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Saei Hamedani R, khanzadi S, hashemi M, azizzadeh M. Effects of Alginate Coating and Neutral Electrolyzed Water on E. coli O157:H7 Contamination of Salmon Fillets. mljgoums 2022; 16 (1) :20-24
URL: http://mlj.goums.ac.ir/article-1-1385-en.html
Effects of Alginate Coating and Neutral Electrolyzed Water on E. coli O157:H7 Contamination of Salmon Fillets
Romina Saei Hamedani1 , Saeid Khanzadi 2, Mohammad Hashemi3 , Mohammad Azizzadeh4
1- Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
2- Department of Food Hygiene and Aquaculture, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran , khanzadi@um.ac.ir
3- Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
4- Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Iran
Abstract:   (1202 Views)
Background and objectives: Neutralized electrolyzed water (NEW) is a novel natural disinfectant. It has been suggested that application of NEW can improve the shelf life of fish. This study aimed to investigate effect of NEW incorporated in alginate coating on growth of Escherichia coli O157: H7 on salmon fillets over a period of 12 days.
Methods: Fish fillets were inoculated with E. coli O157:H7 and divided into six different treatment groups: control (no coating), distilled water, alginate, EW, EW & alginate (Samples coated with alginate solution prepared by EW), and EW+ alginate (samples immersed in EW, then coated with alginate solution). The fillets were kept at 4 °C, and the bacterial count was determined on days: 0, 2, 4, 8, and 12. Data analysis was performed using repeated ANOVA and Bonferroni post-hoctest at statistical significance of 0.05.
Results: Treatment with alginate coating and EW alone could significantly reduce E. coli O157: H7 count on the salmon fillets. However, maximum reduction (1.27 log CFU/g) of bacteria was achieved when using alginate coating combined with EW.
Conclusion: According to the results, the combination of alginate coating with EW can be applied as a natural antimicrobial for increasing safety of food products, especially fish, against pathogenic bacteria such as E. coli O157: H7.
Keywords: Alginates [MeSH], Escherichia coli O157 [MeSH], Salmon [MeSH]
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Research Article: Research Article | Subject: Microbiology
Received: 2021/05/1 | Accepted: 2021/06/4 | Published: 2021/12/29 | ePublished: 2021/12/29
References
1. Heydari R, Bavandi S, Javadian SR. Effect of sodium alginate coating enriched with horsemint (M entha longifolia) essential oil on the quality of bighead carp fillets during storage at 4 C. Food science & nutrition. 2015;3(3):188-94. [View at Publisher] [DOI:10.1002/fsn3.202] [PubMed] [Google Scholar]
2. Tilahun A, Engdawork A. Isolation, Identification and Antimicrobial Susceptibility Profile of E. Coli (O157: H7) from Fish in Lake Hawassa, Southern Ethiopia. Life Science Journal. 2020; 17(2): 64-72. [View at Publisher] [DOI] [Google Scholar]
3. Mead PS, Griffin PM. Escherichia coli O157: H7. The Lancet. 1998; 352(9135): 1207-12. [View at Publisher] [DOI:10.1016/S0140-6736(98)01267-7] [PubMed] [Google Scholar]
4. Lim JY, Yoon JW, Hovde CJ. A brief overview of Escherichia coli O157: H7 and its plasmid O157. Journal of microbiology and biotechnology. 2010; 20(1): 5-14. [View at Publisher] [DOI:10.4014/jmb.0908.08007] [PubMed] [Google Scholar]
5. Rojas-Graü MA, Raybaudi-Massilia RM, Soliva-Fortuny RC, Avena-Bustillos RJ, McHugh TH, Martín-Belloso O. Apple puree-alginate edible coating as carrier of antimicrobial agents to prolong shelf-life of fresh-cut apples. Postharvest biology and Technology. 2007;45(2):254-64. [View at Publisher] [DOI:10.1016/j.postharvbio.2007.01.017] [Google Scholar]
6. Wang Z, Hu S, Gao Y, Ye C, Wang H. Effect of collagen-lysozyme coating on fresh-salmon fillets preservation. LWT. 2017;75:59-64. [View at Publisher] [DOI:10.1016/j.lwt.2016.08.032] [Google Scholar]
7. Ruan C, Zhang Y, Sun Y, Gao X, Xiong G, Liang J. Effect of sodium alginate and carboxymethyl cellulose edible coating with epigallocatechin gallate on quality and shelf life of fresh pork. International journal of biological macromolecules. 2019;141:178-84. [View at Publisher] [DOI:10.1016/j.ijbiomac.2019.08.247] [PubMed] [Google Scholar]
8. Kazemi SM, Rezaei M. Antimicrobial effectiveness of gelatin-alginate film containing oregano essential oil for fish preservation. Journal of food safety. 2015; 35(4): 482-90. [View at Publisher] [DOI:10.1111/jfs.12198] [Google Scholar]
9. Comaposada J, Gou P, Marcos B, Arnau J. Physical properties of sodium alginate solutions and edible wet calcium alginate coatings. LWT-Food Science and Technology. 2015;64(1):212-9. [View at Publisher] [DOI:10.1016/j.lwt.2015.05.043] [Google Scholar]
10. Fabrizio K, Sharma R, Demirci A, Cutter C. Comparison of electrolyzed oxidizing water with various antimicrobial interventions to reduce Salmonella species on poultry. Poultry science. 2002; 81(10): 1598-605. [View at Publisher] [DOI:10.1093/ps/81.10.1598] [PubMed] [Google Scholar]
11. Zhou R, Liu Y, Xie J, Wang X. Effects of combined treatment of electrolysed water and chitosan on the quality attributes and myofibril degradation in farmed obscure puffer fish (Takifugu obscurus) during refrigerated storage. Food Chemistry. 2011; 129(4): 1660-6. [View at Publisher] [DOI:10.1016/j.foodchem.2011.06.028] [Google Scholar]
12. Phuvasate S, Su Y-C. Effects of electrolyzed oxidizing water and ice treatments on reducing histamine-producing bacteria on fish skin and food contact surface. Food control. 2010;21(3):286-91. [View at Publisher] [DOI:10.1016/j.foodcont.2009.06.007] [Google Scholar]
13. Al-Holy MA, Rasco BA. The bactericidal activity of acidic electrolyzed oxidizing water against Escherichia coli O157: H7, Salmonella Typhimurium, and Listeria monocytogenes on raw fish, chicken and beef surfaces. Food Control. 2015; 54: 317-21. [View at Publisher] [DOI:10.1016/j.foodcont.2015.02.017] [Google Scholar]
14. Jadeja R, Hung Y-C. Efficacy of near neutral and alkaline pH electrolyzed oxidizing waters to control Escherichia coli O157: H7 and Salmonella Typhimurium DT 104 from beef hides. Food Control. 2014;41:17-20. [View at Publisher] [DOI:10.1016/j.foodcont.2013.12.030] [Google Scholar]
15. Hao J, Qiu S, Li H, Chen T, Liu H, Li L. Roles of hydroxyl radicals in electrolyzed oxidizing water (EOW) for the inactivation of Escherichia coli. International journal of food microbiology. 2012;155(3):99-104. [View at Publisher] [DOI:10.1016/j.ijfoodmicro.2011.12.031] [PubMed] [Google Scholar]
16. Alavi SH, Khanzadi S, Hashemi M, Azizzadeh M. The Effects of Alginate Coatings Containing Zataria multiflora Boiss Essential Oil in the Forms of Coarse Emulsion and Nano-emulsion on Inoculated Escherichia coli O157: H7 in Beef Fillets. Journal of Nutrition, Fasting and Health. 2020;8(2):94-9. [View at Publisher] [DOI] [Google Scholar]
17. Sharifi F, Khanzadi S, Hashemi M, Azizzadeh M. Control of Listeria monocytogenes and Escherichia coli O157: H7 inoculated on fish fillets using alginate coating containing lactoperoxidase system and Zataria multiflora boiss essential oil. Journal of aquatic food product technology. 2017; 26(9): 1014-21. [View at Publisher] [DOI:10.1080/10498850.2017.1375057] [Google Scholar]
18. Bialka KL, Demirci A, Knabel S, Patterson P, Puri V. Efficacy of electrolyzed oxidizing water for the microbial safety and quality of eggs. Poultry Science. 2004; 83(12): 2071-8. [View at Publisher] [DOI:10.1093/ps/83.12.2071] [PubMed] [Google Scholar]
19. Abadias M, Usall J, Oliveira M, Alegre I, Viñas I. Efficacy of neutral electrolyzed water (NEW) for reducing microbial contamination on minimally-processed vegetables. International journal of food microbiology. 2008; 123(1-2): 151-8. [View at Publisher] [DOI:10.1016/j.ijfoodmicro.2007.12.008] [PubMed] [Google Scholar]
20. Deza M, Araujo M, Garrido M. Inactivation of Escherichia coli O157: H7, Salmonella enteritidis and Listeria monocytogenes on the surface of tomatoes by neutral electrolyzed water. Letters in applied microbiology. 2003; 37(6): 482-7. [View at Publisher] [DOI:10.1046/j.1472-765X.2003.01433.x] [PubMed] [Google Scholar]
21. Liu Q, Jin X, Feng X, Yang H, Fu C. Inactivation kinetics of Escherichia coli O157: H7 and Salmonella Typhimurium on organic carrot (Daucus carota L.) treated with low concentration electrolyzed water combined with short-time heat treatment. Food Control. 2019; 106: 106702. [View at Publisher] [DOI:10.1016/j.foodcont.2019.06.028] [Google Scholar]
22. Azizian A, Khanzadi S, Hashemi M, Azizzadeh M. Inhibitory Effect of Nano-gel/Emulsion of Chitosan Coating Incorporated with Ziziphora Clinopodioides Essential Oil and Nisin on Escherichia coli O157: H7 Inoculated in Beef at Cold Storage Condition. Journal of Nutrition, Fasting and Health. 2019; 7(2): 103-9. [View at Publisher] [DOI] [Google Scholar]
23. Didar H, Khanzadi S, Azizzadeh M. Effects of alginate coating incorporated with Bunium persicum essential oil and Lactoperoxidase system on inoculated Listeria monocytogenes in chicken breast fillets. Journal of Health sciences and Technology. 2018; 2(1): 9-14. [View at Publisher] [DOI:10.32592/JHST.2018.2.1.102] [Google Scholar]
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