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1- Assistant professor, Department of Biochemistry, Siddartha medical college, Vijayawada, Andhrapradesh, India. , mahendran.kanumuru@gmail.com
2- Professor, Department of Biochemistry, Siddartha medical college, Vijayawada, Andhrapradesh, India
2- Professor, Department of Biochemistry, Siddartha medical college, Vijayawada, Andhrapradesh, India
Abstract: (487 Views)
Background: Type2 Diabetes mellitus (T2DM) is non communicable disease including hyperinsulinemia, insulin resistance, hyperglycemia and low grade chronic inflammation associated with various micro and macro vascular complications .
Objectives: The present study aimed to estimate the Vitamin D , total antioxidant capacity and Malondialdehyde (MDA) levels in T2DM patients compared with healthy volunteers, further to find out vitamin D association between HbA1c, total antioxidant capacity , MDA, lipid profile levels
Materials and methods: Seventy type 2 diabetic patients with age group of 35 to 50 years were selected and 70 healthy age matched subjects were selected as controls. Serum Vitamin D and Insulin was estimated by ELISA, Glycosylated hemoglobin (HbA1C) was assessed by HPLC method and other routine lipid profile investigations carried out by Beckman Coulter fully automated analyzer.
Results: Vitamin D levels were significantly decreased in type 2 diabetic patients. HbA1C and insulin resistance values are significantly increased in type 2 diabetic patients. Vitamin D levels Negatively correlated with MDA, insulin resistance , HbA1c and positively correlated with total antioxidant capacity . But there is no significant correlative changes observed between lipid profile parameters.
Conclusion: Vitamin D deficiency may be one of the vital risk factor responsible for increased oxidative stress in T2DM patients. Regular monitoring and supplementation of vitamin D beneficial for reduction of oxidative stress and vascular complications in T2DM patients.
Objectives: The present study aimed to estimate the Vitamin D , total antioxidant capacity and Malondialdehyde (MDA) levels in T2DM patients compared with healthy volunteers, further to find out vitamin D association between HbA1c, total antioxidant capacity , MDA, lipid profile levels
Materials and methods: Seventy type 2 diabetic patients with age group of 35 to 50 years were selected and 70 healthy age matched subjects were selected as controls. Serum Vitamin D and Insulin was estimated by ELISA, Glycosylated hemoglobin (HbA1C) was assessed by HPLC method and other routine lipid profile investigations carried out by Beckman Coulter fully automated analyzer.
Results: Vitamin D levels were significantly decreased in type 2 diabetic patients. HbA1C and insulin resistance values are significantly increased in type 2 diabetic patients. Vitamin D levels Negatively correlated with MDA, insulin resistance , HbA1c and positively correlated with total antioxidant capacity . But there is no significant correlative changes observed between lipid profile parameters.
Conclusion: Vitamin D deficiency may be one of the vital risk factor responsible for increased oxidative stress in T2DM patients. Regular monitoring and supplementation of vitamin D beneficial for reduction of oxidative stress and vascular complications in T2DM patients.
Research Article: Research Article |
Subject:
Biochemistry
Received: 2023/06/20 | Accepted: 2024/02/28
Received: 2023/06/20 | Accepted: 2024/02/28
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