, the Samples were filtered by filter paper (watchman 42). Next, the filtered paper were placed in page saline solution and centrifuged. Then, the obtained sediment was cultured on non-nutrient agar (NNA) plates overlaid with heat-killed Escherichia coli. After that, the provided smear (after 4 - 7 days) stained with Geimsa. Results: The samples were positive (33 61.11%) and negative (21 38.89%) for Acanthamoeba cyst. The best result for isolation of Acanthamoeba cysts was obtained after shaking of filter paper. Conclusion: A high percentage of rural water sources have been contaminated with Acanthamoeba, which can be the major factor in causing human infections. Therefore, some effective methods are required to prevent from water sources contamination. Keywords: Acanthamoeba Acanthamoeba Keratitis Markazi Province Rural Water Sources
Mohajerani, Mr, Sarikhani, A, Gandomani, M, Eslamirad, Z, Mosayebi, M, Didehdar, M,
Volume 8, Issue 5 (winter[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Malassezia that is a part of normal flora is lipophilic yeast involved in a variety of skin diseases such as seborrheic dermatitis, pityriasis versicolor, atopic dermatitis and psoriasis. Seborrheic dermatitis affects most often the sebaceous-gland-rich areas of skin such as face, scalp, and parts of the upper trunk. Dandruff is a mild variant of seborrheic dermatitis characterized by scaling. In this study, Malassezia species causing dandruff were identified. Material and Methods: In this descriptive study, the samples (n= 60) from participants with dandruff were examined under a microscope using 10% KOH solution and cultured in Leeming and Notman ager medium. DNA Extraction was performed from colonies by glass bead and the Malassezia genus, and species were detected by CfoI enzyme using PCR-RFLP method Results: Of 60, 40 (66.6%) were positive for Malassezia yeast. The positive samples in direct examination grew in culture medium. Malassezia species isolated were Malassezia globosa (25 cases), Malassezia restricta (10 cases), Malassezia furfur (3 cases) and Malassezia sympodialis (2 cases). Conclusions: In most studies, the Malassezia species were identified as the agents causing seborrheic dermatitis. In our study, Malassezia globosa was isolated as a dominant species. Keywords: Seborrheic Dermatitis, Malassezia SPP, Arak
Shirin Sheikholeslami , Seyed Mahdi Rezayat , Reza Hosseini Doust , Hamid Reza Ahmadi Ashtiani ,
Volume 10, Issue 1 (Jan,Feb 2016 2016)
Abstract
Abstract
Background and Objective: The spread of drug resistance in bacteria have prompted researchers to seek suitable alternative for antimicrobial drugs among various medicinal plants and nanoparticles. The aim of this study was to evaluate the effect of silver nanoparticles alone and in combination with methanol extract of Zataria multiflora on five Gram-positive and Gram-negative bacteria.
Methods: Different concentrations of the nanoparticles and extract alone or in combination with each other were tested against the bacteria, using well diffusion method. Three concentration levels (lowest, average and highest) were prepared form the nanoparticles and the extract for the combination, and finally nine different combinations were prepared.
Results: The extract and nanoparticles showed inhibitory effects against all the tested bacteria. The maximum diameter of growth inhibition zone in the presence of the extract and nanoparticles were observed in Streptococcus pyogenes (35.6mm) and methicillin-resistant Staphylococcus aureus (20.6mm), respectively. The maximum diameter of growth inhibition zone for the combination was measured in S. pyogenes (31mm).
Conclusion: The combination of low concentrations of the plant extract and nanoparticles are more effective against bacteria, but the combination of their high concentrations reduce the antibacterial effects in some cases.
Zahra Eslami, Yahya Mohammadnajad Panah Kandi, Alireza Norouzi, Abdorreza Eghbal Moghanlou, Mehdi Sheikh Arabi, Vahideh Kazeminejad, Seyedeh Somayeh Hosseini Alarzi, Aref Saeidi, Hamidreza Joshaghani,
Volume 16, Issue 3 (May-Jun 2022)
Abstract
Background and objectives: Non-alcoholic fatty liver disease (NAFLD) is a chronic liver disease caused by the accumulation of large amounts of fat in the hepatocytes. Given that atorvastatin is effective for treatment of NAFLD, the present study investigated effects of high-fat/fructose diet (HFFD) with atorvastatin on liver enzymes and lipid profile in a NAFLD rat model.
Methods: Thirty-two male Wistar rats were divided into four groups: 1) normal control, 2) HFFD control, 3) HFFD + atorvastatin, and 4) normal + atorvastatin. The groups received HFFD for 15 weeks to induce hepatosteatosis. Atorvastatin was administrated at the dose of 10 mg/kg/day. Lipid profile and liver enzymes were measured after eight weeks of intervention.
Results: Triglyceride, cholesterol, gamma-glutamyl transferase, and aspartate transaminase were significantly reduced in the HFFD + atorvastatin group compared with the HFFD control group. In addition, cholesterol, high-density lipoprotein, alkaline phosphatase, and gamma-glutamyl transferase were significantly increased in the normal + atorvastatin group compared with the normal control group. Low-density lipoprotein increased significantly in the HFFD + atorvastatin group and the normal + atorvastatin group compared with other groups. There was a significant difference in the alanine transaminase levels between the groups taking atorvastatin. In fact, alanine transaminase level was lowest in the normal + atorvastatin group.
Conclusion: Atorvastatin improves the lipid profile and fatty liver and controls liver enzymes. Therefore, it can be used with caution to improve the lipid profile and reduce the complications of NAFLD.
Zahra Eslami, Shayan Marhamaty, Seyyed Mehdi Jafari, Mohadese Khorasani, Mehdi Sheikh Arabi, Hamidreza Joshaghani,
Volume 19, Issue 1 (4-2025)
Abstract
background:
Bivalent minerals have an important role as cofactors which play vital roles in various metabolic pathways in the body. Zinc (Zn) has catalytic, structural, and regulatory roles. Severe Zn deficiency may cause the abnormal synthesis of nucleic acids, and proteins, impaired cellular growth, excessive cell death, and excess lipid peroxidation of the cellular membrane that is associated with shortening the RBC lifespan.
Purpose:
The aim of this study was to examine the associations between Zn status and the erythrocyte indices.
Methods and materials:
A number of 563 individuals (72.8% female) were included in this study. The level of serum Zn was measured by photometric method and blood index values were measured by using a cell counter.
Results:
The average serum Zn level is 102.8± 17.6 mg/dl. Serum Zn level is directly related with RBC (R=0.119, PV=0.005) and MCHC (R=0.086, PV=0.041) but it is inversely related with MCV (R=-0.097, PV=0.021). These results also determined that serum Zn level, as well as the levels of RBC, Hb, HCT, and MCHC, were significantly higher in men (Sig<0.01) but the level of MCV among women was higher (Sig<0.01). Moreover, in individuals with <30 serum Zn level, MCHC (Sig<0.01), and RBC (Sig<0.05) were higher whereas Hb (Sig<0.05), HCT, MCV, and MCH (Sig<0.01) were higher at >30.
Conclusion:
According to the positive relationship between Zn level and RBC, Zn deficiency affects the number of RBCs.
