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mljgoums 2018, 12(6): 34-39 |
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Mohammadzadeh Rostami F, Shahsafi M. Evaluation and Isolation of Halophilic Bacteria from the Meyghan Lake in Arak, Iran. mljgoums 2018; 12 (6) :34-39
URL: http://mlj.goums.ac.ir/article-1-1136-en.html
URL: http://mlj.goums.ac.ir/article-1-1136-en.html
1- Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2- Department of Microbiology, Science Faculty, Islamic Azad University of Arak, Arak, Iran ,Milad.shahsafiii@gmail.com
2- Department of Microbiology, Science Faculty, Islamic Azad University of Arak, Arak, Iran ,
Abstract: (9009 Views)
ABSTRACT
Background and Objectives: Halophilic bacteria can grow and survive in environments with a wide range a wide range of salinities. In this study, we aimed to isolate halophilic bacteria from the Meyghan Lake in Arak (Iran) and evaluate their enzymatic activity.
Methods: Samples were taken from four different areas of the lake. Halophilic bacteria were isolated by culture in moderate halophilic medium, sea water nutrient agar and nutrient agar containing different salt concentrations. Purification was done via consecutive culture methods, and production of extracellular hydrolytic enzymes including amylase, protease, lecithinase, DNase and lipase was evaluated.
Results: Among 74 bacteria isolated from the lake water samples, 24 produced amylase, 27 produced lipase (Tween 40 and 80), 68 produced protease, three produced DNase and 61 produced lecithinase.
Conclusion: In this study, we isolated halophilic bacteria with enzymatic activity and potential industrial applications.
Keywords: Biological Diversity, isolation and purification, Halobacteriales, Meyghan Lake, Arak.
Background and Objectives: Halophilic bacteria can grow and survive in environments with a wide range a wide range of salinities. In this study, we aimed to isolate halophilic bacteria from the Meyghan Lake in Arak (Iran) and evaluate their enzymatic activity.
Methods: Samples were taken from four different areas of the lake. Halophilic bacteria were isolated by culture in moderate halophilic medium, sea water nutrient agar and nutrient agar containing different salt concentrations. Purification was done via consecutive culture methods, and production of extracellular hydrolytic enzymes including amylase, protease, lecithinase, DNase and lipase was evaluated.
Results: Among 74 bacteria isolated from the lake water samples, 24 produced amylase, 27 produced lipase (Tween 40 and 80), 68 produced protease, three produced DNase and 61 produced lecithinase.
Conclusion: In this study, we isolated halophilic bacteria with enzymatic activity and potential industrial applications.
Keywords: Biological Diversity, isolation and purification, Halobacteriales, Meyghan Lake, Arak.
Research Article: Original Paper |
Subject:
Biochemistry
Received: 2018/11/3 | Accepted: 2018/11/3 | Published: 2018/11/3 | ePublished: 2018/11/3
Received: 2018/11/3 | Accepted: 2018/11/3 | Published: 2018/11/3 | ePublished: 2018/11/3
References
1. Oren A. Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. J Ind Microbiol Biotechnol. 2002; 28(1): 56-63. 2002; 28(1): 56-63.
2. Mellado E, Ventosa A. Biotechnological potential of moderately and extremely halophilic microorganisms. Microorganisms for health care. food and enzyme production. 2003: 233-56.
3. Zhang T, Datta S, Eichler J, Ivanova N, Axen SD, Kerfeld CA, Chen F, et al. Identification of a haloalkaliphilic and thermostable cellulase with improved ionic liquid tolerance. Green Chemistry. 2011; 13(8): 2083-90. [DOI:10.1039/c1gc15193b]
4. de Lourdes Moreno M, Pérez D, García MT, Mellado E. Halophilic bacteria as a source of novel hydrolytic enzymes. Life. 2013 Jan 10;3(1):38-51. [DOI:10.3390/life3010038]
5. microbiology. 2010; 76(21): 6971-81.
6. Oren A. Diversity of halophilic microorganisms: environments, phylogeny, physiology, and applications. Journal of Industrial Microbiology and Biotechnology. 2002; 28(1): 56-63. [DOI:10.1038/sj/jim/7000176]
7. Antón J, Rosselló-Mora R, Rodríguez-Valera F, Amann R. Extremely halophilic bacteria in crystallizer ponds from solar salterns. Applied and Environmental Microbiology. 2000; 66(7): 3052-7. [DOI:10.1128/AEM.66.7.3052-3057.2000]
8. Asad S, Amoozegar MA, Pourbabaee A, Sarbolouki MN, Dastgheib SM. Decolorization of textile azo dyes by newly isolated halophilic and halotolerant bacteria. Bioresource technology. 2007; 98(11): 2082-8. [DOI:10.1016/j.biortech.2006.08.020]
9. Hutcheon GW, Vasisht N, Bolhuis A. Characterisation of a highly stable α-amylase from the halophilic archaeon Haloarcula hispanica. Extremophiles. 2005; 9(6): 487-95. [DOI:10.1007/s00792-005-0471-2]
10. Oie CS, Albaugh CE, Peyton BM. Benzoate and salicylate degradation by Halomonas campisalis, an alkaliphilic and moderately halophilic microorganism. Water research. 2007; 41(6): 1235-42. [DOI:10.1016/j.watres.2006.12.029]
11. Waditee-Sirisattha R, Kageyama H, Takabe T. Halophilic microorganism resources and their applications in industrial and environmental biotechnology. AIMS Microbiol. 2016; 2: 42-54. DOI: 10.3934/microbiol.2016.1.42 [DOI:10.3934/microbiol.2016.1.42]
12. Margesin R, Schinner F. Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles. 2001; 5(2): 73-83. [DOI:10.1007/s007920100184]
13. Yin J, Chen JC, Wu Q, Chen GQ. Halophiles, coming stars for industrial biotechnology. Biotechnol Adv. 2015; 33(7): 1433-42. doi: 10.1016/j.biotechadv.2014.10.008. [DOI:10.1016/j.biotechadv.2014.10.008]
14. Chen GQ, Jiang XR. Next generation industrial biotechnology based on extremophilic bacteria. Current opinion in biotechnology. 2018; 50: 94-100. [DOI:10.1016/j.copbio.2017.11.016]
15. Dalmaso GZ, Ferreira D, Vermelho AB. Marine extremophiles: a source of hydrolases for biotechnological applications. Mar Drugs. 2015; 13(4): 1925-1965. doi: 10.3390/md13041925. [DOI:10.3390/md13041925]
16. Zou H, Chen N, Shi M, Xian M, Song Y, Liu J. The metabolism and biotechnological application of betaine in microorganism. Applied microbiology and biotechnology. 2016; 100(9): 3865-76. doi: 10.1007/s00253-016-7462-3. [DOI:10.1007/s00253-016-7462-3]
17. Kashyap P, Sabu A, Pandey A, Szakacs G, Soccol CR. Extra-cellular L-glutaminase production by Zygosaccharomyces rouxii under solid-state fermentation. Process Biochemistry. 2002; 38(3): 307-12. [DOI:10.1016/S0032-9592(02)00060-2]
18. Karbalaei-Heidari HR, Amoozegar MA, Hajighasemi M, Ziaee AA, Ventosa A. Production, optimization and purification of a novel extracellular protease from the moderately halophilic bacterium Halobacillus karajensis. Journal of industrial microbiology & biotechnology. 2009; 36(1): 21-7. doi: 10.1007/s10295-008-0466-y. [DOI:10.1007/s10295-008-0466-y]
19. Oie CS, Albaugh CE, Peyton BM. Benzoate and salicylate degradation by Halomonas campisalis, an alkaliphilic and moderately halophilic microorganism. Water research. 2007; 41(6): 1235-42. DOI:10.1016/j.watres.2006.12.029. [DOI:10.1016/j.watres.2006.12.029]
20. Calegari-Santos R, Diogo RA, Fontana JD, Bonfim TM. Carotenoid production by halophilic archaea under different culture conditions. Current microbiology. 2016; 72(5): 641-51. doi: 10.1007/s00284-015-0974-8. [DOI:10.1007/s00284-015-0974-8]
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