This paper should be cited as: Ghelich, T. Hashemi Karouei, M. Gholampor Azizi, I.

Ghelich, T. (MSc)1, Hashemi Karouei, M. (PhD)2, Gholampor Azizi, I. (PhD)3

1. MSc of Microbiology, Islamic Azad University, Tonekabon Branch, Iran

2. Assistant Professor of Mycology,Department of Microbiology, Islamic Azad University,Tonekabon, Iran

3. Assistant Professor of Mycology,Department of Biology, Islamic Azad University,Tonekabon, Iran

Antibacterial Effect of Methanolic Extraction of Polygonum Bistorta on Some Bacteria

Abstract

Background and Objective:Because of increased resistance to antibiotics, side effects of chemical drugs and importance of medicinal plants, we aimed to assess the antibacterial effects of methanolic extract of the Polygonumbistorta plant on the E. coli (ATCC 15224), Ps. aeruginosa (ATCC 25619), B. subtilis (ATCC 6633) and Stap. Aureus (ATCC 25923).

Material and Methods: After preparing the extract, its antibacterial effect was assessed via gel diffusion method, using disk/well diffusion methods to determine MIC and MBC.

Results:MIC of methanolic extract was 78µg/ml for E.coli,63×10³µg/ml for Pseudomonas aeruginosa,39µg/ml for Bacillus subtilis and31×10² µg/ml for Staphylococcus aureus.

Conclusion:In spite of resistance of gram-negative bacteria to chemical agents, polygonum bistorta methanolic extract could inhibit the growth of E.coli and P. aeruginosa.

Key words: Antibacterial, Bistorta, Escherichia Coli, Pseudomonas Aeruginosa

Corresponding Author:Hashemi Karouei, M.

Email: mssepid4977@gmail.com

Received 14 Jan 2014 Revised 1 Mar 2014 Accepted 4 Mar 2014