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Arefi M, Abdollahi A, Khosravi A, Moradi A, Aghaee-Bakhtiari S H, Javid N, et al . Expression Analysis of Circulating miR-21 in Iranian Patients with Hereditary Colorectal Cancer. mljgoums 2021; 15 (2) :1-4
URL: http://mlj.goums.ac.ir/article-1-1262-en.html
URL: http://mlj.goums.ac.ir/article-1-1262-en.html
Mohammad Arefi1 
, Abbas Abdollahi2 , Ayyoob Khosravi3 , Abdolavahab Moradi4 , Seyed Hamid Aghaee-Bakhtiari5 , Naimeh Javid4 , Mehdi Evazalipour6 , Anvarsadat Kianmehr 7
, Abbas Abdollahi2 , Ayyoob Khosravi3 , Abdolavahab Moradi4 , Seyed Hamid Aghaee-Bakhtiari5 , Naimeh Javid4 , Mehdi Evazalipour6 , Anvarsadat Kianmehr 7
1- Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
2- Department of General Surgery, School of Medicine, Surgical Oncology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
3- Department of Molecular Medicine, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
4- Department of Microbiology, School of Medicine, Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran
5- Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6- Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
7- Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran ,kiabiotpro@yahoo.com
2- Department of General Surgery, School of Medicine, Surgical Oncology Research Center, Ghaem Hospital, Mashhad University of Medical Sciences, Mashhad, Iran
3- Department of Molecular Medicine, School of Advanced Technologies in Medicine, Golestan University of Medical Sciences, Gorgan, Iran
4- Department of Microbiology, School of Medicine, Research Center of Gastroenterology and Hepatology, Golestan University of Medical Sciences, Gorgan, Iran
5- Department of Medical Biotechnology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
6- Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran
7- Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran ,
Abstract: (3128 Views)
Background and objectives: Colorectal cancer (CRC) is one of the leading causes of cancer-related mortality in the world. MicroRNAs (miRNAs) have potential as diagnostic biomarkers for various diseases including cancer. This study was undertaken to investigate expression of miR-21 before and after surgery in patients with hereditary CRC.
Methods: After collecting blood samples from 39 patients and 39 healthy controls, total RNA was extracted by the TRIzol method. Following cDNA synthesis, expression of miR-21 in serum of subjects was evaluated using real-time PCR, along with two reference genes, let-7d and let-7g. The real-time expression results and Ct values were collected and analyzed based on the 2-∆∆ct method.
Results: In spite of tumor removal, serum miR-21 expression levels was significantly higher in hereditary CRC patients compared with controls (P=0.022).
Conclusion: Our results confirmed that samples from hereditary cases of CRC must not be included in experiments on the diagnostic potential of miRNAs.
Methods: After collecting blood samples from 39 patients and 39 healthy controls, total RNA was extracted by the TRIzol method. Following cDNA synthesis, expression of miR-21 in serum of subjects was evaluated using real-time PCR, along with two reference genes, let-7d and let-7g. The real-time expression results and Ct values were collected and analyzed based on the 2-∆∆ct method.
Results: In spite of tumor removal, serum miR-21 expression levels was significantly higher in hereditary CRC patients compared with controls (P=0.022).
Conclusion: Our results confirmed that samples from hereditary cases of CRC must not be included in experiments on the diagnostic potential of miRNAs.
Keywords: Colorectal Neoplasms [MeSH], Gene Expression [MeSH], Real-Time Polymerase Chain Reaction [MeSH]
Research Article: Original Paper |
Subject:
Molecular Medicine
Received: 2019/11/5 | Accepted: 2019/12/14 | Published: 2021/02/28 | ePublished: 2021/02/28
Received: 2019/11/5 | Accepted: 2019/12/14 | Published: 2021/02/28 | ePublished: 2021/02/28
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This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.