Sat, Nov 23, 2024
Volume 16, Issue 3 (May-Jun 2022)
mljgoums 2022, 16(3): 19-23 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
roohbakhsh E, barari A, abbaszadeh H. Effects of Exercise Training and Consumption of Fenugreek Seed Extract on Expression of Oxidised LDL and ROS-related Genes in Patients with Coronary Artery Occlusion. mljgoums 2022; 16 (3) :19-23
URL: http://mlj.goums.ac.ir/article-1-1358-en.html
URL: http://mlj.goums.ac.ir/article-1-1358-en.html
1- Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran ,alireza54.barari@gmail.com
3- Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran
2- Department of Sport Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran ,
3- Department of Sport Physiology, Sari Branch, Islamic Azad University, Sari, Iran
Abstract: (1578 Views)
Background and objectives: Sedentary life style and high-fat diet are one of the key causes of coronary artery disease. The aim of this study was to evaluate effects of periodic exercise and consumption of fenugreek seed extract on the expression of oxidized low-density lipoprotein (oxLDL) and reactive oxygen species (ROS) genes in patients with coronary artery occlusion.
Methods: The present quasi-experimental study was performed on 32 men (60±5 years) with coronary artery disease. The subjects were randomly divided into four groups: control, exercise, fenugreek supplementation, and combination of exercise and fenugreek supplementation. The training program consisted of eight weeks of intermittent running at 55 to 65% heart rate reserve, three sessions per week, with an emphasis on gradual overload. The subjects consumed 10 mg/kg of body weight fenugreek extract daily. Data analysis was performed using two-way analysis of variance and Tukey's test at statistical significance of 0.05.
Results: The mean expression ratio of oxLDL and ROS genes in the intervention groups decreased significantly (p<0.0001). Compared with the control group, the most profound decreased in oxLDL and ROS expression occurred after combination of exercise and fenugreek supplementation (p<0.0001).
Conclusion: The results showed that intermittent exercise and consumption of fenugreek seed extract might have beneficial effects on the antioxidant defense system in patients with coronary artery occlusion.
Methods: The present quasi-experimental study was performed on 32 men (60±5 years) with coronary artery disease. The subjects were randomly divided into four groups: control, exercise, fenugreek supplementation, and combination of exercise and fenugreek supplementation. The training program consisted of eight weeks of intermittent running at 55 to 65% heart rate reserve, three sessions per week, with an emphasis on gradual overload. The subjects consumed 10 mg/kg of body weight fenugreek extract daily. Data analysis was performed using two-way analysis of variance and Tukey's test at statistical significance of 0.05.
Results: The mean expression ratio of oxLDL and ROS genes in the intervention groups decreased significantly (p<0.0001). Compared with the control group, the most profound decreased in oxLDL and ROS expression occurred after combination of exercise and fenugreek supplementation (p<0.0001).
Conclusion: The results showed that intermittent exercise and consumption of fenugreek seed extract might have beneficial effects on the antioxidant defense system in patients with coronary artery occlusion.
Research Article: Original Paper |
Subject:
Sport Physiology
Received: 2021/01/17 | Accepted: 2021/03/1 | Published: 2022/05/14 | ePublished: 2022/05/14
Received: 2021/01/17 | Accepted: 2021/03/1 | Published: 2022/05/14 | ePublished: 2022/05/14
References
1. Subasi S, Geleccek N, Ozdemir N, Ormen M. Influence of acute resistance and aerobic exercise on plasma homocysteine level and lipid profiles. Turk J Biochem. 2009; 34(1): 9-14.
2. DehghanSH, SharifiGH, Faramarzi M. The effect of eight week low impact rhythmic aerobic training on total plasma homocysteine concentration in older non-athlete women. J MzandaranUniv Med Sci 2009; 19(72): 54-59. [View at Publisher] [Google Scholar]
3. Jabery A, Jazayery A, Mohagheghi A, Rahimi A. Blood homocysteine enhancement in 35- 65 ischemic stricken patience. J Hygiene Res Center 2003; 7(3): 63-67.
4. Gill JM, Caslake MJ, McAllister C, Tsofliou F, Ferrell WR, Packard CJ, et al. Effects of short-term detraining on postprandial metabolism, endothelial function, and inflammation in endurance-trained men: dissociation between changes in triglyceride metabolism and endothelial function. j.gill@bio.gla.ac.uk. J Clin Endocrinol Metab. 2003; 88(9): 4328-35. [View at Publisher] [DOI:10.1210/jc.2003-030226] [PubMed] [Google Scholar]
5. Ridker PM , Rifai N , Rose L , Buring JE , Cook NR. Comparison of C-reactive protein and low-density lipoprotein cholesterol levels in the prediction of first cardiovascular events. New England Journal of Medicine. 2002; 347(20): 1557-1565. [View at Publisher] [DOI:10.1056/NEJMoa021993] [PubMed] [Google Scholar]
6. Abdullaev F. Plant-derived agents against cancer. Pharmacology and therapeutics in the new millennium. New Delhi: Narosa Publishing House. 2001; 345-354. [Google Scholar]
7. KamaliSarvestani A. The combined effect of aerobic exercise and fenugreek supplementation on the lipid profile of inactive obese women, M.Sc. Thesis, 2013
8. Burgomaster KA, Howarth KR, Phillips SM, Rakobowchuk M, Macdonald MJ, McGee SL, et al. Similar metabolic adaptations during exercise after low volume sprint interval and traditional endurance training in humans. J Physiol. 2008; 586: 151-60. [View at Publisher] [DOI:10.1113/jphysiol.2007.142109] [PubMed] [Google Scholar]
9. GibalaMJ. High-intensity interval training: New insights. Chinese Sports Medicine. 2008; (3):31.
10. Steinberg D, Parthasarathy S, Carew TE, KhooJC, Witztum JL. Beyond cholesterol: Modifications of lowdensity lipoprotein that increase its atherogenicity. N Engl J Med. 1989; 320: 915-24. [View at Publisher] [DOI:10.1056/NEJM198904063201407] [PubMed]
11. HeineckeJW. Oxidants and antioxidants in the pathogenesis of atherosclerosis: implications for the oxidized low density lipoprotein hypothesis. Atherosclerosis. 1998; 141: 1-15. [View at Publisher] [DOI:10.1016/S0021-9150(98)00173-7] [PubMed] [Google Scholar]
12. Yla-Herttuala S. Is oxidized low-density lipoprotein present in vivo? CurrOpinLipidol. 1998; 9: 337-44. [View at Publisher] [DOI:10.1097/00041433-199808000-00009] [PubMed] [Google Scholar]
13. Brown MS, Goldstein JL. Lipoprotein metabolism in the macrophage: implications for cholesterol disposition in atherosclerosis. Annu Rev Biochem 1983; 52: 223-61. [View at Publisher] [DOI:10.1146/annurev.bi.52.070183.001255] [Google Scholar]
14. Chisolm GM, Hazen SL, Fox PL, Cathcart MK. The oxidation of lipoproteins by monocytes-macrophages.Biochemical and biological mechanisms. J BiolChem. 1999; 274: 25959-62. [View at Publisher] [DOI:10.1074/jbc.274.37.25959] [PubMed] [Google Scholar]
15. Holvoet P, Vanhaecke J, Janssens S, Van de WerfF,Collen D. Oxidized LDL and malondialdehyde-modified LDL in patients with acute coronary syndromes and stable coronary artery disease. Circulation 1998; 98:1487-94. [View at Publisher] [DOI:10.1161/01.CIR.98.15.1487] [PubMed] [Google Scholar]
16. Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation,more stable ABTS radical cation. ClinBiochem. 2004; 37: 277-85. [View at Publisher] [DOI:10.1016/j.clinbiochem.2003.11.015] [PubMed] [Google Scholar]
17. Young IS, Woodside IV. Antioxidants in health and disease. J ClinPathol; 2001. 54: 176-86. [View at Publisher] [DOI:10.1136/jcp.54.3.176] [PubMed] [Google Scholar]
18. Clarkson PM, Thompson HS. Antioxidants: what role do they play in physical activity and health? Am J ClinNutr. 2000; 72: 637S-46S. [View at Publisher] [DOI:10.1093/ajcn/72.2.637S] [PubMed] [Google Scholar]
19. Wang JS, Lin CC, Chen JK, Wong MK. Role of chronic exercise in decreasing oxidized LDL-potentiated platelet activation by enhancing platelet-derived no release and bioactivity in rats. Life Sci. 2000; 66: 1937-48. [View at Publisher] [DOI:10.1016/S0024-3205(00)00519-1] [PubMed] [Google Scholar]
20. Petit PR, Sauvaire YD, Hillaire-Buys DM, Leconte OM, Baissac YG, Ponsin G, et al. Steroidsaponins from fenugreek seeds: extraction, purification, and pharmacological investigation on feeding behavior and plasma cholesterol. Steroids.1995; 60(10): 674-680. [View at Publisher] [DOI:10.1016/0039-128X(95)00090-D] [PubMed] [Google Scholar]
21. Yagi K. Lipid peroxides and human diseases. ChemPhys Lipids. 1987; 45: 337-51. [View at Publisher] [DOI:10.1016/0009-3084(87)90071-5] [PubMed] [Google Scholar]
22. Buetner GR, Schafer FQ. Free radicals, oxidants and antioxidants. Teratology. 2000; 62: 234-38. [View at Publisher] [DOI:10.1002/1096-9926(200010)62:43.0.CO;2-9] [PubMed] [Google Scholar]
23. Rumley AG, Paterson JR. Analytical aspects of antioxidants and free radical activity inclinical biochemistry. Ann ClinBiochem; 1998. 35: 181-200. [DOI:10.1177/000456329803500202] [PubMed] [Google Scholar]
24. William D, Mc Ardle-Frank I. Katch. Exercise Physiology. 2nd ed. Philadelphia: Lippincott. 2007; 72-4.
25. Akams A, Best TM. The role of anti oxidants in exercise and disease prevention. Med Sci Sports Exerc;2002. 30(5): 265-71. [View at Publisher] [DOI:10.3810/psm.2002.05.281] [PubMed] [Google Scholar]
26. Hodgson EK, Fridovich I. The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide: inactivation of the enzyme. Biochemistry. 1975; 14(24): 5294-9. [DOI:10.1021/bi00695a010] [PubMed]
27. Naghibi S, Maleki J. The effect of exercise training on anaerobic threshold and exercise tolerance in patients with coronary artery disease. medical social. 2011; 17-33. [Google Scholar]
28. Srimahachota S, Wunsuwan R, Siritantikorn A, Boonla C, Chaiwongkarjohn S, Tosukhowong P. Effects of lifestyle modification on oxidized LDL, reactive oxygen species production and endothelial cell viability in patients with coronary artery disease. ClinBiochem. 2010; 43: 858-62. [View at Publisher] [DOI:10.1016/j.clinbiochem.2010.04.056] [PubMed] [Google Scholar]
29. Lloyd PG, Prior BM, Yang HT, TerjungRL. Angiogenic growth factor expression in rat skeletal muscle in response to exercise training. American Journal of Physiology-Heart and Circulatory Physiology. 2003; 284(5): H1668-H78. [View at Publisher] [DOI:10.1152/ajpheart.00743.2002] [PubMed] [Google Scholar]
30. Attaran M, Pas qualotto E, Margaritas I, Palazzetti S, Rousseau AS. Antioxidants supplementation and tapering exercise, improve exercise-induced antioxidant response. Am J Nutrition. 2003; 22(2): 147-56. [View at Publisher] [DOI:10.1080/07315724.2003.10719288] [PubMed] [Google Scholar]
31. Emtiazy M, Oveidzadeh L, Habibi M, Molaeipour L, Talei D, Parvin M, et al. Investigating the effectiveness of the Trigonellafoenum-graecum L.(fenugreek) seeds in mild asthma: A randomized controlled trial. Allergy, Asthma & Clinical Immunology. 2018; 14(1):19. [View at Publisher] [DOI:10.1186/s13223-018-0238-9] [PubMed] [Google Scholar]
32. Arshadi S, Azarbayjani MA, Hajiaghaalipour F, Yusof A, Peeri M, Bakhtiyari S, et al. Evaluation of Trigonellafoenum-graecum extract in combination with swimming exercise compared to glibenclamide consumption on type 2 Diabetic rodents. Food & nutrition research. 2015; 59(1): 29717. [View at Publisher] [DOI:10.3402/fnr.v59.29717] [PubMed] [Google Scholar]
33. yousefi E, Zavoshy R, Noroozi M, Jahani Hashemi H, Zareiy S, Alizade K et al . Effect of oral administration of fenugreek seeds powdered on lipid profile. EBNESINA. 2015; 17 (1) :33-38 [View at Publisher] [Google Scholar]
34. GohZhong Sheng J. Effects of curcumin and fenugreek soluble fiber supplements on submaximal and maximal aerobic performance indices in untrained college-aged subjects. UKnowledge. 2019. [View at Publisher] [DOI:10.3390/jfmk5020034] [Google Scholar]
35. Nisari M, Alpa S, Yilmaz S, Inanc N. Effects of Fenugreek Extract on Total Antioxidant/Oxidant Status at Ehrlich Ascites Tumor Bearing Mice. EJMI. 2020; 4(1):116-122. [DOI:10.14744/ejmi.2020.17680] [Google Scholar]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.