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Volume 10, Issue 6 (Nov-Dec-2016 2016)
mljgoums 2016, 10(6): 54-59 |
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Anzabi Y, Khaki A. Antibacterial Activity of Extract and Essential Oil of Ziziphora tenuior L. against Bacteria Isolated from Urogenital Tract Infections. mljgoums 2016; 10 (6) :54-59
URL: http://mlj.goums.ac.ir/article-1-917-en.html
URL: http://mlj.goums.ac.ir/article-1-917-en.html
1- Department of Pathobiology, Tabriz Branch
2- Department of Pathobiology, Tabriz Branch ,khaki@iaut.ac.ir
2- Department of Pathobiology, Tabriz Branch ,
Abstract: (15267 Views)
ABSTRACT
Background and Objectives: Many species of Ziziphora have been used in traditional medicine in the Azarbaijan region, northwest of Iran. This study aimed to determine antibacterial activity of essential oil and methanol extract of Ziziphora tenuior on some pathogenic bacteria isolated from urogenital tract infections.
Methods: The essential oil and methanol extract of Z. tenuior were obtained by Clevenger and maceration methods. Under sterile conditions, the required amount of urine was taken from patients referred to a number of clinics in Tabriz during 2014. After identification of the isolates using standard microbiological methods, antimicrobial effects of the essential oil and methanol extract of the plant on the isolates were evaluated by determining minimum inhibitory concentration (MIC), minimum bactericidal concentration, and antibiogram test.
Results: The MIC for essential oil of Z. tenuior was 250µg/mL for most Gram-negative bacteria except Pseudomonas aeruginosa. Moreover, the MIC for Staphylococcus aureus was 250µg/mL and 500µg/mL for other strains of Staphylococcus.
Conclusion: Comparison of the inhibitory and bactericidal effects of the essential oil and methanol extract of Z. tenuior showed that the essential oil is able to inhibit growth of the bacteria tested even in low concentrations. Further studies are required in this regard using animal models.
Keywords: Antibacterial Agents, Ziziphora tenuior, Plant Extracts, Urinary Tract Infection.
Research Article: Original Paper |
Received: 2016/01/25 | Accepted: 2016/01/25 | Published: 2016/09/25 | ePublished: 2016/09/25
Received: 2016/01/25 | Accepted: 2016/01/25 | Published: 2016/09/25 | ePublished: 2016/09/25
References
1. Thuille N, Fille M, Nagl M. Bactericidal activity of herbal extracts. Int J Hyg Environ Health. 2003; 206(3): 217-21. [DOI:10.1078/1438-4639-00217]
2. Baser K, Sezik E, Tumen G. Composition of the essential oil of Ziziphora clinopodioides Lam. J Essential Oil Res. 1991; 3(4): 237-239. [DOI:10.1080/10412905.1991.9697934]
3. Sardashti A. R, Valizadeh J, Adhami Y. Chemical composition of the essential oil from Ziziphora clinopodioids Lam. from Iran by means of gaschromatography-mass spectrometry (GC-MS). J Hortic For. 2012; 4(10): 169-171. DOI: 10.5897/JHF11.039.
4. Sajadi SE, Ghasemi Dehkordi N, Baloochi M. Volatile Constituents of Ziziphora clinopodioides Lam. Journal of research and reconstruction. 2003; 16(1): 97-100.
5. Anzabi Y. Evaluation of Antibacterial Activity of Aqueous Extracts of Onion and some Antibiotics on a Number of some Important Bacteria in Terms of Food Hygiene. Crescent Journal of Medical and Biological Sciences. 2014; 1(4): 136-142.
6. Khosrokhavar R, Ahmadiani A, Shamsa F. Antihistaminic and Anticholinergic Activity of Methanolic Extract of Barberry Fruit (Berberis vulgaris) in the Guinea- Pig Ileum.J.Med.Plants. 2010; 9(35): 99-105.
7. Shariff ZU. Modern herbal therapy for common ailments. Nature pharmacy series. Spectrum Books limited. United Kingdom. 2001; 9-84.
8. Salehi P, Sonboli A, Eftekhar F, Nejad-Ebrahimi S, Yousefzadi M. Essential oil composition, antibacterial and antioxidant activity of the oil and various extracts of Ziziphora clinopodioides subsp. rigida (BOISS.) RECH F. from Iran. Biol Pharm Bull. 2005; 28(10): 1892-1896. [DOI:10.1248/bpb.28.1892]
9. Naderinasab M, Rashed T, Nazem M. Laboratory bacteriology. Emam Reza Press.1997; 24-29.
10. Babayi L, Kolo JI, Ijah UJ. The antimicrobial activities of methanolic extracts of Eucalyptus camaldulensis and Terminalia catappa against some pathogenic microorganisms. Biochemistry. 2004; 16(2): 106-110.
11. Inouye Sh, Takizawa T, Yamaguchi H. Antibacterial activity of essential oils and their major constituents against respiratory tract pathogens by gaseous contact. J Antimicrob Chemother. 2001; 47(5): 565-573. doi: 10.1093/jac/47.5.565. [DOI:10.1093/jac/47.5.565]
12. Quinn PJ, Carter B, Markey ME, Carter GR. Clinical Veterinary Microbiology. Mosby Press. London, England. 1994; 95-102.
13. Rezai M, Rasooli I. Chemical components and biological activity of essential oils of Thymusxprolock and Mentha longifolia. J Daneshvar. 2000; 8(31): 1-8.
14. Sindambiwe JB, Calomme M, Cos P, Totte J, Pieters L, Vlietinck A, Vanden Berghe D. Screening of seven selected Rwandan medicinal plants for antimicrobial and antiviral activites. J Ethnopharmacol. 1999; 65(1): 71-7. [DOI:10.1016/S0378-8741(98)00154-8]
15. Nostro A, Germano MP, Angelo VA, Marino A, Connatelli MA. Extraction methods and bioautography for evaluation of medicinal plant antimicrobial activity. Lett Appl Microbiol. 2000; 30(5): 389-394. [DOI:10.1046/j.1472-765x.2000.00731.x]
16. Ali MS, Saleem M, Akhtar F, Jahangir M, Parvez M, Ahmad VU. Three p-cymene erivatives from Zararia multiflora. Phytochemistry. 1999; 52(4): 685-688. [DOI:10.1016/S0031-9422(99)00259-9]
17. Mazandarani M, Osia N, Ghafourian M. Antioxidant Activity and Ethno pharmacological Survey of Achillea biebersteinii Afan. in the Treatment of Dysmenorrhoea in Traditional Medicine of Golestan Province, Iran. International Journal of Women's Health and Reproduction Sciences. 2015; 3(2):107-110. [DOI:10.15296/ijwhr.2015.21]
18. Schaechter M, Medoff G, Fchlessinger D. Mechanisms of Microbial Diseases. International Edition, Williams and Wilkins. 1989; 17-50.
19. Nazer A, Kobilinsky A, Tholozan J, Dubais-Brissonet F. Combination of foods antimicrobials at low levels to inhibit the growth of Salmonella sv. typhimurium: a synergistic effect? Food Microbiol. 2005; 22(5): 391-398. [DOI:10.1016/j.fm.2004.10.003]
20. Sokmen A, Gulluce M, Akpulat HA. The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius. Food Control. 2004; 15(8): 627-634. [DOI:10.1016/j.foodcont.2003.10.005]
21. Tassou C, Koutsomanis K, Nychas GJE. Inhibition of Salmonella entritidis and Staphylococcus aureus in nutrient broth by mint essential oil. Food Res Int. 2000; 33(3-4): 273-280. [DOI:10.1016/S0963-9969(00)00047-8]
22. Delgado B, Fernandez PS, Palop A, periago PM. Effect of thymol and cymene on Bacillus cereus frequency distributions. Food Microbiol. 2004; 21(3): 327-334. [DOI:10.1016/S0740-0020(03)00075-3]
23. Valero M, Giner MJ. Effects of antimicrobial components of essential oils on growth of Bacillus cereus INRA L2104 in and the sensory qualities of carrot broth. Int J Food Microbiol. 2006; 106(1):90-4. [DOI:10.1016/j.ijfoodmicro.2005.06.011]
24. Smith P, Stewart J, Fyfe L. The potential application of plant essential oils as natural food preservatives in soft cheese. Food Microbiol. 2001; 18(4): 463-470. [DOI:10.1006/fmic.2001.0415]
25. Chami N, Chami F, Bennis S, Trouillas J, Remmal A. Antifungal treatment with carvacrol and eugenol of oral candidiasis in immunosuppressed rats. Braz J Infec Dis. 2004; 8(3): 217-226. [DOI:10.1590/S1413-86702004000300005]
26. Aghajani Z, Assadian F, Masoudi Sh, Chalabian F, Esmaeili A, Tabatabaei M, Rustaiyan A. Chemical composition and in vitro antibacterial activities of the oil of Ziziphora clinopodioides and Z. capitata from Iran. Chemistry of Natural Compounds. 2008; 44(3):387-389. [DOI:10.1007/s10600-008-9073-4]
27. Amiri H. Composition and antioxidant activity of the essential oil and methanolic extract of Ziziphora clinopodioides Lam. in preflowering stage. Journal of Kerman University of Medical Sciences. 2009; 16(1): 79-86.
28. Mahboubi M, Bokaee S, Dehdashti H, Feizabadi MM. Antimicrobial activity of Mentha piperitae, Zhumeria majdae, Ziziphora tenuior oils on ESBLs producing isolates of Klebsiella pneumonia. Biharean Biologist. 2012; 6(1): 5-9.
29. Ozturk S, Ercisli S. The chemical composition of Essential oil and in vitro antibacterial activities of essential oils and methanol extract of Ziziphora persica bunge. J Ehtanopharmacol. 2006; 106(3): 327-376. [DOI:10.1016/j.jep.2006.01.014]
30. Ozturk S, Ercisli S. Antibacterial activity and chemical constitutions of Ziziphora clinopodioides. Food Control. 2007; 18(5): 535-540. [DOI:10.1016/j.foodcont.2006.01.002]
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