Showing 7 results for Bakhshi
F Ghasemi Kebria, B Khodabakhshi, H Kouhsari, M Sadeghi Sheshpoli, N Behnampoor, S Livani, M Bazuori, E A Ghaemi,
Volume 4, Issue 1 (Spring - Summer 2010[PERSIAN] 2010)
Abstract
Abstract Background and objectives: After respiratory infection, Diarrhea is the second cause of mortality. Yersinia enterocolitica is the second important cause of infectious diarrhea in children of some countries. In this study, we evaluated the frequency of Yersinia entocolitica of diarrheal specimens in Gorgan, Iran. Material and Methods: This descriptive cross - sectional Study was carried out on diarrheal stools of 455 patients referred to medical centers and laboratory of Gorgan in 2004-2005. DNA extraction using phenol chloroform was performed for all samples. Using two specific primers (genus-specific16s rRNA and ail- specific species genus of Yersinia enterocolitica), we did PCR sample. Results: Yersinia genome was identified in 12 patients(2.63%) and 11 of them was Yersinia enterocolitica. The frequency infection in of girls (3%) was more than boys (2.4%), and the prevalence in winter (4%) was more them other seasons, and under one- year- group (3.4%) and 1-5 years (3.1%) is more than other age groups. It was not observed significant difference. (P> 0.05). Conclusion: The frequency of Yersinia in cases of diarrhea in Gorgan is similar to most regions of Iran and in children under 5 years is observed more in winter. Key words: Yersinia enterocolitica, Diarrhea, children, Gorgan
M Naderinasab, N Tayyebi Meibodi, Y Nahidi, A Bakhshizadeh,
Volume 7, Issue 3 (Autumn 2013)
Abstract
Abstract
Background and Objective: Cross-transmission of microorganisms by the hands of health care workers is considered as a main transmission route of nosocomial infections. The aim of this study was to investigate the microbial contamination of health-care worker’s hands while going out of hospital.
Material and Methods: Wearing the sterile glove with liquid culture, we obtained 100 Samples from the staff’s hands of three departments (clerical department, emergency ward and central laboratory) of Emam Reza hospital. After that, the samples were cultured.
Results: Of all personnel, 40% have the habit of washing their hands. Of these, 95 percent wash their hands with water and soap, and 5 percent with alcohol rubs. Of 100 cultured samples, 90 have microorganisms including non-pathogen gram-positive bacillus (29%), coagulase-positive staphylococcus (39%), coagulase-negative staphylococcus (47%), Enterococci (3%), micrococcus (25%) and candida (3%). Contamination in those who had not washed their hands is 62.6% and in those who washed is 37.7% (P=0.04).
Conclusion: Hands of health-care workers become progressively contaminated by the potential pathogens during daily activities. To reduce the rate of contamination, it is helpful if we ask staff to wash their hands while going out of hospital.
Keywords: Microbial Flora Hospital’s Staff Hand Washing
Derakhshan, S, Najar Peerayeh, Sh, Fallah, F, Bakhshi, B,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Multiple drug resistance has increased in recent years in Klebsiella pneumoniae isolates. The Integrons are mobile genetic elements that carry antibiotics resistance genes. The aim of this study was to determine antibiotic susceptibility and the prevalence of class 1, 2, and 3 integrons in clinical Klebsiella pneumoniae isolated from clinical specimens. Material and Methods: A total of 108 K. pneumoniae isolates were collected between April and December 2011 from different clinical specimens of Loghman hospital in Tehran and identified by biochemical tests. Susceptibility of isolates to 14 antibiotic disks was determined by disk diffusion method. The template DNA was extracted by freeze-thaw method and the presence of class 1, 2, and 3 integrons was investigated by PCR method. Level of resistance to antibiotics in integron-positive and integron-negative isolates was determined. Results: The highest level of resistance was seen for cefotaxime, ceftriaxone, and amoxicillin-clavulanic acid (55.5%). In 79 isolates (73.14%) class 1 integron and in 57 of 79 isolates (72.15%) resistance to at least two classes of drugs were seen. The class 2 and 3 integrons were not detected. Among integron-negative isolates, 8 isolates (27.58%) had resistance to at least one antibiotic. Conclusion: The prevalence of class 1 integron in resistant K. pneumoniae is high therefore, the monitoring of drug resistance and limiting the use of antibiotics are necessary. Keywords: Klebsiella Pneumoniae, Integron, Multi-Drug Resistance
Parisa Bakhshi , Massoud Saidijam, Delavar Shahbazzadeh, Nazanin Mohajerani, Hassan Mirzahoseini,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract
Abstract
Background and Objective: Hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. Recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. The aim of this study was to evaluate the effect of inserting protein A signal peptide sequence of pEZZ18 plasmid on expression and secretion of the recombinant hirudin in E.coli.
Methods: the synthetic hirudin gene was amplified by PCR using specific primers. First, the gene was purified and cloned into PTG19-T cloning vector, and then it was subcloned into pEZZ18 expression vector by SalI / SacI enzymatic digestion and finally transformed into E.coli JM107. After the expression of recombinant hirudin protein, different cellular fractions were isolated and analyzed on SDS-PAGE and further confirmed by Western blotting.
Results: PCR product (522 bp) was first subcloned into the T-Vector (replicating vector) and then successfully subcloned into the pEZZ18 (expression vector). Cloning and subclonig were confirmed by enzymatic digestion and Colony PCR. After the expression and isolation of fractions, the presence of hirudin (about 29 kDa) in different cell fractions due to the effects of signal peptide was observed in SDS-PAGE and finally confirmed by Western blotting.
Conclusion: The gene of anticoagulant hirudin protein (desirudin) was cloned into the pEZZ18 vector containing Protein A signal peptide sequence and later transformed into E.coli JM107. The recombinant hirudin protein expression in the extracellular space was approved.
Keywords: Hirudin; Desirudin; Protein Sorting Signals.
Farzane Salarneia , Sare Zhand , Behnaz Khodabakhshi , Alijan Tabarraei , Mohammad Ali Vakili , Naeme Javid , Masoud Bazori , Abdolvahab Moradi ,
Volume 10, Issue 1 (Jan,Feb 2016 2016)
Abstract
Abstract
Background and objective: Hepatitis B virus (HBV) is a DNA virus with high tendency toward hepatic tissue. There are currently about 3 million HBV-infected people and 350 to 400 million chronic carriers of this virus in the world. X protein plays a role in the over-expression of oncogenes, carcinogenicity of liver cells and overlaps with the basal core promoter of the virus. Mutations at specific nucleotides of this region increase viral replication and liver disease progression. The aim of this study was to investigate the frequency of mutations at nucleotides 1762, 1764 and 1766 of HBV X gene in patients with chronic hepatitis B and hepatitis B-related cirrhosis.
Methods: In this study, 102 patients including 68 chronic hepatitis patients and 34 patients with hepatitis B-related cirrhosis were enrolled. After DNA extraction, HBV X gene was amplified and sequenced using Semi Nested-PCR. Obtained gene sequences were compared with the standard sequence of HBV virus X gene available in the gene bank (Okamoto AB033559). Then, the mutations in the gene X of HBV were identified.
Results: Comparison of the standard sequence with sequences obtained from patients showed the presence of A1762T / G1764A mutation in 12 chronic (17.64%) and 13 cirrhotic (38.23%) patients. Also, C1766G / G1764T mutations were found in 8.23% of chronic patients and 17.64% of cirrhotic patients.
Conclusion: A1762T / G1764A mutations in the overlapping region of the basal core promoter with gene X C-terminal may lead to liver disease progression from chronic hepatitis to cirrhosis, by changing the amino acid sequence of the X protein.
Shahram Zehtabian, Reza Alibakhshi, Seyed Yousef Seyedena, Ali-Reza Rai,
Volume 15, Issue 5 (Sep-Oct 2021)
Abstract
Background and objectives: Coronary artery disease (CAD) refers to stenosis or obstruction of coronary artery due to atherosclerosis or clotting. The aim of this study was to evaluate possible association of serum miRNA-197 (miR-197) expression as a biomarker for CAD diagnosis.
Methods: In this study, 100 patients with CAD who had angiography and vascular transplantation were selected. Expression of miR-197 was evaluated using real-time RT-PCR technique and the SYBR Green method. The Pearson's correlation coefficient was used to determine relationship of miR-197 expression and severity of coronary artery disease. The t-test was used to determine significance of expression of miR-197 in the study groups. All statistical analyses were carried out in SPSS 16 and at significance of 0.05.
Results: The results showed a direct relationship between miR-197 expression and CAD severity. The relative expression of miR-197 in the CAD patients was significantly higher than that in control subjects (P<0.004).
Conclusion: It seems that miR-197 can be considered as an indicator of coronary endothelial cell function. This microRNA could be used as a biomarker for CAD prognosis and treatment progression.
Ommolbanin Younesian, Behnaz Khodabakhshi, Sara Hosseinzadeh, Seyedeh Somayeh Hosseini Alarzi, Samareh Younesian, Mojtaba Pourmomen, Mana Zakeri, Ali Hosseini, Professor Hamidreza Joshaghani,
Volume 17, Issue 5 (Sep-Oct 2023)
Abstract
Background: Although public health interventions have slowed the spread of SARS CoV 2 infections, the worldwide pandemic of COVID 19 is progressing. Thus, effective and safe vaccination against SARS CoV 2 is an important tool for controlling the COVID 19 pandemic. Now in the early stages of COVID 19 vaccination, vaccinated individuals are interested in using antibody tests to confirm vaccination success and estimate the time of protection. Here, we assessed anti spike IgG responses in the general population 2 weeks after the second dose of the Sputnik V vaccine.
Methods: This study included blood samples of 67 individuals without a previous SARS CoV 2 infection taken 14 days after the second dose of the Sputnik V vaccine. Anti spike IgG responses were assessed with an enzyme linked immunosorbent assay (ELISA).
Results: Anti spike IgG was detected in 55 (82.1%) of 67 samples 14 days after the second dose of the Sputnik V vaccine. Antibody levels were significantly lower in males than in females, and 9 (75%) of 12 seronegative individuals were males.
Conclusion: Vaccination resulted in detectable anti spike IgG in 82.1% of individuals, and gender may be an important factor in the humoral response.
