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mljgoums 2017, 11(4): 30-33 |
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Dashti M, Bahrami A, Sadeghian M H, Shams S F, Ashjaee A, Arianpour Z. Cells O Antibody Screening Cells: A novel Approach to Antibody Screening. mljgoums 2017; 11 (4) :30-33
URL: http://mlj.goums.ac.ir/article-1-987-en.html
URL: http://mlj.goums.ac.ir/article-1-987-en.html
Mohsen Dashti1 
, Afsane Bahrami2 , Mohammad Hadi Sadeghian3 , Seyyede Fatemeh Shams 4, Ahmad Ashjaee1 , Zahra Arianpour5
, Afsane Bahrami2 , Mohammad Hadi Sadeghian3 , Seyyede Fatemeh Shams 4, Ahmad Ashjaee1 , Zahra Arianpour5
1- Blood Banking Laboratory of Ghaem Hospital, Mashhad University of Medical Sciences (MUMS), Mashhad, Iran
2- Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3- Department of Hematology and Blood Banking, Mashhad University of Medical Sciences, Mashhad, Iran
4- Hematology Researcher, Mashhad University of Medical Sciences, Mashhad, Iran ,shams8869@yahoo.com
5- Iranian Blood Transfusion Organization Research Center, Mashhad, Iran
2- Department of Modern Sciences and Technologies, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
3- Department of Hematology and Blood Banking, Mashhad University of Medical Sciences, Mashhad, Iran
4- Hematology Researcher, Mashhad University of Medical Sciences, Mashhad, Iran ,
5- Iranian Blood Transfusion Organization Research Center, Mashhad, Iran
Abstract: (7753 Views)
ABSTRACT
Background and Objectives: Blood transfusion may induce some adverse effects on receivers. Some methods such as antibody screening and cross matching have been suggested to reduce the risk of transfusion complications. However, these methods require commercial antibody screening kits that may also need special equipment. The aim of this study was to introduce a new method for antibody screening that does not require a commercial kit, and could be used in any transfusion laboratory.
Methods: We examined 350 samples that contained alloantibody and 350 control samples without the antibody. A solution containing two O+ and one O- samples were used instead of screening cells.
Results: Sensitivity and specificity of the method were 73.32% and 45.15%, respectively. Positive predictive value and negative predictive value were 58.33% and 63.88%, respectively.
Conclusion: Our new method can be used in basic hematology laboratories with some modifications.
Keywords: Antibodies, Antigens, Coombs test.
Background and Objectives: Blood transfusion may induce some adverse effects on receivers. Some methods such as antibody screening and cross matching have been suggested to reduce the risk of transfusion complications. However, these methods require commercial antibody screening kits that may also need special equipment. The aim of this study was to introduce a new method for antibody screening that does not require a commercial kit, and could be used in any transfusion laboratory.
Methods: We examined 350 samples that contained alloantibody and 350 control samples without the antibody. A solution containing two O+ and one O- samples were used instead of screening cells.
Results: Sensitivity and specificity of the method were 73.32% and 45.15%, respectively. Positive predictive value and negative predictive value were 58.33% and 63.88%, respectively.
Conclusion: Our new method can be used in basic hematology laboratories with some modifications.
Keywords: Antibodies, Antigens, Coombs test.
Research Article: Original Paper |
Received: 2017/08/8 | Accepted: 2017/08/8 | Published: 2017/08/8 | ePublished: 2017/08/8
Received: 2017/08/8 | Accepted: 2017/08/8 | Published: 2017/08/8 | ePublished: 2017/08/8
References
1. Bain BJ, Win N. Dacie and Lewis Practical Haematology. 11th ed. 2012; 529-30.
2. Nicholls MD. Transfusion: morbidity and mortality. Anaesth Intensive Care. 1993; 21(1): 15-9.
3. Azarkeivan A AS, Ahmadi MH, Hajibeigy B, Maghsudlu M, Nasizadeh S, et al. Blood transfusion and alloimmunization in patients with thalassemia: multicenter study. Pediatric Hematology-Oncology. 2011; 28(6): 479-85. doi: 10.3109/08880018.2011.568595. [DOI:10.3109/08880018.2011.568595]
4. Yuan S EN, Bailey D, Salvador M, Wang S. Transfusion of multiple units of Js (b+) red blood cells in the presence of anti-Jsb in a patient with sickle beta-thalassemia disease and a review of the literature. Immunohematology/American Red Cross. 2007; 23(2): 75-80.
5. Carson JL GB, Kleinman S, Tinmouth AT, Marques MB, Fung MK, et al. Red blood cell transfusion: a clinical practice guideline from the AABB.Clinical TransfusionMedicine Committee of the AABB. Ann Intern Med. 2012; 157(1): 49-58. doi: 10.7326/0003-4819-157-1-201206190-00429. [DOI:10.7326/0003-4819-157-1-201206190-00429]
6. Lewis SSM BB, Bates I, Dacie JV. Dacie and Lewis's practical haematology. 10th ed. 2006.
7. Eder AF CL. Noninfectious complications of blood transfusion. Arch Pathol Lab Med. 2007; 131(5): 708-18.
8. Hendrickson JE HC. Noninfectious serious hazards of transfusion. Anesth Analg. 2009; 108(3): 759-69. doi: 10.1213/ane.0b013e3181930a6e. [DOI:10.1213/ane.0b013e3181930a6e]
9. McPherson RA, Pincus MR. Henry's clinical diagnosis and management by laboratory methods. 22th ed: Elsevier Health Sciences. 2011; 680.
10. GL. M. L evoluzione tecnologica in immunoematologia eritrocitaria. The Ligand Quarterly. 1993;12:466-74.
11. Keramati MR, Shakibaei H, Kheiyyami MI, Ayatollahi H, Badiei Z, Samavati M,
12. Hoffbrand AV, Catovsky D, Tuddenham EG. Postgraduate haematology. 6th ed: John Wiley & Sons. 2008; 238-9.
13. Weisbach V, Kohnhäuser T, Zimmermann R, Ringwald J, Strasser E, Zingsem J, et al. Comparison of the performance of microtube column systems and solid-phase systems and the tube low-ionic-strength solution additive indirect antiglobulin test in the detection of red cell alloantibodies. Transfusion Medicine. 2006; 16(4): 276-84. [DOI:10.1111/j.1365-3148.2006.00674.x]
14. Garozzo G, Licitra V, Criscione R, Comitini N, Noto C, Lomagno R, et al. A comparison of two automated methods for the detection and identification of red blood cell alloantibodies. Blood Transfusion. 2007; 5(1): 33. doi: 10.2450/2007.0022-06.
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