This paper should be cited as: Naghsh,N. Doudi,m.
The Antifungal Activity of Silver
Nanoparticles and Fluconazole on AspergillusFumigatus
Naghsh,N.(PhD)1, Doudi,m.(PhD) 2, Safaeinejad,Z.(MSc)*3
1.
Assistant Professor of Biology, Islamic Azad University, Falavarjan
Branch, Iran.
2. Assistant professor of Microbiology, Islamic Azad
University, Falavarjan Branch Iran.
3. MSc Student of Microiology,Islamic Azad University, Falavarjan
Branch, Isfahan, Iran
Abstract
Background
and objective: In
recent years, the resistance of opportunistic fungal strains to commercially available
antifungal agents has been increased. The serious side effects of these
compounds on mammalian cells forced the
researchers to search for new antifungal substances. Thus we decided to
investigate the antifungal properties of silver nanoparticles against Aspergillusfumigatus.
Materials & Methods:To investigate the antifungal effect
of the round silver nanoparticles with 10nm diameter against Aspergillusfumigatus, the diameter of colonies after
8 days as well as the number of colonies in different days was determined,
using direct drop test. After that, to measure the Minimum
Inhibitory Concentration (MIC) and Minimum Fungicide Concentration (MFC) values
of silver nanoparticles and fluconazole, Micro Dilution Broth method was
performed. At the end, the MIC and MFC values of silver nanoparticles were
compared to MIC and MFC of fluconazole.
Results:The results
obtained from direct drop test confirm that the silver nanoparticles
can decrease the diameter of fungal colonies in dose dependent manner. The data
of silver nanoparticles on the number of colonies in different days shows that
the number of colonies increases up to sixth day and then becomes
fixed. Based on the results of Micro
Dilution Broth method, the MIC and the MFC values of this component are 31.25
and 62.5ppm, respectively.
Conclusion:The present study confirms that silver
nanoparticles with 10nm diameter have antifungal activity against Aspergillus fumigates.
Key
words: Silver nanoparticles, Aspergillusfumigatus, Antifungal activity.
Corresponding author: Safaeinejad,Z
Email:safayinejad@yahoo.com
Received 21 Jan 2012 Revised23 Jan 2013 Accepted 9 Mar 2013