Sat, Nov 23, 2024
Volume 14, Issue 3 (May-Jun 2020)
mljgoums 2020, 14(3): 40-45 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Bagheri R, Darroudi S, Hosseini S M, Nikkar H, khodadadi F, kasraee S, et al . Effects of High-Intensity Resistance Training and Aerobic Exercise on Expression of ABCG4, ABCG5 and ABCG8 Genes in Female Athletes. mljgoums 2020; 14 (3) :40-45
URL: http://mlj.goums.ac.ir/article-1-1218-en.html
URL: http://mlj.goums.ac.ir/article-1-1218-en.html
Reza Bagheri1 
, Samane Darroudi2 , S Mojtaba Hosseini3 , Hossein Nikkar4 , Fatemeh Khodadadi4 , Somayye Kasraee2 , Mahbube Sazegar3 , Amir Rashidlamir 5
, Samane Darroudi2 , S Mojtaba Hosseini3 , Hossein Nikkar4 , Fatemeh Khodadadi4 , Somayye Kasraee2 , Mahbube Sazegar3 , Amir Rashidlamir 5
1- Department of Applied Exercise Physiology at Ferdowsi University of Mashhad, Mashhad, Iran.
2- Department of Exercise Physiology, Ferdowsi University of Mashhad, Mashhad, Iran.
3- Islamic Azad University, Neyshabur Branch, Physical Education and Sports Sciences Department, Neyshabur, Iran.
4- Young Researchers and Elite Club, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
5- Department of Exercise Physiology, Ferdowsi University of Mashhad, Mashhad, Iran. ,Rashidlamir@um.ac.ir
2- Department of Exercise Physiology, Ferdowsi University of Mashhad, Mashhad, Iran.
3- Islamic Azad University, Neyshabur Branch, Physical Education and Sports Sciences Department, Neyshabur, Iran.
4- Young Researchers and Elite Club, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
5- Department of Exercise Physiology, Ferdowsi University of Mashhad, Mashhad, Iran. ,
Abstract: (4235 Views)
Background and objectives: Atherosclerotic cardiovascular disease is the leading cause of death in industrialized countries. High level of plasma lipids including cholesterol and triglycerides is one of the most important risk factors of atherosclerosis. Previous studies have shown that three members of the ATP-binding cassette, subfamily G (ABCG4, ABCG5 and ABCG8) are involved in transporting sterols across membranes. The purpose of this study was to assess the effect of high-intensity resistance training (HIRT) and aerobic exercise (AE) on expression of the ABCG4, ABCG5 and ABCG8 genes in female athletes.
Methods: Twenty-four female athletes from the Khorasan Province (Iran) were randomly selected and assigned into three groups: control (N=8), AE (N=8) and HIRT (N=8). The subjects in the HIRT group and the AE group performed exercise at intensity of 75-80% of 1-repetion maximum and 75-80% of maximum heart rate, respectively. Blood samples were collected at baseline and immediately after the exercise session. After isolation of lymphocytes by centrifugation and purification of mRNA, gene expression changes were investigated by Real-Time-PCR. Data were analyzed using one-way ANOVA and the Tukey's test.
Results: Both training protocols significantly increased the expression of ABCG4, ABCG5 and ABCG8 in the subjects (P<0.05), and there was no significant difference in the expression of these genes between the experimental groups (P>0.05).
Conclusion: A single session of HIRT and AE may have beneficial effects on prevention of atherosclerosis.
Methods: Twenty-four female athletes from the Khorasan Province (Iran) were randomly selected and assigned into three groups: control (N=8), AE (N=8) and HIRT (N=8). The subjects in the HIRT group and the AE group performed exercise at intensity of 75-80% of 1-repetion maximum and 75-80% of maximum heart rate, respectively. Blood samples were collected at baseline and immediately after the exercise session. After isolation of lymphocytes by centrifugation and purification of mRNA, gene expression changes were investigated by Real-Time-PCR. Data were analyzed using one-way ANOVA and the Tukey's test.
Results: Both training protocols significantly increased the expression of ABCG4, ABCG5 and ABCG8 in the subjects (P<0.05), and there was no significant difference in the expression of these genes between the experimental groups (P>0.05).
Conclusion: A single session of HIRT and AE may have beneficial effects on prevention of atherosclerosis.
Research Article: Original Paper |
Subject:
Molecular Medicine
Received: 2019/05/15 | Accepted: 2019/07/28 | Published: 2020/04/30 | ePublished: 2020/04/30
Received: 2019/05/15 | Accepted: 2019/07/28 | Published: 2020/04/30 | ePublished: 2020/04/30
References
1. Rashidlamir A, Saadatnia A. The Effect of Eight Weeks of Wrestling and Wrestling Technique Based Circuit Trainingon Lymphocyte ABCA1 Gene Expression and Plasma Apolipoprotein AI. World journal of sport sciences. 2011; 4(2): 144-150. [DOI:10.1080/21615667.2011.10878931]
2. Schmitz G, Kaminski WE, Orsó E. ABC transporters in cellular lipid trafficking. Current opinion in lipidology. 2000;11(5):493-501. [DOI:10.1097/00041433-200010000-00007]
3. Yancey PG, Bortnick AE, Kellner-Weibel G, De la Llera-Moya M, Phillips MC, Rothblat GH. Importance of different pathways of cellular cholesterol efflux. Arteriosclerosis, thrombosis, and vascular biology. 2003;23(5):712-9. [DOI:10.1161/01.ATV.0000057572.97137.DD]
4. Miller G, Miller N. Plasma-high-density-lipoprotein concentration and development of ischaemic heart-disease. The lancet. 1975;305(7897):16-9. [DOI:10.1016/S0140-6736(75)92376-4]
5. Woodward OM, Köttgen A, Köttgen M. ABCG transporters and disease. The FEBS journal. 2011; 278(18): 3215-25. doi: 10.1111/j.1742-4658.2011.08171.x. [DOI:10.1111/j.1742-4658.2011.08171.x]
6. Tanisawa K, Tanaka M, Higuchi M. Gene-exercise interactions in the development of cardiometabolic diseases. The Journal of Physical Fitness and Sports Medicine. 2016;5(1):25-36. [DOI:10.7600/jpfsm.5.25]
7. Fitzgerald ML, Mujawar Z, Tamehiro N. ABC transporters, atherosclerosis and inflammation. Atherosclerosis. 2010; 211(2): 361-70. doi: 10.1016/j.atherosclerosis.2010.01.011. [DOI:10.1016/j.atherosclerosis.2010.01.011]
8. Davidson AL, Chen J. ATP-binding cassette transporters in bacteria. Annual review of biochemistry. 2004;73(1):241-68. [DOI:10.1146/annurev.biochem.73.011303.073626]
9. Choi H, Jin JY, Choi S, Hwang JU, Kim YY, Suh MC, et al. An ABCG/WBC‐type ABC transporter is essential for transport of sporopollenin precursors for exine formation in developing pollen. The Plant Journal. 2011;65(2):181-93. [DOI:10.1111/j.1365-313X.2010.04412.x]
10. Ghanbari-Niaki A, Rahmati-Ahmadabad S. Effects of a fixed-intensity of endurance training and pistacia atlantica supplementation on ATP-binding cassette G4 expression. Chinese medicine. 2013; 8(1): 23. doi: 10.1186/1749-8546-8-23. [DOI:10.1186/1749-8546-8-23]
11. Kusuhara H, Sugiyama Y. ATP-binding cassette, subfamily G (ABCG family). Pflügers Archiv-European Journal of Physiology. 2007;453(5):735-44. [DOI:10.1007/s00424-006-0134-x]
12. Durstine JL, Grandjean PW, Davis PG, Ferguson MA, Alderson NL, DuBose KD. Blood lipid and lipoprotein adaptations to exercise. Sports medicine. 2001;31(15):1033-62. DOI: 10.2165/00007256-200131150-00002. [DOI:10.2165/00007256-200131150-00002]
13. Shulenin S, Schriml L, Remaley A, Fojo S, Brewer B, Allikmets R, et al. An ATP-binding cassette gene (ABCG5) from the ABCG (White) gene subfamily maps to human chromosome 2p21 in the region of the Sitosterolemia locus. Cytogenetic and Genome Research. 2001;92(3-4):204-8. [DOI:10.1159/000056903]
14. Connolly PH, Caiozzo VJ, Zaldivar F, Nemet D, Larson J, Hung S-p, et al. Effects of exercise on gene expression in human peripheral blood mononuclear cells. Journal of applied physiology. 2004;97(4):1461-9. [DOI:10.1152/japplphysiol.00316.2004]
15. Hoang A, Tefft C, Duffy SJ, Formosa M, Henstridge DC, Kingwell BA, et al. ABCA1 expression in humans is associated with physical activity and alcohol consumption. Atherosclerosis. 2008; 197(1): 197-203. [DOI:10.1016/j.atherosclerosis.2007.03.017]
16. Büttner P, Mosig S, Lechtermann A, Funke H, Mooren FC. Exercise affects the gene expression profiles of human white blood cells. Journal of applied physiology. 2007; 102(1): 26-36. [DOI:10.1152/japplphysiol.00066.2006]
17. Butcher LR, Thomas A, Backx K, Roberts A, Webb R, Morris K. Low-intensity exercise exerts beneficial effects on plasma lipids via PPARgamma. Med Sci Sports Exerc. 2008; 40(7): 1263-70. doi: 10.1249/MSS.0b013e31816c091d. [DOI:10.1249/MSS.0b013e31816c091d]
18. Ghanbari-Niaki A, Khabazian BM, Hossaini-Kakhak SA, Rahbarizadeh F, Hedayati M. Treadmill exercise enhances ABA1 expression in rat liver. Biochemical and biophysical research communications. 2007;361(4):841-6. [DOI:10.1016/j.bbrc.2007.07.100]
19. Bagheri R, Rashidlamir A, Ashtary‐Larky D, Wong A, Alipour M, Motevalli MS, et al. Does Green Tea Extract Enhance the Anti‐inflammatory Effects of Exercise on Fat Loss? Br J Clin Pharmacol. 2019. doi: 10.1111/bcp.14176. [DOI:10.1111/bcp.14176]
20. Matsuura F, Oku H, Koseki M, Sandoval JC, Yuasa-Kawase M, Tsubakio-Yamamoto K, et al. Adiponectin accelerates reverse cholesterol transport by increasing high density lipoprotein assembly in the liver. Biochemical and biophysical research communications. 2007; 358(4): 1091-5. DOI: 10.1016/j.bbrc.2007.05.040. [DOI:10.1016/j.bbrc.2007.05.040]
21. Bagheri R, Moghadam BH, Church DD, Tinsley GM, Eskandari M, Moghadam BH, et al. The effects of concurrent training order on body composition and serum concentrations of follistatin, myostatin and GDF11 in sarcopenic elderly men. Exp Gerontol. 2020; 133: 110869. doi: 10.1016/j.exger.2020.110869. [DOI:10.1016/j.exger.2020.110869]
22. Ghanbari-Niaki A, Saghebjoo M, Rashid-Lamir A, Fathi R, Kraemer RR. Acute circuit-resistance exercise increases expression of lymphocyte agouti-related protein in young women. Experimental Biology and Medicine. 2010; 235(3): 326-34. [DOI:10.1258/ebm.2009.009209]
23. Graf GA, Cohen JC, Hobbs HH. Missense mutations in ABCG5 and ABCG8 disrupt heterodimerization and trafficking. Journal of Biological Chemistry. 2004; 279(23): 24881-8. [DOI:10.1074/jbc.M402634200]
24. Tarr PT, Tarling EJ, Bojanic DD, Edwards PA, Baldán Á. Emerging new paradigms for ABCG transporters. Biochim Biophys Acta. 2009;1791(7):584-93. doi: 10.1016/j.bbalip.2009.01.007. [DOI:10.1016/j.bbalip.2009.01.007]
25. Tofighi A, Rahmani F, Qarakhanlou BJ, Babaei S. The effect of regular aerobic exercise on reverse cholesterol transport A1 and apo lipoprotein aI gene expression in inactive women. Iranian Red Crescent Medical Journal. 2015; 17(4): e26321. doi: 10.5812/ircmj.17(4)2015.26321. [DOI:10.5812/ircmj.17(4)2015.26321]
26. Wang N, Lan D, Chen W, Matsuura F, Tall AR. ATP-binding cassette transporters G1 and G4 mediate cellular cholesterol efflux to high-density lipoproteins. Proceedings of the National academy of Sciences of the United States of America. 2004; 101(26): 9774-9. [DOI:10.1073/pnas.0403506101]
27. Gelissen IC, Harris M, Rye K-A, Quinn C, Brown AJ, Kockx M, et al. ABCA1 and ABCG1 synergize to mediate cholesterol export to apoA-I. Arteriosclerosis, thrombosis, and vascular biology. 2006; 26(3): 534-40. [DOI:10.1161/01.ATV.0000200082.58536.e1]
28. Côté I, Sock ETN, Lévy É, Lavoie J-M. An atherogenic diet decreases liver FXR gene expression and causes severe hepatic steatosis and hepatic cholesterol accumulation: effect of endurance training. European journal of nutrition. 2013; 52(5): 1523-32. [DOI:10.1007/s00394-012-0459-5]
29. Rashidlamir A. Investigation of the Effect of Aerobic and Resistance Exercises on Peripheral Blood Mononuclear Cells ABCG1 Gene Expression in Female Athletes. SSU_Journals. 2012; 20(1): 1-9.
30. Khabazian BM, Ghanbari-Niaki A, Safarzadeh-Golpordesari A, Ebrahimi M, Rahbarizadeh F, Abednazari H. Endurance training enhances ABCA1 expression in rat small intestine. European journal of applied physiology. 2009;107(3):351-8. [DOI:10.1007/s00421-009-1133-3]
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Enamad
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.