fa تحقيقي Original Paper <div><strong>ABSTRACT</strong><br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Background and Objective</strong>: MiRNAs are small RNAs that are expressed in most eukaryotes, and can regulate gene expression by attaching to the 3&rsquo; end of target mRNA. MicroRNA-101 (miR-101) post-transcriptional regulation is important for host-virus interactions. In addition, miR-101 has a tumor suppressive role in liver cancer and metastasis, and induces apoptosis in tumor cells. We examined miR-101 expression in patients with chronic hepatitis B, hepatitis B virus (<a href="http://www.scipers.com/ePaper-View-1991787=%D8%B1%DA%AF%D8%B1%D8%B3%DB%8C%D9%88%D9%86-%D8%AE%D9%88%D8%AF-%D8%A8%D9%87-%D8%AE%D9%88%D8%AF-%D8%A7%D8%B2-%D9%87%D9%BE%D8%A7%D8%AA%DB%8C%D8%AA-B-%D8%A8%D9%87-%D8%B3%DB%8C%D8%B1%D9%88%D8%B2.html">HBV)-associated cirrhosis</a> and healthy individuals.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Methods</strong>: The study was performed on 108 whole blood samples (36 samples from each group) collected in EDTA tubes. RNA was extraction by RNX-plus kit according to the manufacturer&rsquo;s protocol. Finally, miRNA expression was evaluated using relative real time PCR.<br> <strong>&nbsp;&nbsp; &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Results</strong>: A 2.4-fold increase was observed in miR-101 expression in patients with chronic hepatitis B, while there was a 3.5-fold increase in miR-101 expression in patients with <a href="http://www.scipers.com/ePaper-View-1991787=%D8%B1%DA%AF%D8%B1%D8%B3%DB%8C%D9%88%D9%86-%D8%AE%D9%88%D8%AF-%D8%A8%D9%87-%D8%AE%D9%88%D8%AF-%D8%A7%D8%B2-%D9%87%D9%BE%D8%A7%D8%AA%DB%8C%D8%AA-B-%D8%A8%D9%87-%D8%B3%DB%8C%D8%B1%D9%88%D8%B2.html">HBV-associated cirrhosis</a> compared with healthy controls (P=0.003). MiR-101 overexpression in patients with <a href="http://www.scipers.com/ePaper-View-1991787=%D8%B1%DA%AF%D8%B1%D8%B3%DB%8C%D9%88%D9%86-%D8%AE%D9%88%D8%AF-%D8%A8%D9%87-%D8%AE%D9%88%D8%AF-%D8%A7%D8%B2-%D9%87%D9%BE%D8%A7%D8%AA%DB%8C%D8%AA-B-%D8%A8%D9%87-%D8%B3%DB%8C%D8%B1%D9%88%D8%B2.html">HBV-associated cirrhosis</a> was more notable that in patients with chronic hepatitis B.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Conclusion</strong>: According to the results, evaluating miR-101 expression may predict disease progression from chronic hepatitis B to HBV-associated cirrhosis.<br> <strong>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; Keywords</strong>: <a href="https://www.ncbi.nlm.nih.gov/mesh/68035683">MicroRNAs</a>, Chronic Hepatitis B, Liver Cirrhosis, MiR-101.</div> MicroRNAs, Chronic Hepatitis B, Liver Cirrhosis, MiR-101. 10 14 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-456&slc_lang=fa&sid=1 Mahshid Zandi 100319475328460012543 100319475328460012543 No Department of Biology, College of Sciences, Damghan branch, Islamic Azad University, Damghan, Iran Mohammad Ebrahimifard 100319475328460012544 100319475328460012544 No Department of Biology, College of Sciences, Damghan branch, Islamic Azad University, Damghan, Iran Abdolvahab moradi abmoradi@gmail.com 100319475328460012545 100319475328460012545 Yes Departement of Microbiology. Golestan University of Medical Sciences, Gorgan, Iran