Showing 16 results for Bagheri
H Bagheri, A Ghaemi, M Aslani, N Mozafari, S Livani, T Dadgar,
Volume 2, Issue 1 (Spring - Summer 2008[PERSIAN] 2008)
Abstract
Abstract Background and objectives: Diarrhea is one of the main cases of morbidity and mortality among children in developing countries. Enteroaggregative Escherichia coli (EAggEC) is an emerging diarrheal pathogen that has been associated characteristically with persistent diarrhea among infants, particularly in the developing Counties. Therefore, we decided to study the prevalence of enteroaggregative strain in cases of Diarrhea in Gorgan by PCR method. Material and Methods: This descriptive study was carried out on 455 subjects suffered from Diarrhea in Gorgan during one year (2005-6). At first, the samples were cultivated on the MacConkey agar and EMB agar media, Then all colony Suspected to E.coli were chosen and their DNA extracted by phenol chloroform method. The result was obtained by the selected primer, PCR method. Results: of 455 samples, Twenty cases (4/4%) including men (12) and woman (8) are positive for EAggEC, 85% of sufferers are under 5 years old (45.8% of them are under one year old). The Prevalence of this gene in Summer , Autumn ,Winter ,Spring are 5.3% , 4.2% , 4.1% and 1.8% ,respectively. Conclusion: Based on the prevalence of Enteroaggregative Escherichia coli (EAggEC) in Diarrheagenic cases in Gorgan (4.4%), we do recommend using molecular methods, which are reliable and less expensive than classic methods, in detecting of microorganisms. Key words: Entroaggregative Escherichia coli, Diarrhea, PCR, Gorgan.
Mirbagheri M, Taghipour H R, Farhadi N, Mirbagheri L, Imani Foladi Aa, Nourani M R,
Volume 6, Issue 1 (spring-summer[PERSIAN] 2012)
Abstract
Abstract Background and objectives: cardiac surgery is often associated with acute kidney injury (AKI). Nowadays, AKI is typically diagnosed by an increase in serum creatinine, which is a delayed and unreliable biomarker. Recent studies recommended using the liver type fatty acid binding protein (L-FABP) as an early biomarker. Material and Methods: The urine samples of 18 adult patients undergoing cardiac surgery were collected in different times before (2, 4,8,24 hour) and after cardiac surgery for detection of L-FABP by Elisa. Results: The results from ELISA test show that the increasing amount of L-FABP in urine samples of 4 patients is a diagnostic indicator for AKI. The mean concentration of L-FABP has increased up to 17 times at 8 hours after cardiac surgery compared to before surgery. Conclusion: according to our findings, we speculated that the urinary L-FABP can be a reliable and rapid biomarker for diagnosis of acute kidney injury. Key words: Acute Kidney Injury, Liver type Fatty Acid Binding Protein, Cardiac surgery
H Bagheri, F Najafi, N Behnampour, Ea Ghaemi,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract
Abstract
Background and objective: The periodic evaluation of antimicrobial activity of different antibiotic is essential because antibiotic sensitivity pattern may also changed during short courses. The aim of this study was to assess the frequency of Multi-drug Resistance (MDR) in Gram negative uropathogens.
Material and Methods: This study was conducted on 111 gram negative uropathogens using standard microbiology methods in Gorgan, 2011-2012. Antibiotic susceptibility was investigated by Kirby-Bauer disk diffusion methods (DDM).
Results: the most common isolates were klebsiella ( 40.5%) , Enterobacter (26.1%) , pseudomonas (13.5%) , proteus( 6.3%) , acinetobacter (1.8% ) and other gram negative bacteria ( 18.3%) .The highest antibiotic resistance was seen to clindamycin (99.1%), and the most sensitivity to Carbapenems (94.6%).Multi drug resistant was seen in 68.5% of isolates. In inpatients, all of the citrobacter species had resistant to multi drugs simultaneously.
Conclusion:a high frequency of multi drug resistant in uropathogens is observed in both inpatients and outpatients.
Keywords: Multi Drug Resistant, Gram Negative Bacteria, Urinary Tract Infection
Hosseini, Ma, Baniaghil, Ss, Mahmoodi, E, Bagheri Sani, Y, Dahbashi, Gh,
Volume 9, Issue 3 (Jul,Aug2015[PERSIAN] 2015)
Abstract
Abstract
Background and Objective: The donor selection and screening of blood donors for infectious factors is the main target of blood transfusion organization. this study aimed at determining the prevalence of HCV, HBV and HIV in blood donors of Golestan province , from 2012 to 2013.
Material and Methods: This cross-sectional study was conducted on all files of blood donors with positive lab results in Golestan Blood Transfusion Services. The test results, demographic characteristics and the Prevalence of hepatitis B and C and HIV were determined . The results were analyzed by SPSS software.
Results: The blood donors were 47,830 and 45,811 in 2012 and 2013, respectively. The Prevalence of HBV in 2012 was%0. 299 and in 2013 %0. 284; of HCV % 0. 06 in 2012 and % 0.05 in 2013, and the prevalence of both HCV and HIV totally was 0. 001percent in two years of study. The highest rate of positive case was among the first time donors, married and in those with less than high school education (P<0.05).
Conclusion: Regarding the high rate of positive cases among the first-time blood donors, married and less educated, it is recommended that public media be used for giving information to control communicable infections.
Keyword: HBV; HCV; HIV; Prevalence; Blood Donors .
Mahmoud Alebouyeh , Zahra Abedi , Hossein Rastegar , Hasan Bagheri , Javad Vaez, Behrouz Akbari-Adergani ,
Volume 9, Issue 5 (Nov,Dec-2015 2015)
Abstract
Abstract
Background and Objective: Aluminum salts are among the most common useful additive compounds in preparation of human and animal vaccines. Aluminum phosphate and aluminum hydroxide are two additives that show good immunoadjuvant effects with many antigens. Aluminum-containing vaccines lead to a better and longer immune response compared to adjuvant-lacking vaccines. The Chromogenic methods used for determination of aluminum amounts in manufacturing centers are time-consuming and requires some experienced technicians to obtain accurate results. This study aimed to design and validate a simple polarographic method to measure aluminum in recombinant hepatitis B vaccine.
Methods: In this study, the effects of temperature, pH, potential range and potential scan rate on the polarographic method of measuring aluminum in hepatitis B vaccine was evaluated and the optimal values for each of these factors were achieved.
Results: In order to measure aluminum, temperature of 60 °C and pH of 4.5 were found as the optimal values. Implementation of polarographic method in the potential range of -0.25 to 0.1 volts had a better signal.
Conclusion: Since the polarography method is more simple, accurate and faster than the chromogenic methods, it is suitable to be used for the measurement of aluminum in hepatitis B vaccine and it is recommended to be used in quality control laboratories for biological products.
Keywords: Adjuvant, Hepatitis B Vaccine, Polarography, Aluminum.
Seyedeh Masoumeh Mirtaghi, Parisa Torbati Nejad , Masoumeh Mazandarani, Fasiheh Livani, Hanieh Bagheri ,
Volume 10, Issue 5 (Sep-Oct-2016 2016)
Abstract
Background and Objective: Nowadays, incidence of antibiotic-resistance among pathogenic bacteria has increased due to indiscriminate use of antimicrobial drugs for treatment of diseases, especially urinary tract infections. Medicinal plants are also of great importance as antibacterial agents. Therefore, the aim of this study was to determine the antibacterial effect of ethanolic extract of nettle (Urtica dioica L.) leaves using two methods of disk diffusion and well diffusion.
Methods: Ethanolic extract of nettle leaves was prepared by the percolation method. Effect of different concentrations of the extract on Escherichia coli (PTCC1399), Staphylococcus aureus (PTCC 1431), Staphylococcus epidermidis (PTCC 1435) and Staphylococcus saprophyticus (PTCC1440) was evaluated using the disk diffusion and well diffusion methods by measuring diameter of growth inhibition zone. Gentamicin and propylene glycol were used as positive and negative control, respectively.
Results: In both methods, especially in the well diffusion, the ethanolic extract of nettle leaves had favorable inhibitory effect on the growth of S. aureus, S. epidermidis and S. saprophyticus. In the well diffusion method, the highest rate of susceptibility to the extract (89%) was related to S. saprophyticus and S. epidermidis.
Conclusion: The ethanolic extract of nettle leaf has good inhibitory effect on the growth of S. aureus (especially in the well diffusion method), which confirms the traditional use of this plant for the treatment of urinary tract infections.
Keywords: Antibacterial Effect, Staphylococcus, E. Coli, Ethanolic Extract, Nettle (Urtica dioica L.).
Vahide Vahideh Assadollahi , Masoume Jalalvand, Shahrokh Bagheri, Hamed Esmaiel Lashkarian ,
Volume 10, Issue 6 (Nov-Dec-2016 2016)
Abstract
ABSTRACT
Background and Objective: Multipotent placental amniotic membrane mesenchymal stem cells (MSCs) are capable of differentiating into specialized tissues under different conditions. The aim of this study was to induce differentiation of placental amniotic membrane MSCs from NMRI mouse into hepatocytes using liver extract.
Methods: Placental amniotic membrane MSCs from a 14-day pregnant female mouse was used in this study. The cells were incubated with trypsin solution, followed by pipetting. The resulting suspension was cultured in 12-well plates. After confirming their mesenchymal nature, differentiation of the aforementioned cells was induced via exposure to 6, 18, 30 and 60 μg/ml of liver extract. On the 16th day of treatment, immunocytochemical reaction for albumin and periodic acid-Schiff (PAS) test were performed for detection of hepatocyte-like cells.
Results: Change was observed in the shape of differentiating cells from spindle-like shape to polygonal shape. The immunocytochemical reaction of the differentiated cells was positive. PAS staining also confirmed the accumulation of glycogen particles in the aforementioned cells. Concentration of 6 μg/ml liver extract was found as the effective dose for induction of differentiation.
Conclusion: The findings of this study show that the placental amniotic membrane-derived MSCs of mouse can differentiate in vitro from spindle-like cells to polygonal hepatocyte-like cells with large nuclei and under the influence of the liver.
Keywords: Placental Amniotic Membrane Mesenchymal Stem Cells, Hepatocyte, In Vitro.
Oghlniaz Jorjani , Abdolrahman Bahlkeh , Faramarz Koohsar , Beniamin Talebi , Ali Bagheri ,
Volume 12, Issue 2 (Mar-Apr 2018)
Abstract
ABSTRACT
Background and Objective: Lophomonas blattarum is a multi-flagellate protozoan that is commensal in hindgut of cockroaches and other insects. The protozoan can cause respiratory infection in humans. Most cases of the infections with this protozoan have been reported in China. Here, we present a case with chronic respiratory allergy caused by
L. blattarum in Golestan province, Iran.
Case Description: The case was a 37-year-old male with history of respiratory conditions and he was immunocompetent. An athlete.
L. blattarum was detected in direct smear examination of sputum,
Conclusion: Since we found respiratory infection in an immunocompetent individual who was also an athlete, it is necessary to study this parasite and its life cycle and transmission methods. It is also suggested to consider
L. blattarum infection and treatment with metronidazole in cases of chronic allergies, especially those that do not respond to treatment.
Keywords: Lophomonas blattarum, Respiratory Allergies, Iran.
Behrouz Farhadihosseinabadi , Fahimeh Hosseini , Pegah Larki , Nader Bagheri , Kazem Abbaszadeh-Goudarzi , Koushan Sinehsepehr , Behrooz Johari , Meghdad Abdollahpour-Alitappeh ,
Volume 12, Issue 5 (Sep-Oct 2018)
Abstract
ABSTRACT
Breast cancer remains the most common cancer of women and one of the most common causes of cancer-related deaths worldwide. In spite of major advances in breast cancer diagnosis and treatment, the incidence of breast cancer remains high and the treatment of metastatic breast cancer remains challenging. This review presents an overview of breast cancer with a particular focus on its clinical aspects and therapies.
Keywords: Breast Cancer, Risk Factors, Diagnosis, Treatment.
Reza Bagheri, Amir Rashidlamir, Seyyed Reza Attarzadeh Hosseini,
Volume 12, Issue 6 (Nov - Dec 2018)
Abstract
ABSTRACT
Background and objectives: The present study was designed to determine the effect of blood flow restriction training (BFRT) on follistatin to myostatin ratio, body composition and anaerobic power of trained volleyball players.
Methods: Eighteen trained volleyball players were randomly assigned into two study groups: resistance training with blood flow restriction (BFRT; n=9) and resistance training without blood flow restriction (WBFRT; n=9). The subjects performed trainings three sessions a week, for eight weeks. In each session, barbell squat, leg extension, leg curl and dumbbell lunges were performed in three sets of 15 repetitions, with rest intervals of 30 seconds. Serum follistatin and myostatin concentrations, body composition and anaerobic power were assessed before and after the study. Data were analyzed using the paired sample t-test, Wilcoxon test, independent sample t-test and Mann-Whitney U test. All statistical analyses were done in SPSS (version 22), and a P-value of less than 0.05 was considered statistically significant.
Results: Follistatin levels increased significantly (P=0.001), while myostatin levels decreased significantly in both groups (P=0.001). Follistatin to myostatin ratio increased significantly in both groups (P=0.001). Although body fat percentage decreased in both groups, it did not differ significantly between the two groups (P=0.28). Moreover, anaerobic power increased significantly in both groups (P=0.001), but this increase was more profound in the BFRT group (P=0.001).
Conclusion: Based on our findings, blood flow restriction can be applied as remarkable approach to boost body adaptation responses to resistance training.
KEYWORDS: Resistance training, Myostatin, Follistatin, Blood flow restriction.
Farida Fazel , Artmis Naghibzadeh , Reza Mohammad Ramezanpour , Reza Bagheri , Azar Hamidi , Amir Rashidlamir,
Volume 13, Issue 3 (May-Jun 2019)
Abstract
ABSTRACT
Background and Objectives: Coronary heart disease has a direct correlation with plasma levels of Apolipoprotein B (ApoB) and low-density lipoprotein (LDL) and an inverse relationship with high-density lipoprotein (HDL) level. This study aimed at comparing effect of eight weeks of aerobic training with and without green coffee supplementation on serum ApoB level and atherogenic indices of overweight men.
Methods: Thirty overweight men were randomly divided into two groups: training + green coffee supplementation (T+G; n=15) and training + placebo (T+P; n=15). Participants in both groups performed aerobic training, three sessions per week for eight weeks. Initial exercise intensity was set at 50% of maximum heart rate but gradually increased to 75% of maximum heart rate in the last two weeks. In a single-blind design, the subjects in the T+G group received a 400 mg capsule of green coffee bean extract one hour before each exercise session. The T+P group received placebo at the same time. Paired sample t-test and independent t-test were used to compare intra-group and inter-group variations, respectively. All statistical analyses were performed using SPSS (version 22) at significance level of 0.05.
Results: ApoB, LDL/HDL and total cholesterol/HDL decreased significantly in both groups. However, the changes were more notable in the T+G group compared to the T+P group.
Conclusion: The eight-week training program along with green coffee supplementation has positive effects on serum ApoB and atherogenic indices of overweight, inactive men. Therefore, it can be suggested as a non-pharmacological method of preventing cardiovascular disease.
Keywords: Aerobic exercise, green coffee, Overweight, Apolipoprotein b.
Reza Bagheri, Samane Darroudi, S Mojtaba Hosseini, Hossein Nikkar, Fatemeh Khodadadi, Somayye Kasraee, Mahbube Sazegar, Amir Rashidlamir,
Volume 14, Issue 3 (May-Jun 2020)
Abstract
Background and objectives: Atherosclerotic cardiovascular disease is the leading cause of death in industrialized countries. High level of plasma lipids including cholesterol and triglycerides is one of the most important risk factors of atherosclerosis. Previous studies have shown that three members of the ATP-binding cassette, subfamily G (ABCG4, ABCG5 and ABCG8) are involved in transporting sterols across membranes. The purpose of this study was to assess the effect of high-intensity resistance training (HIRT) and aerobic exercise (AE) on expression of the ABCG4, ABCG5 and ABCG8 genes in female athletes.
Methods: Twenty-four female athletes from the Khorasan Province (Iran) were randomly selected and assigned into three groups: control (N=8), AE (N=8) and HIRT (N=8). The subjects in the HIRT group and the AE group performed exercise at intensity of 75-80% of 1-repetion maximum and 75-80% of maximum heart rate, respectively. Blood samples were collected at baseline and immediately after the exercise session. After isolation of lymphocytes by centrifugation and purification of mRNA, gene expression changes were investigated by Real-Time-PCR. Data were analyzed using one-way ANOVA and the Tukey's test.
Results: Both training protocols significantly increased the expression of ABCG4, ABCG5 and ABCG8 in the subjects (P<0.05), and there was no significant difference in the expression of these genes between the experimental groups (P>0.05).
Conclusion: A single session of HIRT and AE may have beneficial effects on prevention of atherosclerosis.
Ezzat Allah Ghaemi, Fahimeh Azadi, Naeme Javid, Hanieh Bagheri,
Volume 14, Issue 4 (Jul-Aug 2020)
Abstract
Background and objectives: Drug resistance in Staphylococcus aureus and Escherichia coli, as severe pathogenic bacteria, has become a health challenge. However, nanoparticles have been introduced as effective candidates for their eradication. In this study, we investigated presence of genes involved in conferring resistance to silver nanoparticles in S. aureus and E. coli isolates and evaluated its association with minimal inhibitory concentration (MIC) of the nanoparticles against these isolates.
Methods: The MIC of silver nanoparticles against 121 clinical isolates of E. coli and 183 S. aureus isolates was assessed by broth microdilution assay. Presence and expression of the silver resistance genes (silE, silR/S) in the isolates were investigated by PCR and real-time PCR, respectively.
Results: The silE gene was found in three (1.6%) S. aureus and four (3%) E. coli isolates. MIC of silver nanoparticles against S. aureus isolates with the silE gene was 1, 2 and 8 µg/ml. Moreover, the MIC of the nanoparticles against silE-positive E. coli isolates was 16 μg/ml in three cases and 8 μg/ml in one case. None of the S. aureus isolates contained the silR/S gene, but presence of both silE and silR/S was confirmed in two E. coli isolates. Real-time PCR showed no sil expression in the isolates containing the resistance genes.
Conclusion: The frequency of the silver resistance genes among S. aureus and E. coli isolates is very low. There is no relationship between presence of the resistance genes and the MIC value of silver nanoparticles.
Roghayeh Norouzi, Sajjad Jafari, Haniyeh Meshkati, Fahimeh Bagheri Amiri, Abolghasem Siyadatpanah,
Volume 15, Issue 1 (Jan-Feb 2021)
Abstract
Background and objectives: Head louse, also known as Pediculus humanus capitis, is an obligate ectoparasite that is a distributed all around the world. The aim of present study was to determine the prevalence of head lice infestation in primary school students in Tabriz, East Azerbaijan Province, Iran.
Methods: Overall, 3,032 students (1,515 boys and 1,517 girls) from 33 primary schools in Tabriz were selected via multi-stage cluster sampling in 2018. The students were examined individually and privately by experts. Head lice infestation was confirmed by detecting adult or nymph or nits less than 1 cm from base of hair. Data analysis was carried out in SPSS software package (version 23). Association of participants’ characteristics with pediculosis infestation was evaluated using logistic regression and chi-square test at significance level of 0.05.
Results: Head lice infestation was present in 130 (4.29%) students, while the prevalence of this condition was higher in girls (2.11%) than in boys. The highest prevalence (6.05 %) was observed in children age 6 years old regardless of gender. The rate of head lice infestation was lowest among students aged ≥12 years (3.17%). There was a significant relationship between head louse infestation and parent’s occupation, family size, the frequency of combing and hair style.
Conclusion: Although the prevalence of head lice infestation in Tabriz is relatively low, screening and treating schoolchildren should be done continuously in order to reduce the infestation rate.
Fahimeh Azadi, Masoomeh Rezanezhadi, Hanieh Bagheri, Laith B Alhusseini, Hamid Reza Joshaghani,
Volume 15, Issue 4 (Jul-Aug 2021)
Abstract
Background and objectives: Tuberculosis (TB) is a serious public health problem and a significant diagnostic and therapeutic challenge worldwide. Molecular diagnostic techniques are crucial parts of the World Health Organization’s new tuberculosis control strategy. This study aims to identify Mycobacterium tuberculosis and rifampin resistance in pulmonary and extra-pulmonary clinical specimens using the Gene Xpert MTB/RIF assay.
Methods: The study was carried out on 220 specimens from pulmonary and extra-pulmonary TB patients that were sent to the Kavosh Laboratory in Gorgan (Iran) during 2018-20. The Gene Xpert MTB / RIF method was applied to detect M. tuberculosis and rifampin resistance.
Results: Of 220 specimens, 15 (6.81%) were found to be positive, four (26.6%) of which were related to pulmonary and 11(73.3%) to extra-pulmonary specimens. None of the positive samples was resitant to rifampin according to assay.
Conclusion: Our findings demonstrate that the Gene Xpert MTB/RIF is able to accurately detect M. tuberculosis in pulmonary and extra-pulmonary specimens. The accurate and early diagnosis of TB infection allows timely therapeutic intervention, which is beneficial not only for the patient but also for possible contacts.
Fatemeh Bagherian, Alireza Nikoonejad, Abbas Allami, Samira Dodangeh, Layla Taha Yassen, Bahram Hosienbeigi,
Volume 15, Issue 6 (Special issue (Nov-Dec) 2021)
Abstract
Background and objectives: Antibiotic resistance is a global health challenge that affects both individuals and the health system in many ways. The aim of this study was to evaluate the antibiotic resistance pattern in isolates from patients admitted to the intensive care unit (ICU) of a hospital in Qazvin, Iran.
Methods: This descriptive and retrospective study was performed on urine and blood samples collected from 1318 ICU patients in the Velayat Hospital of Qazvin (Iran) during 2017-2019. Data were collected from patients’ medical records. All statistical analyses were performed using SPSS software (version 25).
Results: Based on the findings, 65.2% of the samples were related to urinary tract infections and 34.7% to bloodstream infections. Escherichia coli (68.6%) and Stenotrophomonas (41.0%) were the most common bacteria isolated from urinary tract infections and bloodstream infections, respectively. Moreover, the rate of antibiotic resistance was higher among Acinetobacter, Escherichia coli, Stenotrophomonas, Enterococcus and Pseudomonas isolates.
Conclusion: The rate of drug resistance in isolates from ICU patients is alarmingly high and requires immediate attention. It is recommended to modify antibiotic prescriptions in the hospital based on the results of antibiotic resistance pattern, particularly for treatment of infections caused by E. coli and Stenotrophomonas.
