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Volume 15, Issue 1 (Jan-Feb 2021)
mljgoums 2021, 15(1): 33-39 |
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Sadighi A, abdi A, Azarbayjani M A, barari A. Response of Some Apoptotic Indices to Six Weeks of Aerobic Training in Streptozotocin-Induced Diabetic Rats. mljgoums 2021; 15 (1) :33-39
URL: http://mlj.goums.ac.ir/article-1-1255-en.html
URL: http://mlj.goums.ac.ir/article-1-1255-en.html
1- Department of Sport physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran
2- Department of Sport physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran ,a.abdi58@gmail.com
3- Department of Sport physiology, Faculty of Physical Education and Sports Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
2- Department of Sport physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran ,
3- Department of Sport physiology, Faculty of Physical Education and Sports Science, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Abstract: (5489 Views)
Background and objectives: Cardiac apoptosis is one of the most important cardiovascular complications of diabetes. We aimed to investigate the changes of Bax, Bcl2 and caspase 3 in cardiac tissue of diabetic rats after six weeks aerobic exercise.
Methods: Thirty two male Wistar rats were randomly divided into healthy control, diabetes control and diabetes + exercise groups. Diabetes was induced by intraperitoneal injection of streptozotocin solution (55 mg/kg). Two weeks after the injection, fasting blood glucose levels were measured to confirm induction of diabetes. The exercise program was performed five days a week for six weeks. Variables were evaluated by ELISA and western blot analysis. All statistical analyses were performed in SPSS (version 22) using ANOVA and at significance of 0.05.
Results: The induction of diabetes in the control groups resulted in a significant increase in Bax, Bax/Bcl2 ratio and a significant decrease in Bcl2 levels (P=0.024). The six-week training exercise in diabetic groups significantly decreased Bax and Bax/Bcl2 ratio and significantly increased Bcl2 (P=0.018).
Conclusion: Our finding showed that diabetes could increase apoptosis in cardiac tissue. In addition, the six-week aerobic exercise can be used as a non-pharmacological strategy to reduce diabetes-related apoptosis in cardiomyocytes.
Methods: Thirty two male Wistar rats were randomly divided into healthy control, diabetes control and diabetes + exercise groups. Diabetes was induced by intraperitoneal injection of streptozotocin solution (55 mg/kg). Two weeks after the injection, fasting blood glucose levels were measured to confirm induction of diabetes. The exercise program was performed five days a week for six weeks. Variables were evaluated by ELISA and western blot analysis. All statistical analyses were performed in SPSS (version 22) using ANOVA and at significance of 0.05.
Results: The induction of diabetes in the control groups resulted in a significant increase in Bax, Bax/Bcl2 ratio and a significant decrease in Bcl2 levels (P=0.024). The six-week training exercise in diabetic groups significantly decreased Bax and Bax/Bcl2 ratio and significantly increased Bcl2 (P=0.018).
Conclusion: Our finding showed that diabetes could increase apoptosis in cardiac tissue. In addition, the six-week aerobic exercise can be used as a non-pharmacological strategy to reduce diabetes-related apoptosis in cardiomyocytes.
Research Article: Original Paper |
Subject:
Sport Physiology
Received: 2019/09/28 | Accepted: 2020/05/3 | Published: 2021/01/1 | ePublished: 2021/01/1
Received: 2019/09/28 | Accepted: 2020/05/3 | Published: 2021/01/1 | ePublished: 2021/01/1
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