Saadat, S, Solhjoo, K, Norouz-Nejad, Mj, Kazemi, A, Erfanian, S, Ashrafian, F,
Volume 8, Issue 4 (supplement Issue[PERSIAN] 2015)
Abstract
Abstract Background and Objective: Vancomycin is used for treatment of methicillin-resistant S. Aureus (MRSA) infections therefore, resistance to this antibiotic is increasing. We aimed to determine the antibiotic resistance pattern and frequency of vancomycin resistant S. Areas (VRSA) strains isolated from clinical samples. Material and Methods: In this cross-sectional study, 100 S. Aureus isolates collected from hospitals in Shiraz during six months, 2012, were identified by biochemical, microbiological and molecular methods. After determination of antibiotic susceptibility pattern by disc diffusion method and vancomycin agar screening test, Minimum Inhibitory Concentration (MIC) was determined by E-test for vancomycin, thicoplanin, linezolid and quinupristin-dalfopristin. Results: The most resistant and the most sensitive antibiotic were ampicillin (%95) and quinupristin-dalfopristin (99%), respectively, and 44% of isolates were resistant to methicillin. In agar screening test, 48% of strains had reduced sensitivity and in disc diffusion 3% strains were resistant to vancomycin. In E-test method, only one isolate was resistant to vancomycin. Conclusion: given the presence of VRSA and new antibiotic resistant strains, we recommend doing some intervention to prevent from spreading these strains in hospitals. . Keywords: Clinical Specimens, Staphylococcus Aureus, Vancomycin, Antibiotic Resistance
Mishar Kelishadi , Mandana Kelishadi , Akramsadat Ahmadi , Naeme Javid , G.hossein Ashrafi , Alijan Tabarraei ,
Volume 13, Issue 2 (Mar-Apr 2019)
Abstract
ABSTRACT
Background and objectives: Pterygium is a non-cancerous growth of conjunctival tissue that can extend onto the corneal surface. The presence of some oncogenic viruses in pterygium and the neoplastic nature of these lesions led us to the postulated involvement of the viruses in the etiology of pterygium. Given the
association of human herpesvirus 6 (HHV-6) with ocular diseases, we aimed to investigate
presence of this virus in
pterygium.
Methods: Fifty tissue specimens were collected from patients with pterygium who underwent pterygium surgery between February 2013 and May 2015. The specimens were tested by real-time PCR using
Maxima SYBR Green/ROX qPCR Master Mix (2X) kit. Demographic and clinical data were collected and analyzed using SPSS software (version 18).
Results: Six (12 %) specimens were positive for HHV-6 DNA. There was no statistically significant correlation between pterygium and presence of HHV-6.
Conclusion: Based on the results, a direct association between HHV-6 and development of pterygium seems less probable, which suggests that other etiologic agents must be involved in the multistep process of the disease.
Keywords: Human Herpesvirus 6; pterygium; Real-time PCR.
Mishar Kelishadi , Mandana Kelishadi , G.hossein Ashrafi , Alijan Tabarraei ,
Volume 13, Issue 4 (Jul-Aug 2019)
Abstract
ABSTRACT
Background and Objectives: Pterygium is a common ocular surface lesion that manifest as wing-shaped, benign
conjunctival growth, which can extend onto the corneal surface. Presence of some oncogenic viruses in pterygium and the neoplastic nature of the lesion led us to the postulated involvement of the viruses in the etiology of pterygia. The aim of this study was to evaluate prevalence and possible role of human cytomegalovirus (HCMV) in the formation of pterygia.
Methods: Fifty pterygium specimens and 10 normal conjunctival biopsy specimens (controls) were investigated by polymerase chain reaction using primers specific for the highly conserved regions of major capsid protein gene of HCMV. Data were analyzed using SPSS statistical software (IBM SPSS Statistics 18; IBM Corporation, USA) at significance level of 0.05.
Results: The HCMV DNA was detected in seven (14%) patients with pterygium but in none of the control subjects. All subjects were β-globin positive.
Conclusion: Given the results, direct involvement of HCMV in the development of pterygium seems less probable, thus suggesting that other agents might be involved in the multistep process of the disease.
Keywords: Human Cytomegalovirus, Pterygium, Polymerase Chain Reaction.
Mishar Kelishadi, Pezhman Hashemi, G.hossein Ashrafi , Naser Behnampour, Alijan Tabarraei,
Volume 13, Issue 5 (Sep-Oct 2019)
Abstract
ABSTRACT
Background and Objectives: Red blood cell (RBC) transfusion is necessary for the prevention and treatment of a variety of life-threatening injuries and diseases. However, viral contamination of these products is a great threat to recipients. Screening donors for GB virus C by nucleic acid testing is not routinely implemented worldwide. The aim of the present study was to evaluate prevalence of GBV-C RNA in whole blood/red cell components.
Methods: In this cross sectional pilot study, we collected 153 units of packed RBCs from blood banks of two public hospitals in Gorgan (northeast of Iran), between October and November 2014. The samples were screened for the presence of GBV-C RNA in plasma by nested RT-PCR using specific primers targeting highly conserved regions of 5' UTR of GBV-C. Data were analyzed using SPSS software (version 18).
Results: Overall, 48 (31.37%) whole blood or red cell components were positive for GBV-C viremia. The GBV-C RNA was detected in 31/88 citrate phosphate dextrose-adenine 1 (CPDA1) RBC, 16/50 washed RBC and 1/13 reduced-leukocyte RBC. However, whole blood CPDA1 was negative for GBV-C viremia. Direct sequencing of PCR products confirmed GBV-C contamination.
Conclusions: Transmission of GBV-C infection was observed in blood products. Thus, efforts should be made to develop new strategies for assuring blood transfusion safety.
Keywords: Molecular testing, Epidemiology, Transfusion-transmissible infections, GB Virus C.
