fa شناسایی ژن fli C(فلاژلین) در سودوموناس ائروژینوزا های جدا شده از نمونه های بالینی با استفاده از واکنش زنجیره ای پلی مراز(PCR) Abstract Background and objectives: Pseudomonas aeruginosa as an opportunistic pathogen can establish lethal infections in immunocompromised patients or those exposed to predisposing factors. This bacterium contains a single polar flagellum causing motility, chemotaxis and colonization in acute phase of infection. The flagella filament is made up of a structural protein called flagellin. This study was aimed at determining The frequency of fliC gene in Clinical Samples. Material and Methods: In this study, a pair of specific primer for types of flagellin (a, b type) was designed and by using PCR method its structural gene (fliC) was recognized and amplified in clinical strains. Results: This original primer has appropriate efficiency in diagnostic of pseudomonas aeroginosa flagellum. Our study shows that 85% of the Clinical Samples have a fliC gene. Conclusion: This method can be applied to recognizing of the motile strains, and their antigenic typing, and complete amplification of fliC sequence in order to cloning and expression of recombinant flagellin. Key words: Pseudomonas aeruginosa, flagellin, fliC, PCR Key words: Pseudomonas aeruginosa, flagellin, fliC, PCR 0 0 http://mlj.goums.ac.ir/browse.php?a_code=A-10-1-21&slc_lang=fa&sid=1 M Sattari مرتضی ستاری Sattarim@modares.ac.ir 100319475328460013194 100319475328460013194 Yes دانشکده علوم پزشکی،دانشگاه تربیت مدرس AA Imani fooladi عباسعلی ایمانی فولادی 100319475328460013195 100319475328460013195 No مرکز تحقیقات بیولوژی مولکولی دانشگاه« عج »… علوم پزشکی بقیه ا GH Godarzi غلامرضا گودرزی 100319475328460013196 100319475328460013196 No گروه باکتری شناسی، دانشکده علوم پزشکی، دانشگاه تربیت مدرس