en بیوشیمی Biochemistry تحقيقي Original Paper <strong>Background and objectives: </strong>Docetaxel is a chemotherapeutic agent commonly used for treatment of many cancers, including esophageal squamous cell carcinoma. Docetaxel induces G2/M phase cell cycle arrest and ultimately cell death. In this study, we aimed to assess the effects of docetaxel on YM1 cells considering exposure time and dose.<br> <strong>Methods:</strong> After calculating the doubling time of YM1 cells, the anti-proliferative effect of different concentrations of docetaxel <a>() </a><a href="#_msocom_1" id="_anchor_1" name="_msoanchor_1" uage="JavaScript">[A1]</a>&nbsp; after 24, 48 and 72 hours was assessed by the standard colorimetric assay. In addition, the effect of docetaxel on cell cycle was evaluated by flow cytometry.<br> <strong>Results:</strong> The results showed that docetaxel toxicity was not significant until 24 hours at the tested concentrations (P>0.05). In addition, the effect of docetaxel on the cells was time-dependent at all tested concentrations. Overall, the duration of exposure to docetaxel had more significant role in docetaxel toxicity in YM1 cells compared to concentration.<br> <strong>Conclusion:</strong> Our findings suggest that the cytotoxicity of docetaxel on YM1 cells is time-dependent. <div> <hr align="left" size="1" width="33%" > <div> <div id="_com_1" uage="JavaScript"><a name="_msocom_1"></a>&nbsp;<a href="#_msoanchor_1">[A1]</a>Please write the concentrations</div> </div> </div> Docetaxel,Cell cycle,Esophageal Neoplasms, 23 27 http://mlj.goums.ac.ir/browse.php?a_code=A-10-868-2&slc_lang=en&sid=1 Boshra haghi haghi.b@goums.ac.ir 100319475328460019113 100319475328460019113 Yes Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran Marie Saghaeian jazi marie.saghaeian@yahoo.com 100319475328460019114 100319475328460019114 No Stem cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran Mahdi zarie 100319475328460019115 100319475328460019115 No Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran Ayyoob Khosravi 100319475328460019116 100319475328460019116 No Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran mahboubeh tajaldini mtajodini@gmail.com 100319475328460019117 100319475328460019117 No Ischemic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran Jahanbakhsh Asadi dr.asadi@goums.ac.ir 100319475328460019118 100319475328460019118 No Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran