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1- Department of Sport science, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Department of Sport science, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran ,abbasi.daloii@gmail.com
3- Department of Sport science, Shahrood Branch, Islamic Azad University, Shahrood, Iran
2- Department of Sport science, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran ,
3- Department of Sport science, Shahrood Branch, Islamic Azad University, Shahrood, Iran
Abstract: (606 Views)
Background and objectives: The purpose of the present study was to investigate the effect of aerobic exercise and psilocybin after methamphetamine induction on the gene expression of some cerebral cortex semaphorins in female Wistar rats.
Methods: In this experimental study, 40 female rats were placed in five groups; control (C), amphetamine (A), amphetamine-aerobic (AA), amphetamine-psilocybin (AP), amphetamine-psilocybin-aerobic (AAP). Methamphetamine was injected at a dose of 15 mg/kg for 5 days in the morning. Psilocybin was injected at a dose of 1mg/kg. The aerobic training program included running on a treadmill at 20-25 m/min, three days a week for eight weeks. After eight weeks, the genes expression was measured by Real Time PCR method. The data were analyzed by one-way analysis of variance and Tukey's post hoc test at a significance level of P<0.05.
Results: The results showed that the average gene expression of semaphorin 3A, semaphorin 4A and semaphorin 7A in the cerebral cortex of the A group had a significant increase compared to the C group(P=0.001). AA, AP and AAP groups showed a significant decrease in the average expression of semaphorin 3A and semaphorin 4A genes compared to the A group(P=0.001). AAP group had a significant decrease in the average expression of semaphorin 3A gene compared to AA and AP groups(P=0.001). Also, the AAP group showed a significant decrease in semaphorin 7A compared to the AP group(P=0.007).
Conclusion: According to the results, aerobic training and psilocybin supplementation can help reduce semaphorins in the cerebral cortex in rats induced with methamphetamine.
Methods: In this experimental study, 40 female rats were placed in five groups; control (C), amphetamine (A), amphetamine-aerobic (AA), amphetamine-psilocybin (AP), amphetamine-psilocybin-aerobic (AAP). Methamphetamine was injected at a dose of 15 mg/kg for 5 days in the morning. Psilocybin was injected at a dose of 1mg/kg. The aerobic training program included running on a treadmill at 20-25 m/min, three days a week for eight weeks. After eight weeks, the genes expression was measured by Real Time PCR method. The data were analyzed by one-way analysis of variance and Tukey's post hoc test at a significance level of P<0.05.
Results: The results showed that the average gene expression of semaphorin 3A, semaphorin 4A and semaphorin 7A in the cerebral cortex of the A group had a significant increase compared to the C group(P=0.001). AA, AP and AAP groups showed a significant decrease in the average expression of semaphorin 3A and semaphorin 4A genes compared to the A group(P=0.001). AAP group had a significant decrease in the average expression of semaphorin 3A gene compared to AA and AP groups(P=0.001). Also, the AAP group showed a significant decrease in semaphorin 7A compared to the AP group(P=0.007).
Conclusion: According to the results, aerobic training and psilocybin supplementation can help reduce semaphorins in the cerebral cortex in rats induced with methamphetamine.
Keywords: methamphetamine, aerobic exercise, psilocybin, semaphorins, cerebral cortex tissue structure, rats
Research Article: Research Article |
Subject:
Sport Physiology
Received: 2024/04/28 | Accepted: 2024/09/8
Received: 2024/04/28 | Accepted: 2024/09/8
References
1. Deng B, Zhang Z, Zhou H, Zhang X, Niu S, Yan X, et al. MicroRNAs in Methamphetamine-Induced Neurotoxicity and Addiction. Front Pharmacol. 2022; 13: 875666. [View at Publisher] [DOI] [PMID] [Google Scholar]
2. Fleckenstein AE, Volz TJ, Riddle EL, Gibb JW, Hanson GR. New insights into the mechanism of action of amphetamines. Annu Rev Pharmacol Toxicol. 2007; 47: 681-98. [View at Publisher] [DOI] [PMID] [Google Scholar]
3. Baptista S, Lasgi C, Benstaali C, Milhazes N, Borges F, Fontes-Ribeiro C, et al. Methamphetamine decreases dentate gyrus stem cell self-renewal and shifts the differentiation towards neuronal fate. Stem Cell Res. 2014; 13(2): 329-41.
https://doi.org/10.1016/j.scr.2014.08.003 [View at Publisher] [DOI] [PMID] [Google Scholar]
4. Deng B, Zhang Z, Zhou H, Zhang X, Niu S, Yan X, et al. MicroRNAs in Methamphetamine-Induced Neurotoxicity and Addiction. Front Pharmacol. 2022; 13: 875666. [View at Publisher] [DOI] [PMID] [Google Scholar]
5. Iragavarapu-Charyulu V, Wojcikiewicz E, Urdaneta A. Semaphorins in Angiogenesis and Autoimmune Diseases: Therapeutic Targets? Front Immunol. 2020; 11: 346. [View at Publisher] [DOI] [PMID] [Google Scholar]
6. Lotfi R, Yari K. The Role of Semaphorins and their Receptors in the Immune System and their Relation to Multiple Sclerosis, The Neuroscience Journal of Shefaye Khatam. 2018; 4: 75-92. [View at Publisher] [DOI] [Google Scholar]
7. Alto LT, Terman JR. Semaphorins and their Signaling Mechanisms. Methods Mol Biol. 2017; 1493: 1-25. [View at Publisher] [DOI] [PMID] [Google Scholar]
8. Zhou Y, Gunput RA, Pasterkamp RJ. Semaphorin signaling: progress made and promises ahead. Trends Biochem Sci. 2008; 33(4): 161-70. [View at Publisher] [DOI] [PMID] [Google Scholar]
9. Koussih L, Gounni AS. Semaphorin3E/plexinD1 Axis in Asthma: What We Know So Far! Adv Exp Med Biol. 2021; 1304: 205-213. [View at Publisher] [DOI] [PMID] [Google Scholar]
10. Loy K, Fourneau J, Meng N, Denecke C, Locatelli G, Bareyre FM. Semaphorin 7A restricts serotonergic innervation and ensures recovery after spinal cord injury. Cell Mol Life Sci. 2021; 78(6): 2911-2927. [View at Publisher] [DOI] [PMID] [Google Scholar]
11. Takegahara N, Kumanogoh A. Involvement of semaphorins and their receptors in neurological diseases, Clinical and Experimental Neuroimmunology. 2010; 1(1): 33-45.
https://doi.org/10.1111/j.1759-1961.2009.00004.x [View at Publisher] [DOI] [Google Scholar]
12. Zhao Y, Feng H, Zhang Y, Zhang JV, Wang X, Liu D, et al. Current Understandings of Core Pathways for the Activation of Mammalian Primordial Follicles. Cells. 2021; 10(6): 1491.
https://doi.org/10.3390/cells10061491 [View at Publisher] [DOI] [PMID] [Google Scholar]
13. Shkooh HP ,Saghebjoo M, Nazemi S, Hedayati M. The Effect of One-Time and Two-Times Endurance Training with the Same Volume on Glial Cell-Derived Neurotrophic Factor and Nuclear Factor-kB in Sensory Roots of Spinal Cord in Diabetic Neuropathic Rats. Sport Physiology. 2019; 43: 75-90. [View at Publisher] [DOI] [Google Scholar]
14. Bahreini pour MA. Investigation the effect of low-intensity aerobic training for 10 weeks along with blood flow restriction on amount of protein BDNF in soleus and EDL muscles as well as the sciatic nerve in aged male rats. Journal of Sport and Exercise Physiology. 2019; 12(1): 59-75. [View at Publisher] [DOI] [Google Scholar]
15. G. Candow, Forbes SC, Chilibeck PD, Cornish SM, Antonio RB. Kreider, Effectiveness of creatine supplementation on aging muscle and bone: focus on falls prevention and inflammation. J. Clin. Med. 2019; 8(4): 488.
https://doi.org/10.3390/jcm8040488 [View at Publisher] [DOI] [PMID] [Google Scholar]
16. Nkadimeng SM, Nabatanzi A, Steinmann CML, Eloff JN. Phytochemical, cytotoxicity, antioxidant and anti-inflammatory effects of Psilocybe natalensis magic mushroom, Plants. 2020; 9: 1127. [View at Publisher] [DOI] [PMID] [Google Scholar]
17. Bershad AK, Preller KH, Lee R, Keedy S, Wren-Jarvis J, Bremmer MP, et al. Preliminary Report on the Effects of a Low Dose of LSD on Resting-State Amygdala Functional Connectivity. Biol Psychiatry Cogn Neurosci Neuroimaging. 2020; 5(4): 461-467. [View at Publisher] [DOI] [PMID] [Google Scholar]
18. Curtis EK. Meth mouth: a review of methamphetamine abuse and its oral manifestations. Gen Dent. 2006; 54(2): 125-9. [View at Publisher] [PMID] [Google Scholar]
19. Hibicke M, Landry AN, Kramer HM, Talman ZK, Nichols CD. Psychedelics, but Not Ketamine, Produce Persistent Antidepressant-like Effects in a Rodent Experimental System for the Study of Depression. ACS Chem Neurosci. 2020; 11(6): 864-871. [View at Publisher] [DOI] [PMID] [Google Scholar]
20. Nkadimeng SM, Steinmann CML, Eloff JN. Anti-Inflammatory Effects of Four Psilocybin-Containing Magic Mushroom Water Extracts in vitro on 15-Lipoxygenase Activity and on Lipopolysaccharide-Induced Cyclooxygenase-2 and Inflammatory Cytokines in Human U937 Macrophage Cells. J Inflamm Res. 2021; 14: 3729-3738. [View at Publisher] [DOI] [PMID] [Google Scholar]
21. Gotvaldová K, Hájková K, Borovička J, Jurok R, Cihlářová P, Kuchař M. Stability of psilocybin and its four analogs in the biomass of the psychotropic mushroom Psilocybe cubensis, Drug testing and analysis. 2021; 2: 439-446. [View at Publisher] [DOI] [PMID] [Google Scholar]
22. Zhang KK, Wang H, Qu D, Chen LJ, Wang LB, Li JH, et al. Luteolin Alleviates Methamphetamine-Induced Hepatotoxicity by Suppressing the p53 Pathway-Mediated Apoptosis, Autophagy, and Inflammation in Rats. Front Pharmacol. 2021; 12: 641917. [View at Publisher] [DOI] [PMID] [Google Scholar]
23. Wang J, Liang M, Shang Q, Qian H, An R, Liu H, et al. Psilocin suppresses methamphetamine-induced hyperlocomotion and acquisition of conditioned place preference via D2R-mediated ERK signaling. CNS Neurosci Ther. 2023; 29(3): 831-841. [View at Publisher] [DOI] [PMID] [Google Scholar]
24. Marques E, Vasconcelos F, Rolo MR, Pereira FC, Silva AP, Macedo TR, et al. Influence of chronic exercise on the amphetamine-induced dopamine release and neurodegeneration in the striatum of the rat. Ann N Y Acad Sci. 2008; 1139: 222-31. [View at Publisher] [DOI] [PMID] [Google Scholar]
25. Gonçalves JR. Como fazer um projeto de pesquisa de um artigo de revisão de literature, Revista JRG de Estudos Acadêmicos. 2019; 5 01-28. [View at Publisher] [DOI] [Google Scholar]
26. Robbins B, Perry J, Long M, Carpenter RE. Analysis of D- and L- Isomers of (Meth)amphetamine in Human K2EDTA Plasma. Journal of Biomedical and Life Sciences. 2023; 3(1): 1–12. [View at Publisher] [DOI] [Google Scholar]
27. Van Battum EY, Gunput RA, Lemstra S, Groen EJ, Yu KL, Adolfs Y, et al. The intracellular redox protein MICAL-1 regulates the development of hippocampal mossy fibre connections. Nat Commun. 2014; 5: 4317. [View at Publisher] [DOI] [PMID] [Google Scholar]
28. Barcelo-Bovea V, Dominguez-Martinez I, Joaquin-Ovalle F, Amador LA, Castro-Rivera E, Medina-Álvarez K, et al. Optimization and Characterization of Protein Nanoparticles for the Targeted and Smart Delivery of Cytochrome c to Non-Small Cell Lung Carcinoma. Cancers (Basel). 2020; 12(5): 1215. [View at Publisher] [DOI] [PMID] [Google Scholar]
29. Moretti S, Procopio A, Lazzarini R, Rippo MR, Testa R, Marra M, Tamagnone L, Catalano A. Semaphorin3A signaling controls Fas (CD95)-mediated apoptosis by promoting Fas translocation into lipid rafts. Blood. 2008; 111(4): 2290-9. [View at Publisher] [DOI] [PMID] [Google Scholar]
30. Fazelzadeh M, Afzalpour ME, Fallah Mohammadi Z, Falah Mohammadi H. The effects of voluntary complex and regular wheel running exercises on the levels of 8-oxoguanine DNA glycosylase, semaphorin 3B, H2O2, and apoptosis in the hippocampus of diabetic rats. Brain Behav. 2021; 11(3): e01988. [View at Publisher] [DOI] [PMID] [Google Scholar]
31. Radak Z, Toldy A, Szabo Z, Siamilis S, Nyakas C, Silye G, Jakus J, Goto S. The effects of training and detraining on memory, neurotrophins and oxidative stress markers in rat brain. Neurochem Int. 2006; 49(4): 387-92. [View at Publisher] [DOI] [PMID] [Google Scholar]
32. van Praag H. Exercise and the brain: something to chew on. Trends Neurosci. 2009; 32(5): 283-90. [View at Publisher] [DOI] [PMID] [Google Scholar]
33. Kwak HB, Thalacker-Mercer A, Anderson EJ, Lin CT, Kane DA, Lee NS, et al. Simvastatin impairs ADP-stimulated respiration and increases mitochondrial oxidative stress in primary human skeletal myotubes. Free Radic Biol Med. 2012; 52(1): 198-207. [View at Publisher] [DOI] [PMID] [Google Scholar]
34. M Alipour. The effect of intellectual capital on firm performance: an investigation of Iran insurance companies, Measuring Business Excellence. 2012; 1: 53-66. [View at Publisher] [DOI] [Google Scholar]
35. Ghadiri Hormati L, Aminaei M, Dakhili A B, Asadi shekaari M. The Effect of High-Intensity Exercise Training on Gene Expression of Semaphorin 3A in Extensor Digitorum Longus Muscles of Aged C57bl/6 Mice . J. Ilam Uni. Med. Sci. 2017; 25 (1) :92-102 [View at Publisher] [DOI] [Google Scholar]
36. Lieberz J, Shamay-Tsoory SG, Saporta N, Esser T, Kuskova E, Stoffel-Wagner B, et al. Loneliness and the Social Brain: How Perceived Social Isolation Impairs Human Interactions. Adv Sci (Weinh). 2021; 8(21): e2102076.
https://doi.org/10.1002/advs.202102076 [View at Publisher] [DOI] [PMID] [Google Scholar]
37. Gunaydin LA, Deisseroth K. Dopaminergic Dynamics Contributing to Social Behavior. Cold Spring Harb Symp Quant Biol. 2014;79:221-7. [View at Publisher] [DOI] [PMID] [Google Scholar]
38. Wang Z, Zheng R, Wang X, Huang X, Huang J, Gu C, et al. Aerobic Exercise Improves Methamphetamine-Induced Olfactory Dysfunction Through α-Synuclein Intervention in Male Mice. Front Mol Neurosci. 2022; 15: 884790. [View at Publisher] [DOI] [PMID] [Google Scholar]
39. Xu J, Zhu Z, Jin Y, Wei C, Wang Y, Li X. Effect of aerobic exercise on brain metabolite profiles in the mouse models of methamphetamine addiction: LC-MS-based metabolomics study. BMC Psychiatry. 2023; 23(1): 852. [View at Publisher] [DOI] [PMID] [Google Scholar]
40. Coppola M, Bevione F, Mondola R. Psilocybin for Treating Psychiatric Disorders: A Psychonaut Legend or a Promising Therapeutic Perspective? J Xenobiot. 2022; 12(1): 41-52. [View at Publisher] [DOI] [PMID] [Google Scholar]
41. Ross S, Bossis A, Guss J, Agin-Liebes G, Malone T, Cohen B, et al. Rapid and sustained symptom reduction following psilocybin treatment for anxiety and depression in patients with life-threatening cancer: a randomized controlled trial. J Psychopharmacol. 2016; 30(12): 1165-1180.
https://doi.org/10.1177/0269881116675512 [View at Publisher] [DOI] [PMID] [Google Scholar]
42. M. P. Bogenschutz, A. A. Forcehimes, J. A. Pommy, C. E. Wilcox, P. C. R. Barbosa, R. J Strassman, Psilocybin-assisted treatment for alcohol dependence: a proof-of-concept study, Journal of psychopharmacology. 3 (2015) 289-299,
https://doi.org/10.1177/0269881114565144 [DOI] [PMID]
43. du Jardin KG, Liebenberg N, Cajina M, Müller HK, Elfving B, Sanchez C, Wegener G. S-Ketamine Mediates Its Acute and Sustained Antidepressant-Like Activity through a 5-HT1B Receptor Dependent Mechanism in a Genetic Rat Model of Depression. Front Pharmacol. 2018; 8: 978. [View at Publisher] [DOI] [PMID] [Google Scholar]
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